Method for evaluating influences of biological protein on apolygus lucorum

Abstract

The invention provides a method for evaluating influences of biological protein on apolygus lucorum. The method includes the steps of firstly, diluting biological protein to be measured to obtain biological protein with different concentrations, mixing the biological protein with apolygus lucorum artificial fodders to obtain biological protein measurement fodders, and taking fodders where biological protein is not added as a contrast; secondly, wrapping the measurement fodders and the contrast fodders with extended sealing films respectively to obtain capsules; thirdly, adhering the capsules to the side wall of a raising box without cover; fourthly, inoculating nymphs of apolygus lucorum into the raising box, sealing the opening of the raising box through gauze, and conducting cultivation; fifthly, recording the death condition of apolygus lucorum or the weight changes of apolygus lucorum from the day 0, and thereby evaluating the influences of biological protein on apolygus lucorum. The method is wide in application range, agents with stomach toxicity and systemic action and chemical substances dissolved in the artificial fodders can be effectively measured, the problem that piercing-sucking mouthpart insects including apolygus lucorum can hardly make direct contact with biological protein with different concentrations is solved, the influences of biological protein on apolygus lucorum can be accurately measured, and the method has high practicability.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a method for evaluating the influence of biological protein on Lygus aeruginosa. Background technique [0002] Mirid bugs are an important class of agricultural pests. In my country, there are mainly Apolygus lucorum Meyer-Dür, Adelphocoris suturalis Jakovlev, Adelphocoris lineolatus Goeze, and Three-spotted Lygus (Adelphocoris suturalis Jakovlev). Adelphocoris fasciaticollis Reuter) and several others. In the latter half of the 20th century, the occurrence of Lygus miridae in cotton fields in my country was very low, and it has been controlled as a concurrent chemical control object of cotton bollworm (Helicoverpa armigera Hübner). At the end of the 1990s, my country began to commercially plant transgenic Bt cotton, and the cotton bollworm was effectively controlled. However, the occurrence of mirid bugs, which are secondary pests in cotton fields, has incre...

AI Technical Summary

Problems solved by technology

[0004] The green Lygus is a piercing-sucking pest, and its feeding characteristics are quite different from those of the chewing pests. Moreover, the nymphs and adults of the green Lygus are relatively active, so it is difficult to conduct bioassays; the subculture of the green Lygus is usually It uses fresh green beans or corn to raise indoors, and the measured protein can only exist on the surface of green beans or corn through soaking, smearing, etc., and it is difficult to be sucked by the green ligus.

If transgenic cotton and other plants are used to evaluate the impact of biological proteins on Lygus spp., it can only limit the concentration of proteins expressed in plants within a certain range, and it is difficult to accurately evaluate the impact of different protein concentrations, especially high-concentration proteins on Lygus spp.

Images