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CRP (C-Reactive Protein) assay kit, and preparation method and application thereof

A reactive protein and reagent kit technology, applied in biological testing, analysis through chemical reactions of materials, measuring devices, etc., can solve problems affecting measurement results, reagent freezing and thawing, reagent inactivation, etc., to achieve high detection sensitivity, Low cost and good precision

Active Publication Date: 2020-01-03
广州市伊川生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the refrigerated (insulated) box, the reagent boxes and ice packs are placed irregularly. If the reagents that need to be refrigerated are mixed with ice packs, there may be a risk of inactivation of some factors in the reagents due to freezing and thawing of the reagents, thereby affecting Therefore, solving the problem of freezing and thawing of reagents during transportation has become a big problem

Method used

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  • CRP (C-Reactive Protein) assay kit, and preparation method and application thereof
  • CRP (C-Reactive Protein) assay kit, and preparation method and application thereof
  • CRP (C-Reactive Protein) assay kit, and preparation method and application thereof

Examples

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Embodiment 1

[0042] This embodiment is a C-reactive protein (CRP) assay kit of the present invention

[0043] A C-reactive protein (CRP) assay kit, comprising reagent R1 and reagent R2;

[0044] Components included in reagent R1:

[0045] TRIS / HCl buffer 100mmoL / L NaCl 25g / L Tween-20 (Tween-20) 10g / L sucrose 10g / L Sodium azide 0.5g / L

[0046] The components included in reagent R2 are as follows:

[0047]

[0048]

[0049] The preparation method of the above-mentioned C-reactive protein (CRP) assay kit: each component in the above-mentioned reagent R1 is prepared according to a conventional method, and the TRIS / HCL buffer solution, Tween-20 (Tween-20), calf Serum albumin (BSA), erythritol and sodium azide are prepared according to conventional methods; the preparation method of the anti-human CRP antibody latex conjugate in the above-mentioned R2 reagent comprises the steps:

[0050] The preparation method of the latex conjugate of the ant...

Embodiment 2

[0057] This embodiment is a C-reactive protein (CRP) assay kit of the present invention

[0058] A C-reactive protein (CRP) assay kit, comprising reagent R1 and reagent R2;

[0059] Components included in reagent R1:

[0060] TRIS / HCl buffer 100mmoL / L NaCl 25g / L Tween-20 (Tween-20) 10g / L sucrose 10g / L Sodium azide 0.5g / L

[0061] Components included in reagent R2:

[0062] TRIS / HCL buffer 150mmoL / L Tween-20 (Tween-20) 10g / L Bovine Serum Albumin (BSA) 25g / L Anti-human CRP antibody latex conjugate 10g / L Sodium azide 0.5g / L

[0063] The preparation method of the above-mentioned C-reactive protein (CRP) assay kit: each component in the above-mentioned reagent R1 is prepared according to a conventional method, and each component in the above-mentioned reagent R2 is prepared according to a conventional method except for the latex conjugate of anti-human CRP antibody The preparation method of th...

Embodiment 3

[0070] This embodiment is a C-reactive protein (CRP) assay kit of the present invention

[0071] A C-reactive protein (CRP) assay kit, comprising reagent R1 and reagent R2;

[0072] Components included in reagent R1:

[0073]

[0074]

[0075] The components included in reagent R2 are as follows:

[0076] Glycine buffer 150mmoL / L Phosphate buffer 50mmoL / L Tween 80 25g / L casein 25g / L Anti-human CRP antibody latex conjugate 7g / L Erythritol 10g / L Thimerosal 2g / L

[0077] The preparation method of the above-mentioned C-reactive protein (CRP) assay kit: each component in the above-mentioned reagent R1 is prepared according to a conventional method, and each component in the above-mentioned reagent R2 is prepared according to a conventional method except for the latex conjugate of anti-human CRP antibody The preparation method of the anti-human CRP antibody latex conjugate in the above-mentioned reagent R2, comprising...

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Abstract

The invention relates to a CRP (C-Reactive Protein) assay kit, and a preparation method and application thereof, and belongs to the technical field of IVD reagents (In Vitro Diagnostic Reagents). TheCRP assay kit provided by the invention comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises buffer solutions, inorganic salts, surfactants, stabilizing agents and preservatives;and the reagent R2 comprises buffer solutions, surfactants, sealing agents, anti-human CRP antibody latex conjugates and preservatives. The CRP assay kit provided by the invention has the advantages that on the basis of the existing latex immunoturbidimetry, a reaction system is optimized by a specific preparation method through an anti-freezing agent; the detection accuracy, the analysis sensitivity and precision of tested reagents are further improved; the research and development cost of the reagents are not improved; and the CRP assay kit can easily adapt to the development of the modern clinical laboratory medicine.

Description

technical field [0001] The invention relates to a C-reactive protein (CRP) assay kit and a preparation method and application thereof, belonging to the technical field of in vitro diagnostic reagents. Background technique [0002] C-reactive protein (CRP) is a protein (acute protein) that rises sharply in plasma when the body is infected or tissue damaged. It is a protein substance discovered by Tillet and Francis in 1930. At first they observed that some acute patient's serum could react with the capsular C-polysaccharide of Streptococcus pneumoniae, and then confirmed that the substance that could react with C-polysaccharide was a protein, so they named it C-reactive protein (C-reactive protein). reactive protein, CRP). C-reactive protein (CRP) is non-covalently linked by 5 identical non-glycosylated subunits, each subunit has a relative molecular mass of 23017 and consists of 206 amino acid residues. The body characteristic structure makes it belong to the pentraxin (Pe...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543G01N33/536G01N21/82G01N33/531
CPCG01N33/6854G01N33/54313G01N33/536G01N33/531G01N21/82G01N2800/7095
Inventor 刘光华杨玉军
Owner 广州市伊川生物科技有限公司
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