Polyester carbonic ester anhydride 3D printing bio-ink, and 3D printing method

Abstract

The invention discloses a polyester carbonic ester anhydride 3D printing bio-ink, and a 3D printing method. The polyester carbonic ester anhydride 3D printing bio-ink is composed of a gel ink and a support ink; the gel ink is composed of a hydrogel and biological cells; the hydrogel contains a PLGA-PEG-PLGA triblock copolymer with temperature responsiveness; the support ink is a polyester carbonic ester anhydride copolymer (P(LLA-TMC-SA)copolymer) with surface degradation characteristics. The 3D printing bio-ink possesses thermosensitivity and surface degradable performance, and multicomponent gradient printing and coaxial printing are adopted based on requirements of 3D biologically printed scaffold in vivo degradation structure controllable maintenance so as to obtain models which contain biological cells and are supported by scaffolds with surface controllable degradability, nutrient and metabolism channels with porous structures are obtained, and it is beneficial for obtaining of larger and thicker tissue structures via 3D printing.

Description

technical field [0001] The present invention relates to the field of biotechnology, especially the field of 3D bioprinting technology, in particular to a surface-degradable polyester carbonate anhydride 3D printing bioink and a 3D printing method. Background technique [0002] 3D printing can quickly and efficiently manufacture personalized products, so it is gradually being introduced into the biomedical industry, with a view to using 3D printing technology for tissue and organ transplantation. "The Latest Research and Application of Bio-3D Printing" (Powder Metallurgy Industry, Volume 25, No. 4, August 2015, Zhang Hongbao, etc.) introduced the application of 3D bio-printing technology into different levels, which is quoted here as refer to. The basic method of higher-level 3D bioprinting is to inoculate living cells into a biocompatible and finally biodegradable scaffold, and then culture them in a bioreactor, so that the cells rely on the space formed by the scaffold to ...

AI Technical Summary

Problems solved by technology

[0005] In fact, it is precisely because of the above reasons that the existing 3D bioprinting technology can only print a very thin layer of tissue, because there are no nutrient channels such as blood vessels in the thicker tissue, it is difficult for the internal cells to obtain nutrients, and the metabolites cannot be discharged. Thicker 3D printed tissue struggles to sustain growth