Chlorella Chlorella sp.HQ culture method and application in water purification and oil production

A technology of water quality purification and cultivation methods, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as water bloom outbreaks, achieve protection of water resources, reduce cultivation costs, and reduce costs Effect

Inactive Publication Date: 2014-02-12
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Excessive content of nutrients such as nitrogen and phospho

Method used

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  • Chlorella Chlorella sp.HQ culture method and application in water purification and oil production
  • Chlorella Chlorella sp.HQ culture method and application in water purification and oil production
  • Chlorella Chlorella sp.HQ culture method and application in water purification and oil production

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Experimental program
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Effect test

Embodiment 1

[0024] Chlorella HQ was inoculated in the secondary effluent of Beijing Qinghe Sewage Treatment Plant with an initial inoculation density of 5×10 4 pcs mL -1 , the light-to-dark ratio is 12:12, the temperature is 20°C, and the light intensity is 40μmol·(m 2 s) -1 The growth curve, biomass, oil production and TAG production of Chlorella were measured after culturing for about 14 days. Centrifuge at 4500rpm for 15min after the culture is over to obtain algae.

[0025] The result is as figure 1 As shown, the maximum density of chlorella growing in the secondary effluent of Qinghe sewage treatment plant can reach 10 6 pcs mL -1 , the biomass can be as high as 98mg·L -1 , the oil production can be as high as 41.6mg·L -1 , TAG production can be as high as 18.7mg·L -1 .

[0026] Chlorella HQ was inoculated in the secondary effluent of Beijing Xiaojiahe Wastewater Treatment Plant with an initial inoculation density of 2×10 5 pcs mL -1 , the light-to-dark ratio is 13:11, the...

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Abstract

The invention discloses a chlorella Chlorella sp.HQ culture method and application in water purification and oil production, and belongs to the technical field of production of single cell oil and biodiesel. The oil-producing freshwater chlorella is named chlorella HQ and is stored with registration number as CGMCC No.7601. The culture method comprises the following steps of: inoculating the chlorella HQ strain in a logarithmic phase into secondary effluent of municipal sewage; controlling the chlorella inoculums density to be 5*10<4> to 5*10<5> mL<-1>, light-dark ratio to be (12:12)-(14:10), illumination intensity to be 40 to 60 micromole.(m2.s)-1, temperature to be 20 to 30 DEG C, and pH (Potential of Hydrogen) to be 6 to 9; culturing for 10 to 14 days under the conditions above; and separating and obtaining frond as well as collecting the purified water. The application of the chlorella HQ in water purification and oil production is characterized in that the secondary effluent of municipal sewage is used as a culture medium, and the chlorella HQ enables the pollutant in the secondary effluent to be continuously converted and proliferated in order to accumulate single cell oil, simultaneously the water is deeply purified, and the water resource is protected; and therefore, the culture method brings double benefit, and is environmentally-friendly, low in cost and beneficial for the industrial application of chlorella HQ by large-scale culture.

Description

technical field [0001] The invention belongs to the technical field of single-cell oil and biodiesel production, and in particular relates to a cultivation method of Chlorella spHQ and its application in water purification and oil production. Background technique [0002] Energy is the source of power to promote social development, of which fossil energy occupies a dominant position. However, the rapid development of human society has accelerated the depletion of coal, oil and other fossil energy sources for its survival. In addition, the massive burning of fossil energy will also cause a series of environmental problems, such as CO in the atmosphere 2 Increased concentrations, global climate change, etc. Therefore, under the circumstances of energy crisis and environmental problems becoming more and more prominent, the development of clean energy has become inevitable. In recent years, biofuels, as the only alternative to fossil fuels, have become the fastest growing and...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C02F3/32C12R1/89
Inventor 洪喻张巧
Owner BEIJING FORESTRY UNIVERSITY
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