Preparation and application of fused oral interferon for fowl

An interferon and fusion technology, applied in the field of biopharmaceuticals, can solve the problems of easy contamination of protein products, complex processing and purification, and harsh conditions, and achieve the effects of enhancing the body's immunity, good biological safety, and not easy to contaminate

Active Publication Date: 2012-11-14
LUOYANG RUILAI BIOLOGICAL ENG CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The prokaryotic expression system cannot perform protein transcription and post-translational modification functions, and the expression products are mostly insoluble intracellular inclusion bodies, the yield is low, and complex processing and purificati

Method used

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  • Preparation and application of fused oral interferon for fowl
  • Preparation and application of fused oral interferon for fowl
  • Preparation and application of fused oral interferon for fowl

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Embodiment Construction

[0034] The basic operation techniques of genetic engineering used in the present invention are carried out according to the method provided in the "Refined Molecular Biology Experiment Guide (Fourth Edition)" published by Science Press in 2005, or according to the instructions for the purchased reagents and products.

[0035] Embodiments of the invention relate to the following materials:

[0036] The pMD18-T cloning vector and pBI-eukaryotic expression vector were purchased from Dalian Bao Biological Co., Ltd.; Escherichia coli competent was purchased from Dalian Bao Biological Co., Ltd.; restriction enzymes Xba , Bam H , SacT4 DNA ligase was purchased from Dalian Bao Biological Co., Ltd.; Trizol reagent was purchased from Invitrogen Company of the United States; PCR cleaning kit and gel recovery kit were purchased from Axygene Company of the United States; specific primers were synthesized by Shanghai Synthesized by Sangon Biological Co., Ltd.;

[0037] The preparati...

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Abstract

Preparation and application of a fused oral interferon expression frond for fowl are disclosed. On the basis of the conserved sequences of fowl interferon alpha and gamma genes, the interferon alpha gene having great antiviral effect and the interferon gamma gene capable of improving the immunity of the organism and enhancing the constitution of the organism are fused for expression, so that the expressed alpha+gamma fused interferon has the synergetic functions of the two. In the mean time, a dunaliella bioreactor is used as an expression system; apart from that the system is capable of expressing exogenous genes, own advantages such as rich nutrition, nontoxicity, harmlessness and the like of dunaliella are also utilized; consequently, a gene algae plant for multiple purposes is obtained; the fused oral interferon expression frond for fowl prepared from the genetically modified algae plant can be applied to the fields such as prevention or treatment of fowl viroses, fowl breeding, related feed processing industry and the like; and the fused oral interferon for fowl is good in effect, green and environment-friendly, and also low in cost.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to the preparation and application of a fusion type interferon for poultry. Background technique [0002] At present, the most harmful and difficult-to-control diseases to my country's poultry breeding industry are infectious viral diseases. For such diseases of poultry, there have been no effective treatment methods and means, among which vaccination is the main measure and key link to prevent the outbreak and prevalence of poultry viral diseases. However, with the failure of vaccine prevention or untimely vaccination, some viral diseases often break out in poultry farming, and the infectivity and mortality are extremely high, and some can reach 100%. Once a viral disease breaks out, biologics such as interferon are currently the first choice for treatment on the market. However, the interferon currently used in the market is mainly monovalent, and most of them are injection-bas...

Claims

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Application Information

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IPC IPC(8): C12N15/79C12N1/13A61K38/21A61K36/05A61P31/12A23K1/16C12R1/89
Inventor 冯书营王艳鸽王臣李光大李爱芳祁松楠
Owner LUOYANG RUILAI BIOLOGICAL ENG CO LTD
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