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C-reactive protein determination reagent and preparation method thereof

A technology for reacting proteins and detection reagents, applied in the field of biological reagents, can solve problems such as application limitations, increase the cost of diagnosis and analysis, and achieve the effects of high accuracy, remarkable effect and high measurement accuracy

Inactive Publication Date: 2019-07-19
贵州盛世康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, none of the reagents in the prior art can cover the above reference ranges at the same time, resulting in application limitations and increased diagnostic analysis costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] This embodiment relates to a C-reactive protein assay reagent, including C-reactive protein detection reagent R1, C-reactive protein detection reagent R2 and C-reactive protein calibrator;

[0041] The components and content of the C-reactive protein detection reagent R1 are: 0.02% stabilizer, 10mM buffer, 0.02% preservative;

[0042] The components and content of the C-reactive protein detection reagent R2 are: 0.01% stabilizer, 1mg / ml C-reactive protein immune latex microspheres, 10mM buffer, 0.02% preservative;

[0043] C-reactive protein calibrator, its components and contents are: 0.01% calibrator stabilizer, 20mg / L C-reactive protein, 10mM buffer, 0.02% calibrator preservative;

[0044] Wherein, the stabilizer is 0.6% sodium chloride;

[0045] The buffer is 4-hydroxyethylpiperazineethanesulfonic acid-sodium hydroxide buffer, trishydroxymethylaminomethane-hydrochloric acid buffer, 3-(N-morpholino)-2-hydroxypropanesulfonic acid- Sodium hydroxide buffer and phospha...

Embodiment 2

[0059] This embodiment relates to a C-reactive protein assay reagent, including C-reactive protein detection reagent R1, C-reactive protein detection reagent R2 and C-reactive protein calibrator;

[0060] The components and content of the C-reactive protein detection reagent R1 are: 10% stabilizer, 100mM buffer, 0.05% preservative;

[0061] The components and content of the C-reactive protein detection reagent R2 are: 15% stabilizer, 3mg / ml C-reactive protein immune latex microspheres, 100mM buffer, 0.05% preservative;

[0062] C-reactive protein calibrator, its components and content are: 13% calibrator stabilizer, 20mg / L C-reactive protein, 200mM buffer, 0.1% calibrator preservative;

[0063] Wherein, the stabilizer is 1% BSA;

[0064] The buffer is 4-hydroxyethylpiperazineethanesulfonic acid-sodium hydroxide buffer, trishydroxymethylaminomethane-hydrochloric acid buffer, 3-(N-morpholino)-2-hydroxypropanesulfonic acid- Sodium hydroxide buffer and phosphate buffer are prepa...

Embodiment 3

[0079] This embodiment relates to a C-reactive protein assay reagent, including C-reactive protein detection reagent R1, C-reactive protein detection reagent R2 and C-reactive protein calibrator;

[0080] The components and content of the C-reactive protein detection reagent R1 are: 8% stabilizer, 50mM buffer, and 0.03% preservative.

[0081] The components and contents of the C-reactive protein detection reagent R2 are: 10% stabilizer, 2mg / ml C-reactive protein immune latex microspheres, 85mM buffer, and 0.04% preservative.

[0082] C-reactive protein calibrator, its components and contents are: 5% calibrator stabilizer, 20mg / L C-reactive protein, 20mM buffer, 0.02-0.1% calibrator preservative;

[0083] Wherein, the stabilizer is one of 0.6-1.5% sodium chloride and 0.1-1% BSA;

[0084] The buffer is 4-hydroxyethylpiperazineethanesulfonic acid-sodium hydroxide buffer, trishydroxymethylaminomethane-hydrochloric acid buffer, 3-(N-morpholino)-2-hydroxypropanesulfonic acid- Sodi...

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Abstract

The invention provides a C-reactive protein determination reagent. The C-reactive protein determination reagent comprises a C-reactive protein detection reagent R1, a C-reactive protein detection reagent R2 and a C-reactive protein calibrator; buffer solution is prepared from 4- hydroxyethylpiperazine ethane sulfonic acid-sodium hydroxide buffer solution, trihydroxymethyl aminomethane-hydrochloricacid buffer solution, 3-(N-morpholinyl)-2-hydroxyl propanesulfonic acid-sodium hydroxide buffer solution and phosphate buffer solution according to mass ratio of 1:1:1:1; the C-reactive protein immune gel emulsion microspheres are polystyrene microspheres, preservative is one or two of proclin 300, gentamicin and chloramphenicol. The buffer solution used for the reagent provided by the inventionis prepared from the 4- hydroxyethylpiperazine ethane sulfonic acid-sodium hydroxide buffer solution, the trihydroxymethyl aminomethane-hydrochloric acid buffer solution, the 3-(N-morpholinyl)-2-hydroxyl propanesulfonic acid-sodium hydroxide buffer solution and the phosphate buffer solution according to mass ratio of 1:1:1:1, and the reagent is remarkable effects; and the reagent has the advantages of high accuracy, high measurement precision, high analysis sensitivity, and so on.

Description

technical field [0001] The invention relates to the field of biological reagents; in particular, it relates to a C-reactive protein assay reagent and a preparation method thereof. Background technique [0002] Immunoanalysis technology is now widely used in the field of biomedicine, and has become an essential conventional technical means in many fields such as clinical diagnosis, environmental and health testing, forensic identification, food safety, etc., playing an irreplaceable role and has huge market. The signal system used by immunoassay reagents plays a decisive role in the performance of immunoassay reagents, from radioimmunoassay (RIA) to enzyme-linked immunosorbent assay (ELISA) to chemiluminescent immunoassay (CLIA), immunoassay technology The development and progress are mainly the progress and revolution of the signaling system. The basis of the immune response using antigens and antibodies and their various conjugates in immunoassay technology has not changed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/54313G01N33/6893G01N2333/4737
Inventor 赵海峰蒙凯李俊英李家维
Owner 贵州盛世康生物科技有限公司
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