The invention provides a cannabidiol liquid preparation with the anti-anxiety effect and a preparation method of the cannabidiol liquid preparation. The cannabidiol liquid preparation is prepared from the following raw materials in parts by weight: 5-10 parts of cannabidiol, 3-5 parts of rhizoma ligustici wallichii, 3-5 parts of semen ziziphi spinosae, 3-5 parts of cortedx moutan, 1-2 parts of corydalis tuber, 1-2 parts of radix rumicis nepalensis, 1-2 parts of herba hyperici perforati cum radice, 1-2 parts of rhizoma gastrodiae, 1-2 parts of stigma croci sativi, 1-2 parts of herba schizonepetae, 1-2 parts of herba dianthi, 1-2 parts of herba thlaspis arvensis, 1-2 parts of ramulus euonymi alati, 1-2 parts of flos jasmini grandiflori immaturus, 1-2 parts of os sepiae, 1-3 parts of essence, 25-30 parts of methyl salicylate, 600-650 parts of alcohol, and 282-355 parts of purified water. For the cannabidiol liquid preparation with the anti-anxiety effect and the preparation method of the cannabidiol liquid preparation, the anti-anxiety pharmacological action of cannabidiol is fully utilized, meanwhile, the plurality of traditional Chinese medicinal materials are mixed, so that the mind can be effectively soothed, and the anxiety can be alleviated.