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A method for extracting astaxanthin from Haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin is applied in the fields of biology and chemical technology, which can solve the problems of high-pressure homogenizers wearing fast, increasing production costs, and achieving the effects of less environmental pollution, low production costs, and less impurities.

Active Publication Date: 2016-02-10
QINGDAO KEHAI BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the high-pressure homogenization method has a high wall-breaking rate among various mechanical wall-breaking methods and can realize industrial production. However, the high-pressure homogenizer wears out quickly, which significantly increases the production cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step 1: High-pressure extraction to break the algal wall

[0029] Algae body wall breaking: Weigh 10g algae body, add 100mL soybean oil to the algae body, pump it into a high-pressure container at a flow rate of 345mL / min, and carry out wall breaking treatment at a pressure of 100Mpa and a temperature of 4°C. centrifuged to obtain astaxanthin extract and algae residue;

[0030] Step 2: Rapid separation of astaxanthin

[0031] Extraction: Mix astaxanthin extract, algae residue and silica gel at a weight ratio of 2:1, then add 5mL of ethanol and 15mL of ethyl acetate, stir and extract for 1 hour, then put the extraction mixture into a chromatography column;

[0032] Elution: elute with ethanol, collect the red effluent;

[0033] Concentration: The red effluent was distilled under reduced pressure to obtain a dark red astaxanthin oily liquid.

[0034] Step 3: Crude extraction of astaxanthin oily liquid

[0035] Saponification: Mix astaxanthin oily liquid with 0.06mol / L...

Embodiment 2

[0041] Step 1: High-pressure extraction to break the algal wall

[0042] Algae body wall breaking: Weigh 10g algae body, add 100mL soybean oil to the algae body, pump it into a high-pressure container at a flow rate of 345mL / min, and carry out wall breaking treatment at a pressure of 200Mpa and a temperature of 4°C. centrifuged to obtain astaxanthin extract and algae residue;

[0043] Step 2: Rapid separation of astaxanthin

[0044] Extraction: Mix astaxanthin extract, algae residue and silica gel at a weight ratio of 3:1, then add 8mL of ethanol and 18mL of ethyl acetate, stir and extract for 2 hours, then put the extraction mixture into a chromatography column;

[0045] Elution: elute with ethanol, collect the red effluent;

[0046] Concentration: The red effluent was distilled under reduced pressure to obtain a dark red astaxanthin oily liquid.

[0047] Step 3: Crude extraction of astaxanthin oily liquid

[0048] Saponification: Mix astaxanthin oily liquid with 0.06mol / L ...

Embodiment 3

[0054] Step 1: High-pressure extraction to break the algal wall

[0055] Algae body wall breaking: Weigh 10g algae body, add 100mL soybean oil to the algae body, pump it into a high-pressure container at a flow rate of 345mL / min, and carry out wall breaking treatment at a pressure of 300Mpa and a temperature of 4°C. centrifuged to obtain astaxanthin extract and algae residue;

[0056] Step 2: Rapid separation of astaxanthin

[0057] Extraction: Mix astaxanthin extract, algae residue and silica gel at a weight ratio of 3:1, then add 10mL of ethanol and 20mL of ethyl acetate, stir and extract for 3 hours, then put the extraction mixture into a chromatography column;

[0058] Elution: elute with ethanol, collect the red effluent;

[0059] Concentration: The red effluent was distilled under reduced pressure to obtain a dark red astaxanthin oily liquid.

[0060] Step 3: Crude extraction of astaxanthin oily liquid

[0061] Saponification: Mix astaxanthin oily liquid with 0.06mol...

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Abstract

The invention provides a method for extracting astaxanthin from haematococcus pluvialis, and belongs to the technical fields of bioprocess technology and chemical technology. The method comprises the following steps: completing the selection of raw materials through abnormal growth of cells of the selected haematococcus pluvialis, dilution of high-density algae cells and light induction, carrying out wall-breaking treatment on frond by using an ultrahigh pressure extractive technology, then extracting, eluting, concentrating, saponifying and centrifuging, thus obtaining the astaxanthin. The method has the advantages that the consumption of an organic solvent is little, an extraction agent can be circularly utilized, the environmental pollution is little, the production cost is low, the recovery rate is high, few impurities exist, the possibility of oxidative degradation of the astaxanthin is reduced, and the extraction quality of the astaxanthin is improved.

Description

technical field [0001] The invention relates to a method for extracting astaxanthin, in particular to a method for extracting astaxanthin from Haematococcus pluvialis, and belongs to the technical field of biological and chemical processes. Background technique [0002] Astaxanthin belongs to carotenoids, bright red, is a high-quality pigment and super antioxidant, a carotenoid with a structure of o-hydroxy ketone, its chemical name is 3,3-dihydroxy-4,4-di Keto-β, β′-carotene, pure astaxanthin is a dark reddish-brown crystal with a melting point of 224°C, insoluble in water but soluble in most organic solvents. Astaxanthin has strong anti-oxidation and ability to scavenge free radicals. Clinical and animal experiments have shown that astaxanthin has good anti-inflammatory, anti-tumor, anti-aging, skin protection, enhanced immunity, prevention of cardiovascular diseases, Maintain eye health, improve animal fertility and many other important biological functions. In addition...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
Inventor 李悦明管斌李元广徐建春夏修峦王道会
Owner QINGDAO KEHAI BIOLOGICAL
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