Composite soothing essential oil and use method thereof

Abstract

The invention discloses composite soothing essential oil and a use method thereof, and belongs to the technical field of essential oil blending and application. The compound soothing essential oil is characterized in that an essential oil mixed solution comprises the following single essential oil in parts by volume: 5-20 parts of holly, 5-20 parts of peppermint, 5-20 parts of cananga odorata, 5-10 parts of camphor tree, 5-10 parts of German chamomile, 1-5 parts of blue wormwood, 1-5 parts of permanent flower, 1-5 parts of sweet-scented osmanthus and 1-5 parts of yarrow. According to the compound soothing essential oil disclosed by the invention, various single plant essential oil with good effects of relieving anxiety mood and tranquilizing and allaying excitement is organically blended and combined according to an active component and aroma compatibility principle, so that the compound soothing essential oil is prepared. All the components of the prepared compound soothing essential oil have a synergistic effect, and the compound soothing essential oil has good effects in the aspects of improving anxiety, regulating decompression, eliminating anxiety, calming and resisting depression. The composition is simple and convenient to use and obvious in effect, has an obvious anti-anxiety effect through sniffing intake, can relieve anxiety-like behaviors of mice, and provides a new effective method for preventing and relieving anxiety disorder.

Description

Technical Field

[0001] The invention belongs to the technical field of essential oil preparation and application, and relates to a compound essential oil, in particular to a compound soothing essential oil and a use method thereof. Background Art

[0002] Anxiety is a common emotional disorder. Individuals with anxiety disorders often experience nervous and stressed psychological reactions and present with symptoms such as palpitations, sweating, and insomnia. At present, modern medical interventions for anxiety disorders mainly include drug intervention and psychological adjustment. However, given that most people find it difficult to adapt to the venue and methods of psychotherapy and are unwilling to face up to their psychological symptoms, modern medicine urgently needs to explore a method that can not only encourage patients to actively accept appropriate psychotherapy, but also be gentle, with mild side effects or even no side effects, to regulate emotions and feelings to relieve anxiety.

[0003] Before the 20th century, people mainly used drugs with sedative and hypnotic effects to deal with anxiety disorders, such as bromine and chloral hydrate. These drugs have the disadvantages of significant sedative effects and obvious side effects. Later, pentobarbital drugs, benzodiazepine drugs, serotonin reuptake inhibitors and other drugs that were widely used showed strong adverse reactions, high dependence, and slow effect in subsequent clinical practice.

[0004] The sense of smell is a key sense of the human body. It can distinguish and process gas molecules with odors, and plays a role in assisting identification, warning of danger, affecting appetite and mood. It is one of the most primitive sensory functions of human beings. The sense of smell has a significant impact on emotions. Good smells can make people feel happy, and vice versa. Aromatherapy is an effective way to regulate the human body's condition with the help of volatile components extracted from aromatic plants. In modern times, essential oils are the most commonly used and common when using aromatic plants. Plant essential oils are organically blended and combined, and many known essential oil categories with good efficacy are used. The compound formula essential oils "created" according to the compatibility principle are more therapeutic and effective.

[0005] Therefore, in view of the urgent need to relieve anxiety disorders and the principle and good effect of essential oils in relieving anxiety, it is urgent to design a composite soothing essential oil with good stability and compliance that can effectively relieve anxiety and further broaden its application field. Summary of the invention

[0006] In order to solve the problems existing in the background technology, the purpose of the present invention is to provide a composite soothing essential oil with stability and compliance, which can effectively relieve anxiety and a preparation method thereof.

[0007] In order to achieve the above object, the technical solution of the present invention is as follows:

[0008] A composite soothing essential oil comprises an essential oil mixture, wherein the essential oil mixture comprises the following components by volume: 5-20 parts of wintergreen essential oil, 5-20 parts of peppermint essential oil, 5-20 parts of ylang-ylang essential oil, 5-10 parts of camphor tree essential oil, 5-10 parts of German chamomile essential oil, 1-5 parts of blue tansy essential oil, 1-5 parts of helichrysum essential oil, 1-5 parts of osmanthus essential oil, and 1-5 parts of yarrow essential oil.

[0009] Preferably, the chamomile essential oil and blue tansy essential oil are prepared by steam distillation, and the preparation comprises the following steps: placing chamomile or blue tansy and distilled water (1:3, w / v) in a flask, heating for 3 hours to generate steam, collecting the water and essential oil mixture, and centrifuging at 10,000g, 4°C for 10 minutes to obtain chrysanthemum essential oil.

[0010] Another technical purpose of the present invention is to provide an application of a compound essential oil in anxiety relief products, which is inhaled by sniffing, and specifically includes the following steps: in a closed space, the compound soothing essential oil is placed at a distance of 0.1 to 1 m from the human nostrils for 0.5 to 2 hours.

[0011] Preferably, the composite soothing essential oil is diluted with fractionated coconut oil before use, so that the volume concentration of the composite soothing essential oil in the product is 1-10% (v / v).

[0012] Preferably, the composite soothing essential oil is diluted with fractionated coconut oil before use until its concentration in the product reaches 2-6%.

[0013] Preferably, the frequency of use of the composite soothing essential oil is once per day.

[0014] Compared with the existing detection technology, the beneficial effects of the present invention are embodied in:

[0015] 1. The composite soothing essential oil of the present invention utilizes a plurality of plant essential oils, including holly, camphor, peppermint, German chamomile, blue tansy, helichrysum, ylang ylang, osmanthus and yarrow, which have good anxiety-relieving and calming effects, to be organically formulated and combined according to the active ingredients and aroma compatibility principles, and has a synergistic effect, and has good effects in improving anxiety, regulating and relieving pressure, eliminating restlessness, and calming and anti-depression.

[0016] 2. The composite soothing essential oil of the present invention is easy to use and has obvious effects. It has obvious anti-anxiety effects when inhaled by sniffing, and can relieve the anxiety-like behavior of test mice, providing a new and effective method for preventing and treating anxiety disorders. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a GC-MS characterization diagram of the composite soothing essential oil sample of the present invention;

[0018] Figure 2 This is a bar graph of the results of the sodium pentobarbital sleep test on mice in Example 3-1 of the present invention, wherein A latency is: the time it takes to fall asleep, that is, how long it takes to fall asleep; B sleep duration: refers to how long the sleep lasts;

[0019] Figure 3 This is a bar graph of the moving distance, central area time and average speed of mice treated with soothing essential oil in the open field test of mice in Example 3-2 of the present invention. In Figure C, ns means there is no statistically significant difference;

[0020] Figure 4 This is a bar graph of the experimental results of the time and number of times that mice entered the open and closed arms in the elevated plus maze experiment in Example 3-3 of the present invention;

[0021] Figure 5 This is a bar graph of the levels of serotonin, dopamine, norepinephrine and corticosterone in mice in the detection of neurotransmitters in serum in Example 3-4 of the present invention. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Example 1

[0024] A composite soothing essential oil comprises the following components by volume: 15 parts of wintergreen essential oil, 15 parts of peppermint essential oil, 5 parts of ylang ylang essential oil, 5 parts of camphor tree essential oil, 5 parts of German chamomile essential oil, 5 parts of blue tansy essential oil, 5 parts of helichrysum essential oil, 5 parts of osmanthus essential oil and 5 parts of yarrow essential oil.

[0025] The chamomile essential oil and blue tansy essential oil are prepared by steam distillation: chamomile or blue tansy and distilled water (1:3, w / v) are placed in a flask, heated for 3 hours to generate steam, the water and essential oil mixture is collected, and centrifuged at 10,000g, 4°C for 10 minutes to obtain chrysanthemum essential oil.

[0026] Example 2

[0027] A composite soothing essential oil comprises the following components by volume: 20 parts of wintergreen essential oil, 20 parts of peppermint essential oil, 15 parts of ylang ylang essential oil, 10 parts of camphor tree essential oil, 10 parts of German chamomile essential oil, 5 parts of blue tansy essential oil, 5 parts of helichrysum essential oil, 1 part of osmanthus essential oil and 5 parts of yarrow essential oil.

[0028] The preparation method of the chamomile and blue tansy essential oils is the same as that of Example 1-1.

[0029] Example 3

[0030] A composite soothing essential oil comprises the following components by volume: 5 parts of wintergreen essential oil, 5 parts of peppermint essential oil, 20 parts of ylang ylang essential oil, 10 parts of camphor tree essential oil, 8 parts of German chamomile essential oil, 1 part of blue tansy essential oil, 1 part of helichrysum essential oil, 2 parts of osmanthus essential oil and 1 part of yarrow essential oil.

[0031] The preparation method of the chamomile and blue tansy essential oils is the same as that of Example 1-1.

[0032] Comparative Example 1

[0033] The difference from Example 1 is that the chamomile essential oil used is a commercially available essential oil formula, and other configurations are the same.

[0034] Comparative Example 2

[0035] The difference from Example 1 is that the blue tansy essential oil used is a commercially available essential oil formula, and other configurations are the same.

[0036] Comparative Example 3

[0037] The difference from Example 1 is that the chamomile essential oil and blue tansy essential oil used are both commercially available essential oil formulas, and other configurations are the same.

[0038] Experimental Example 1

[0039] Take the composite soothing essential oil sample in Example 1 and use GC-MS to analyze the components of the soothing essential oil:

[0040] The conditions for GC-MS analysis of soothing essential oils are:

[0041] Shimadzu GC-MS-QP2020 NX-single quadrupole gas chromatograph-mass spectrometer, chromatographic column Agilent-DB-5ms, 30m*0.25mm*0.25μm, column box initial temperature 50℃, initial time 4min, heating rate 5℃ / min, end temperature 260℃, end time 10min, total flow rate 1mL / min, no split. Ion source temperature 230℃, interface temperature 280℃, mass number detection range 29-500. GC-MS analysis test characterization results are attached Figure 1 The chemical composition of the composite soothing essential oil samples is shown in Table 1:

[0042] Table 1 Chemical composition of the composite soothing essential oil samples

[0043]

[0044]

[0045]

[0046] It can be seen from the chemical composition of the above-mentioned composite soothing essential oil sample that the soothing essential oil of the present invention comprises 54 compounds including methyl salicylate cyclohexanone, dextrorotatory camphor, eucalyptol, D-limonene, 5-methyl 1-2-(1-methyl) cyclohexanone, linalool, methyl benzoate, etc., including phenyl esters, alcohols, terpenes, etc., and has a complex chemical composition, which has the effects of improving anxiety, regulating and relieving stress, eliminating uneasiness, and calming and anti-depression.

[0047] Experimental Example 2

[0048] The anxiety model was established by using Vital River ICR male mice (five weeks old) with the method of timed water supply training, and the relief effect on anxious mice was detected after different interventions.

[0049] ICR male mice of five weeks old and weighing 24-26g were selected. The ICR mice were kept in the same breeding environment with room temperature (20±2)℃, relative humidity 50%-65%, and good ventilation. They were fed with standard mouse feed and free food and water intake for one week of adaptive feeding. The mice in the model group were raised individually and trained to drink water at two fixed times every day at 9:00-9:10 and 21:00-21:10 in the first 7 days. After the scheduled water supply training, empty bottle stimulation began. Water was randomly supplied at one of the two fixed times, and only an empty bottle without water was provided to the mice at the other predetermined water supply time. The random number table method was used to determine the randomness. The empty bottle stimulation lasted for 14 days to establish the anxiety mouse model.

[0050] Experimental Example 2-1

[0051] The anxious mice were randomly divided into model group, positive drug group (diazepam), soothing essential oil group, 5 mice in each group, and normal mice as blank control. Each group was treated as follows, the treatment time was 30 days, and the anti-anxiety effect was evaluated.

[0052] (1) Blank control group: intraperitoneal injection of normal saline;

[0053] (2) Model group: intraperitoneal injection of normal saline;

[0054] (3) Positive drug group: diazepam (intraperitoneal injection of diazepam solution, dose of 1 mg / kg), once a day;

[0055] (4) Soothing essential oil group: Soothing essential oil treatment for 0.5 h, action concentration of 3%, action distance of 0.1 m, action frequency of once a day.

[0056] Experimental Example 2-2 Pentobarbital Sodium Sleep Experiment

[0057] After the mice were treated, each group of mice was intraperitoneally injected with sodium pentobarbital solution at a dose of 45 mg / kg. The time from the disappearance of the righting reflex of the mice (sleep latency) and the time from the disappearance of the righting reflex to the recovery of the mice (sleep duration) were observed and recorded. The results of the sleep test data processing and analysis of mice are shown in the attached Figure 2 As shown, the detection time of sleep latency and sleep duration is shown in Table 2.

[0058] Table 2 Detection time of sleep latency and sleep duration (min)

[0059] Group Latent time (min) Sleep duration (min) blank 3.40 47.20 Model 3.80 43.00 Positive 3.75 91.50 Example 1 Soothing essential oil 3.80 53.60

[0060] From the results in the figure, it can be seen that compared with the model group, the sleep duration of mice in the soothing essential oil treatment group was longer, indicating that soothing essential oil can effectively prolong the sleep time of anxious mice, but there was no significant difference in sleep latency between different groups.

[0061] Experimental Example 2-3 Mine Field Experiment

[0062] After the mice were treated, an open field test was performed. Each group of mice was placed in a mouse box, and then a behavioral recorder was used to capture the mouse's movements, and the time, average speed, and total distance the mice passed through the central area within 5 minutes were recorded. The test results of the moving distance, central area time, and average speed of the mice after soothing essential oil treatment are shown in the attached figure. Figure 3 The statistical data are shown in Table 3.

[0063] Table 3 Moving distance, central area time and average speed of mice after soothing essential oil treatment

[0064]

[0065]

[0066] Statistical data showed that the activity frequency and rate of mice treated with soothing essential oils were significantly improved compared with the model group, reflecting that soothing essential oils can relieve the anxiety level of mice, making them more active and increasing their exercise frequency.

[0067] Experimental Example 2-4 Elevated plus maze test

[0068] The anti-anxiety effect of the soothing essential oils prepared in each embodiment and comparative example was evaluated by using an elevated plus maze test, as follows:

[0069] After the mice were treated, they were subjected to the high-altitude plus maze test. Each group of mice was placed on the central platform, facing one of the open arms, and allowed to freely explore the entire maze. The behavioral parameters of the mice were recorded for 5 min, including: (1) the time spent in the open arms and closed arms (OT and CT); (2) the number of entries into the open arms and closed arms (OE and CE).

[0070] The percentage of times entering the open arms: OE% = OE / (OE+CE) × 100% and the percentage of time entering the open arms: OT% = OT / (OT+CT) × 100% were calculated as anxiety parameters. The results of the behavioral test data processing and analysis of mice are shown in the attached Figure 4 , statistical data are shown in Table 4:

[0071] Table 4 The time and number of times mice entered the open arms (%)

[0072]

[0073] The statistical data show that the percentage of open arm retention time and the percentage of open arm entry times of the soothing essential oil group prepared in Example 1 are both increased compared with those of the model group, indicating that the soothing essential oil has the effect of relieving anxiety in mice. However, the products in Comparative Examples 1-3, due to the change in the source of chrysanthemum essential oil, were tested, and the percentage of open arm retention time and the percentage of open arm entry times were not significantly improved compared with the model group, which shows that the preparation method of chrysanthemum essential oil is a key step. If a commercially available chrysanthemum essential oil is used for compounding, the effect of the final product is greatly reduced due to the different composition of the active ingredients contained in the essential oil.

[0074] Example 2-5 Detection of neurotransmitters in serum

[0075] After the mice were treated, blood was collected to separate serum, and the levels of 5-HT, dopamine, norepinephrine, and corticosterone were detected using Elisa kits. Figure 5 The statistical data are shown in Table 5.

[0076] Table 5 Neurotransmitter levels in mice after essential oil inhalation

[0077]

[0078] Statistical data show that soothing essential oil treatment can significantly increase the expression level of dopamine in serum, further proving that soothing essential oil has a good effect on relieving anxiety.

[0079] What is described above is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A composite soothing essential oil, characterized in that: The invention comprises an essential oil mixture, which comprises the following components by volume: 5-20 parts of wintergreen essential oil, 5-20 parts of peppermint essential oil, 5-20 parts of ylang-ylang essential oil, 5-10 parts of camphor tree essential oil, 5-10 parts of German chamomile essential oil, 1-5 parts of blue tansy essential oil, 1-5 parts of helichrysum essential oil, 1-5 parts of osmanthus essential oil, and 1-5 parts of yarrow essential oil.

2. A composite soothing essential oil according to claim 1, characterized in that: The chamomile essential oil and the blue tansy essential oil are prepared by steam distillation.

3. A composite soothing essential oil according to claim 2, characterized in that The method for preparing chamomile essential oil or blue tansy essential oil by steam distillation comprises the following steps: adding chamomile or blue tansy and distilled water into a flask at a mass concentration of 1:3, heating for 3 hours to generate steam, and collecting a mixture of water and essential oil; and then centrifuging at 4°C, 10,000 g, for 10 minutes to prepare the corresponding chrysanthemum essential oil of chamomile or blue tansy.

4. Application of a composite soothing essential oil according to claim 1 in anxiety relieving products.

5. The use of a composite soothing essential oil in anxiety relieving products according to claim 4, characterized in that: The volume concentration of the composite soothing essential oil in the product is 1-10%.

6. The use of a composite soothing essential oil in anxiety relieving products according to claim 5, characterized in that: The composite soothing essential oil is diluted with fractionated coconut oil before use until the volume concentration of the composite soothing essential oil in the product is 1-10%.

7. The use of a composite soothing essential oil according to claim 6 in anxiety relieving products, characterized in that: The composite soothing essential oil is diluted with fractionated coconut oil before use until its volume concentration in the product reaches 2-6%.

8. The use of a composite soothing essential oil according to any one of claims 4 to 7 in anxiety relieving products, characterized in that: The composite soothing essential oil is applied by inhalation.

9. The use of a composite soothing essential oil according to claim 8 in anxiety relieving products, characterized in that: In a closed space, place the compound soothing essential oil at a distance of 0.1 to 1 m from the nostrils of the ingestor for 0.5 to 2 hours.

10. The use of a composite soothing essential oil in anxiety relieving products according to claim 8, characterized in that: The frequency of use of the composite soothing essential oil is once per day.

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