Method for extracting phycoerythrin and gelose synchronously from gum-contained varek such as gardon asparagus

A technology of phycoerythrin and astragalus, applied in application, food preparation, food science and other directions, can solve the problems of inability to spend summer and winter, inability to accumulate biomass effectively, and achieve the effect of improving utilization rate

Inactive Publication Date: 2008-03-12
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this species cannot spend summer and winter in the Ye...

Method used

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  • Method for extracting phycoerythrin and gelose synchronously from gum-contained varek such as gardon asparagus
  • Method for extracting phycoerythrin and gelose synchronously from gum-contained varek such as gardon asparagus
  • Method for extracting phycoerythrin and gelose synchronously from gum-contained varek such as gardon asparagus

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Embodiment Construction

[0027] Below in conjunction with embodiment, the present invention is further described; Following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.

[0028] This embodiment only takes the typical gelatin-containing seaweed—Asparagus asparagus as an example. Other gelatin-containing seaweeds can extract the contained alginate and related proteins according to this method, and this embodiment will not describe them one by one.

[0029] 1. Extraction and purification method of phycoerythrin

[0030] (1). Weigh 55 g wet weight of fresh asparagus and chop it up, add a small amount of distilled water overnight at 4°C to swell and lyse the cells.

[0031] (2). Centrifuge at 10,000 rpm and 4° C. for 10 minutes, collect all the supernatant, and obtain 340 ml of crude extract.

[0032] (3). According to the formula R-PE=155.8OD 498.5 -40.0OD 614 -10.5OD 651 , calculated to contain 66.630mg phycoerythrin in t...

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Abstract

The present present invention relates to a method of simultaneously extracting phycoerythrin and agar from seaweeds such as asparagus, etc in seaweeds biological chemical field. The concrete operation process is that cells of the asparagus frond are crushed. A phycobiliprotein crude extract is obtained from water soluble extracts after ammoniym sulfate precipitation. The crude extract is extracted by Phenyl-Sepharose expansion columns and phycoerythrin products are obtained. Q-Sepharose iron exchange columns are used to purify the phycoerythrin products to obtain purified phycoerythrin products. Then, the left asparagus residues after the phycoerythrin extract are used to extract the agar. The present invention can simultaneously obtain the phycoerythrin and the agar from the asparagus. And the purified phycoerythrin purity is larger than 2.954, and the yield is 0.097mg per gram of fresh algae. The yield of the obtained agar is 2.31percent (the algae is wetly weighted), which is equivalent to the yield of an agar direct extraction method. Therefore, the utilization ratio of the asparagus can be greatly improved by adopting the method.

Description

technical field [0001] The invention relates to the technical field of seaweed biochemical industry, in particular to a method for simultaneously extracting phycoerythrin and agar from asparagus and other gelatin-containing seaweeds. Background technique [0002] Asparagus (Gracilaria Lemaneiformis) is an important cultivated species of Gracilaria, belonging to Rhodophyta, Rhodophyceae, Florideae, Gigartinales, Gracilaria Family (Gracilariaceae), Gracilaria (Gracilaria). Wild asparagus is mainly distributed in the intertidal zone along the coast of Shandong in my country. It has many branches and grows fast. The optimum growth temperature is 15-25°C. At present, asparagus has become the third largest cultivated seaweed after kelp and seaweed in my country. However, this species cannot spend summer and winter in the Yellow Sea and Bohai Sea area of ​​origin, so it cannot effectively accumulate biomass. For this reason, after realizing the cultivation of asparagus to the s...

Claims

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Application Information

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IPC IPC(8): A23L1/28A23L1/337A23L1/305A23L1/0532A23J1/00A23L29/256A23L33/185
Inventor 王广策张开岳牛建峰
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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