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Method for determining low-concentration protein in secondary output water of sewage plant

A technology for secondary effluents and sewage plants, applied in color/spectral characteristic measurement, special data processing applications, instruments, etc., can solve the problems of low precision and low accuracy of protein determination, and achieve strong applicability, wide application range, easy-to-handle effects

Active Publication Date: 2015-01-21
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the problems of low sensitivity, low measurement accuracy and precision, inconvenient operation and small scope of application in the determination of protein content in the prior art, the present invention provides a method for the determination of low-concentration protein in the secondary effluent of a sewage plant. The surface method is used to analyze the protein content, which overcomes the problems of low accuracy and precision in the determination of protein in real sewage. This detection method is simple and easy to operate, and is suitable for the determination of low-concentration protein in the secondary effluent of urban domestic sewage plants

Method used

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  • Method for determining low-concentration protein in secondary output water of sewage plant
  • Method for determining low-concentration protein in secondary output water of sewage plant
  • Method for determining low-concentration protein in secondary output water of sewage plant

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Embodiment 1

[0037] A method for measuring low-concentration protein in secondary effluent water of a sewage plant, the steps of which are:

[0038] (a) Preparation of reagents: 10g Na 2 CO 3 and 2g NaOH were dissolved in 500mL distilled water to obtain reagent A, 0.781gCuSO 4 ·5H 2 O and 1g of antimony potassium tartrate were dissolved in 100mL of distilled water to obtain reagent B, and reagent C was obtained by mixing 49mL of reagent A and 2mL of reagent B. It is now ready for use. The volume ratio of folin phenol and distilled water is 3:1 to obtain folin Phenol use liquid;

[0039] (b) Establish a mathematical model: use the Minitab response surface method to establish a mathematical model between the concentration of bovine serum albumin BSA, humic acid HS and the corresponding absorbance value, the specific steps are:

[0040] (1) BSA standard solution and HS standard solution with concentration of 62.5mg / L were prepared respectively with distilled water, for subsequent use;

...

Embodiment 2

[0054] With embodiment 1, difference is that the sample in the step (c) is a certain sewage treatment plant in Wuxi (A 2 / O-MBR process) secondary effluent, the dialysis time is 2.5h; Utilize the mathematical model formula C established in the step (b) according to the absorbance value measured BSA =158.92*A 2 -149.61*A 1 -0.087 calculates that the protein concentration of this water sample is 1.71mg / L.

[0055] In order to improve the accuracy and reliability of determination, the present embodiment repeatedly measured three times to get the average value, and the results are as attached Figure 4 WX(A 2 / O-MBR), the measurement results are accurate.

Embodiment 3

[0057] With embodiment 1, difference is that the sample in the step (c) is a sewage treatment plant in Nanjing (A 2 / O process) secondary effluent, the dialysis time is 3h; according to the measured absorbance value, the mathematical model established in step (b) is used to calculate the protein concentration of the water sample to be 2.09mg / L.

[0058] In order to improve the accuracy and reliability of determination, the present embodiment repeatedly measured three times to get the average value, and the results are as attached Figure 4 NJ in (A 2 / O), the measurement results are accurate.

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Abstract

The invention discloses a method for determining low-concentration protein in secondary output water of a sewage plant, belonging to the field of municipal sewage treatment. The method comprises the following steps: preparing a reagent, establishing a mathematical model, pretreating a sample, determining the concentration of the protein and the like. Based on a Lowry method and an improved Lowery method, a Minitab response surface method is used for analyzing the content of the protein so that the problems of low accuracy and low precision of the determination of the protein in real sewage are overcome; and the interferences on humic acid, carbohydrates, and ions including Ca<2+>, Mg<2+> and the like in the secondary output water are reduced. The method for determining the low-concentration protein in the secondary output water of the sewage plant has the advantages of high accuracy, simplicity, easiness for operation and the like, is suitable for determining the low-concentration protein in the secondary output water of the sewage plant, and can be used for synchronously determining the concentration of the humic acid in the secondary output water, so that the applicable range is wide.

Description

technical field [0001] The invention belongs to the field of urban domestic sewage treatment, and in particular relates to a method for measuring low-concentration protein in secondary effluent water of a sewage plant. Background technique [0002] Dissolved organic nitrogen (DON) generally exists in the secondary effluent of biochemical treatment and is part of dissolved organic matter. DON is an important component of effluent total nitrogen, if not controlled, it will aggravate the eutrophication of rivers. DON can also cause membrane fouling, and will produce highly carcinogenic nitrosamine compounds during the chlorination process, posing a major threat to water quality safety. Sewage treatment plants are important sites for DON degradation and discharge. Therefore, the monitoring and control of DON in the secondary effluent has become one of the concerns in the fields of sewage recycling and drinking water. Studies have shown that effluent protein is the main compone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G06F19/00
Inventor 任洪强胡海冬丁丽丽耿金菊许柯张宴
Owner NANJING UNIV
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