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Milk product true protein determinator and determination method thereof

A method for measuring true protein, which is applied in the field of true protein measuring instruments for dairy products, can solve the problems of complex detection process, high requirements for instruments, and high cost of instruments, and achieve the effect of reducing errors and ensuring safety

Inactive Publication Date: 2015-11-18
SICHUAN KAFU DETECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, flow spectrophotometry can automatically quantify samples and reagents at the same time. This method has the advantages of fast analysis speed, low reagent consumption, low detection limit, and good reproducibility. This method has been successfully used for protein content in dairy products. The accurate determination of the protein content in dairy products meets the needs of the market for simple, fast and accurate determination of protein content in dairy products, but the detection process is complicated, the requirements for instruments are high, and the cost of purchasing instruments is high, so it is difficult to realize the detection of dairy products in the market. On-site rapid analysis
In summary, the above methods have limitations

Method used

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Experimental program
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Embodiment 1

[0020] Embodiment 1: preparation instrument: JPRS-1 dissolved oxygen instrument; Magnetic stirrer; Kjeldahl nitrogen device (laboratory configuration); Analytical balance (1 / 10,000 balance); Pure water machine; Glycine standard substance (analytical pure, 100g); Glutaraldehyde (analytical pure, 25%); Dipotassium hydrogen phosphate (analytical pure); Potassium dihydrogen phosphate (analytical pure); Potassium chloride saturated solution (analytical pure potassium chloride crystals are dissolved in water for preparation); Concentrated sulfuric acid (provided by the laboratory); copper sulfate (analytical pure); potassium sulfate (analytical pure). The reagents were of analytical grade unless otherwise specified, and the water used in this experiment was pure water.

[0021] Zero oxygen calibration: Weigh 5.26g of sodium sulfite and dissolve it in 100mL of pure water to prepare a 5% sodium sulfite solution for later use. Press the "Zero Oxygen" key of the dissolved oxygen meter,...

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Abstract

The invention discloses a milk product true protein determinator and a determination method thereof, and belongs to the technical field of milk product true protein detection. The milk product true protein determinator comprises a potentiometer, a dissolved oxygen electrode, a test cup body, a magnetic stirrer and a current detection device; the bottom of the test cup body is communicated with and fixed on the magnetic stirrer, a stirring rod located on the magnetic stirrer is arranged in an inner cavity of the test cup body, the dissolved oxygen electrode is connected with the potentiometer through a wire, the bottom of the dissolved oxygen electrode stretches into the inner cavity of the test cup body, the current detection device is used for detecting currents of a solution in the inner cavity of the test cup body, and the potentiometer, the dissolved oxygen electrode, magnetic stirrer and the current detection device are controlled by a control system to work. According to the milk product true protein determinator and the determination method thereof, the content of protein in milk products is quickly determined by means of a current method, the content of the protein in the sample is analyzed by determining the current decrease value through a dissolved oxygen meter, and therefore the detection errors caused by existence of non-protein nitrogen are reduced.

Description

technical field [0001] The invention belongs to the technical field of dairy product true protein detection, and in particular relates to a dairy product true protein measuring instrument and a measuring method thereof. Background technique [0002] Dairy products refer to products that use cow's milk or goat's milk and their processed products as the main raw materials, with or without adding appropriate amounts of vitamins, minerals and other auxiliary materials, and use the conditions required by laws, regulations and standards to process products. Protein is an indispensable and important nutrient for the human body, and dairy products provide us with most of the protein sources. Therefore, the determination of protein in dairy products is an important index for evaluating the quality of dairy products. At the same time, protein is also an important nutritional index in many other foods. Determination of protein content in food plays an extremely important role in evalu...

Claims

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Application Information

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IPC IPC(8): G01N27/00G01N1/38
Inventor 陈浩郑波
Owner SICHUAN KAFU DETECTION TECH CO LTD
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