Method for confirming tumour differentiation grade

一种试剂盒、等级的技术，应用在级领域，能够解决不清楚等问题

Inactive Publication Date: 2012-08-29
F HOFFMANN LA ROCHE & CO AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the genes and / or proteins that characterize and / or regulate the various grades of HCC differentiation during the tumorigenesis and progression of HCV-associated HCC are unclear.

Method used

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  • Method for confirming tumour differentiation grade
  • Method for confirming tumour differentiation grade
  • Method for confirming tumour differentiation grade

Examples

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Embodiment Construction

[0011] In the present invention, human hepatocellular carcinoma (HCC) tissue and non-neoplastic (non-cancerous or precancerous) liver tissue were utilized. HCCs were analyzed using HCCs infected with HCV. The presence of HCV and / or HBV infection can be detected by immunoreactivity against anti-HCV antibodies and anti-HBV antibodies or by amplification of the HCV and / or HBV genome using PCR. The differentiation grade of HCC can be detected by histopathological examination, and HCCs are divided into well-differentiated HCC (G1), moderately differentiated HCC (G2) and poorly differentiated HCC (G3). Non-neoplastic liver samples can be obtained from patients undergoing liver resection for benign or metastatic liver tumors. Liver samples without HCV infection were classified as noncancerous liver (L0), and liver samples with HCV infection were classified as precancerous liver (L1). Immediately after surgical resection of liver tissue, the tissue is preferably frozen in liquid nit...

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Abstract

The present invention relates to a method of defining the differentiation grade of tumor by selecting genes and / or proteins whose expression level correlates with each differentiation grade of tumor, measuring the expression of the genes and / or proteins of human tumor tissues in each differentiation grade. The present invention also relates to the use of these genes and / or proteins for diagnosingthe differentiation grade of tumor and for screening anti-cancer agents for tumor treatment.

Description

technical field [0001] The present invention relates to methods for determining the grade of tumor differentiation. More particularly, the present invention relates to determining the expression of genes and / or proteins in human tumor tissues in various differentiation levels by screening genes and / or proteins whose expression levels are related to each differentiation level of hepatocellular carcinoma (HCC) A method of classifying grades. The present invention also relates to the use of these genes and / or proteins in diagnosing the differentiation grade of HCC and screening anticancer agents for HCC treatment. [0002] The present invention also relates to kits for implementing the methods of the present invention, which include DNA microarrays, oligonucleotide microarrays, protein arrays, Northern blotting (northern blotting), in situ hybridization, RNase protection assay, Western blotting ( DNA chips, oligonucleotide chips, protein chips, peptides, antibodies, probes and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N33/574
CPCC12Q2600/112C12Q1/6886G01N33/57484C12Q2600/136A61P43/00
Inventor 冈正朗浜本义彦饭塚德男冈部尚文浜田健嗣
Owner F HOFFMANN LA ROCHE & CO AG
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