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Multifunctional bath for in-vitro electrophysiological recording

A multifunctional, bath technology, applied in the field of incubation systems, can solve the problems of low test efficiency and large floor space, and achieve the effects of improving work efficiency, reducing volume, and stable electrophysiological recording.

Pending Publication Date: 2022-01-21
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the present invention provides a small-sized multifunctional bath to solve the problems of large footprint and low test efficiency.

Method used

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  • Multifunctional bath for in-vitro electrophysiological recording
  • Multifunctional bath for in-vitro electrophysiological recording
  • Multifunctional bath for in-vitro electrophysiological recording

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A multifunctional bath for in vitro electrophysiological recording provided according to the present invention includes a box body 100, a water bath 8, a control box 14, an air stone 7 and a bath bath 1. The box body 100 is sequentially connected with a water bath 8 and a control box 9. And the bath 1, the bath 1 realizes internal circulation with the water tank 8 through the water inlet pipe 13 and the water outlet pipe 19, and the control box 14 controls the heating rod 10, the oxygen probe 11, the temperature probe 12 and the pH probe 19 connected to the water tank 8. The air stone 7 is placed in the water tank 8 , and the air stone 7 pumps out 95% oxygen and 5% carbon dioxide to provide oxygen for the aCSF in the water tank 8 .

[0030] The bathtub 1 is provided with a water inlet 16 and a water outlet 17, the water inlet 16 is connected to the water inlet pipe 13 through a three-way water inlet switch 18, the water inlet pipe 13 extends to the water tank 8, the wate...

Embodiment 2

[0036] This Example 2 is completed on the basis of Example 1, and the volume of the incubation system is reduced under the condition of maintaining the integrity of various functions, thereby saving the space of the electrophysiology laboratory, and improving the work efficiency through the use of multiple baths. . specific:

[0037] The box body 100 is made of a transparent white acrylic block with a length of 20cm, a width of 15cm and a height of 15cm. The box body 100 contains 6 bath tubs, and the 6 bath tubs 1 are arranged in an array, and the diameter of each bath tub 1 is 4cm. The water outlet 17 in the bathtub 1 is located 3 cm below the water inlet 16 . The sink 8 is a transparent acrylic sink with a length of 12cm, a width of 9cm and a height of 12cm. The main function is to hold aCSF.

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Abstract

The invention provides a multifunctional bath for in-vitro electrophysiological recording, and relates to the technical field of incubation systems, the multifunctional bath comprises a box body, a water tank, a control box and a bath, the water tank, the control box and the bath are sequentially connected in the box body, and internal circulation of the bath is realized by connecting the water tank with the bath through a water inlet pipe and a water outlet pipe; the control box is in control connection with a heating rod, an oxygen probe, a temperature probe and a PH probe in the water tank. According to the invention, temperature, PH and oxygen of the artificial cerebrospinal fluid in an incubation tank are detected in real time through the temperature sensor, the PH sensor and the oxygen sensor, so that the experimental environment is detected in real time, and adjustment is convenient. Compared with a traditional incubation system, the incubation system provided by the invention can save space, six mouse brain slices can be incubated at the same time, and the experiment efficiency is improved.

Description

technical field [0001] The present invention relates to the technical field of incubation systems, in particular, to a multifunctional bath for in vitro electrophysiological recording. Background technique [0002] The traditional incubation system occupies a large area, and only one bath of an incubation system can incubate mouse brain slices during use, which reduces the experimental efficiency and wastes resources. In the process of use, it is necessary to manually detect the temperature of aCSF, which not only reduces the quality of the incubated brain slices, but also cannot meet the real-time monitoring of the temperature, pH value and dissolved oxygen rate of aCSF during the incubation of mouse brain slices, and then adjust related parameters in real time. . [0003] Through the prior art patent literature search, it is found that the Chinese utility model patent publication number is CN206858581U, which discloses an in vitro brain slice incubation tank, which belong...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/38C12M1/36C12M1/34C12M1/04C12M1/00
CPCC12M29/06C12M41/22C12M41/34C12M41/26
Inventor 李卫东兰兆辉徐文婷杨翔宇曾苏华李雨婷
Owner SHANGHAI JIAO TONG UNIV
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