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Production method of biodegradable nanometer fiber diaphragm applied to neurosurgery indirect vascular bypass

A nanofiber membrane and neurosurgery technology, applied in the field of nanobiomedicine, can solve the problems of lack of angiogenesis, stimulation, and inability to act directly, and achieve good biocompatibility

Active Publication Date: 2017-02-15
SHANGHAI TONGJI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the local release of VEGF is helpful to improve angiogenesis after indirect extracranial vascular bypass, but how to effectively control the release of VEGF in real time to promote the "on-demand growth" of blood vessels in the ischemic brain tissue is still lacking an effective method.
Moreover, the local injection cannot directly act on the tissue interface of the indirect vascular bypass, and lacks a continuous stimulating effect on the angiogenesis of the cerebral cortex.
[0006] In summary, in the reports of the existing literature, there is no biodegradable nanofibrous membrane described in the present invention, and it promotes blood vessels and (or) nerves in ischemic brain tissue in a manner of controlled release of growth factors. Relevant research reports on regeneration

Method used

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  • Production method of biodegradable nanometer fiber diaphragm applied to neurosurgery indirect vascular bypass
  • Production method of biodegradable nanometer fiber diaphragm applied to neurosurgery indirect vascular bypass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Using PCL as a raw material, it was dissolved in a mixed solution of chloroform / N,N-dimethylformamide (volume ratio 9:1) to prepare a solution with a concentration of 6% (wt). Add this mixture solution to the electrospinning syringe controlled by the syringe pump, set the voltage of the high-voltage generator to 15KV, and the collection distance to 15cm, and a membrane composed of ultrafine fibers with a diameter of nanometer scale can be obtained, and then the The membrane was washed several times with deionized water and dried in vacuum before use. The dried membrane is immersed in polyallylamine (PAH, 0.1-1 mg / ml) solution for 30-60 minutes, and then soaked in deionized water for 10 minutes. Then VEGF and NGF are made into a mixed growth factor solution with a concentration of 0.1-10 μg / ml, the membrane is soaked in the growth factor solution for 10-20 minutes for adsorption, and then washed with deionized water. Then immerse in polystyrene sodium sulfonate (PSS, 0....

Embodiment 2

[0032] PLGA is used as a raw material and dissolved in hexafluoroisopropanol to prepare a solution with a concentration of 7% (wt). Add this mixture solution to the electrospinning syringe controlled by the syringe pump, set the voltage of the high-voltage generator to 14KV, and the collection distance to 15cm, a membrane composed of ultrafine fibers with a diameter of nanoscale can be obtained, and then the The membrane was washed several times with deionized water and dried in vacuum before use. The dried membrane is immersed in polyallylamine (PAH, 0.1-1 mg / ml) solution for 30-60 minutes, and then soaked in deionized water for 10 minutes. Then VEGF and NGF are made into a mixed growth factor solution with a concentration of 0.1-10 μg / ml, the membrane is soaked in the growth factor solution for 10-20 minutes for adsorption, and then washed with deionized water. Then immerse in polystyrene sulfonate sodium (PSS, 0.1-1mg / ml) solution, growth factor solution and chitosan (Chit...

Embodiment 3

[0034] PCL and collagen (collagen) were used as raw materials (ratio: 2:1), dissolved in hexafluoroisopropanol to prepare a solution with a concentration of 7% (wt). Add this mixture solution to the electrospinning syringe controlled by the syringe pump, set the voltage of the high-voltage generator to 15KV, and the collection distance to 15cm, and a membrane composed of ultrafine fibers with a diameter of nanometer scale can be obtained, and then the The membrane was washed several times with deionized water and dried in vacuum before use. The dried membrane is immersed in polyallylamine (PAH, 0.1-1 mg / ml) solution for 30-60 minutes, and then soaked in deionized water for 10 minutes. Then VEGF and NGF are made into a mixed growth factor solution with a concentration of 0.1-10 μg / ml, the membrane is soaked in the growth factor solution for 10-20 minutes for adsorption, and then washed with deionized water. Then immerse in sodium alginate / PSS mixed solution (sodium alginate, 0...

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Abstract

The invention relates to a biodegradable nanometer fiber diaphragm applied to neurosurgery indirect vascular bypass. The biodegradable nanometer fiber diaphragm can be produced through the following steps: dissolving a biodegradable polymer material in a polar and volatile organic solvent, carrying out electrostatic spinning to produce a diaphragm composed of ultrafine fibers with the nano-scale diameters; and loading growth factors for promoting brain tissue blood vessel regeneration and / or promoting nerve regeneration to the diaphragm through a layer and layer self-assembling technology, and drying the factor growth supported diaphragm. The biodegradable nanometer fiber diaphragm has the advantages of no toxicity, no generation of harmful substances after in vivo degradation, and good biocompatibility. The biodegradable nanometer fiber diaphragm can avoid growth factor inactivation caused by organic solvents, strong acids, strong alkalis and high temperature, and an assembled polyelectrode can guarantee the good activity of the growth factor supported on the diaphragm. The biodegradable nanometer fiber diaphragm can realize long-term controlled release of VEGF, NGF and other growth factors in the brain blood vessel obstruction position in order to induce blood vessel regeneration and nerve regeneration of the brain tissue blood vessel obstruction position.

Description

technical field [0001] The invention relates to the technical field of nanometer biomedicine, in particular to a biodegradable nanofiber membrane applied to indirect vascular bypass in neurosurgery and a preparation method thereof. Background technique [0002] Ischemic cerebrovascular disease is a major disease that directly threatens human health. It is common in intracranial and extracranial vascular stenosis or occlusion, cerebral arteriosclerotic small vessel disease, and moyamoya disease [Qiu L, Ng G, Tan EK, et al. Chronic cerebral hypoperfusion enhances Tau hyperphosphorylation and reduces autophagy in Alzheimer's disease mice.Sci Rep,2016;6:23964.Araki Y,Takagi Y,Ueda K,etal.Cognitive function of patients with adult moyamoya disease.J Stroke Cerebrovasc Dis,2014;9-23:17 .Su SH,Hai J,Zhang L,et al.Assessment of cognitive function in adult patients with hemorrhagic moyamoya disease who received no surgical revascularization.Eur J Neurol,2013;20:1081-1087.], long-term ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/24A61L27/50A61L27/58A61L27/54D06M15/356D06M15/15D06M15/03D06M101/12D06M101/32
CPCA61L27/18A61L27/24A61L27/50A61L27/507A61L27/54A61L27/58A61L2300/414A61L2430/32C08L89/00D06M15/03D06M15/15D06M15/3562D06M2101/12D06M2101/32C08L67/04
Inventor 海舰赵鹏韩斐吴一芳王大鹏苏少华
Owner SHANGHAI TONGJI HOSPITAL
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