Method for confirming tumour differentiation grade

A grade, tumor technology, applied in the grade field, can solve problems such as unclear

Inactive Publication Date: 2006-04-26
F HOFFMANN LA ROCHE & CO AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the genes and / or proteins that characterize and / or regulate the various grades of HCC differentiation during the tumorigenesis and progression of HCV-associated HCC are unclear.

Method used

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  • Method for confirming tumour differentiation grade
  • Method for confirming tumour differentiation grade
  • Method for confirming tumour differentiation grade

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Embodiment Construction

[0011] In the present invention, human hepatocellular carcinoma (HCC) tissue and non-tumor (non-cancerous or precancerous) liver tissue are used. Analyze HCCs using HCCs infected with HCV. The presence of HCV and / or HBV infection can be detected by immunoreactivity against anti-HCV antibodies and anti-HBV antibodies or by amplifying the HCV and / or HBV genome by PCR. The differentiation grade of HCC can be detected by histopathological examination. HCCs are divided into well differentiated HCC (G1), moderately differentiated HCC (G2) and poorly differentiated HCC (G3). Non-tumor liver samples can be obtained from patients who have undergone liver resection due to benign or metastatic liver tumors. Liver samples without HCV infection are classified as non-cancerous liver (L0), and liver samples with HCV infection are classified as precancerous liver (L1). After the liver tissue is removed in a surgical operation, it is preferable to immediately freeze the tissue in liquid nitrogen o...

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Abstract

The present invention relates to a method of defining the differentiation grade of tumor by selecting genes and / or proteins whose expression level correlates with each differentiation grade of tumor, measuring the expression of the genes and / or proteins of human tumor tissues in each differentiation grade. The present invention also relates to the use of these genes and / or proteins for diagnosing the differentiation grade of tumor and for screening anti-cancer agents for tumor treatment.

Description

Technical field [0001] The present invention relates to a method for determining the grade of tumor differentiation. More particularly, the present invention relates to the determination of tumors by screening the genes and / or proteins whose expression levels are associated with each differentiation level of hepatocellular carcinoma (HCC), and determining the expression of genes and / or proteins in human tumor tissues in each differentiation level. The method of differentiation hierarchy. The present invention also relates to the use of these genes and / or proteins in diagnosing the differentiation level of HCC and screening anticancer agents for HCC treatment. [0002] The present invention also relates to a kit for implementing the method of the present invention, which includes DNA microarray, oligonucleotide microarray, protein array, northern blotting, in situ hybridization, RNase protection test, western blotting ( Western blotting), ELISA test, reverse transcription polymeras...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/574
CPCC12Q1/6886C12Q2600/112C12Q2600/136G01N33/57484A61P43/00
Inventor 冈正朗浜本义彦饭塚德男冈部尚文浜田健嗣
Owner F HOFFMANN LA ROCHE & CO AG
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