Method for generating macrogametocyte by somatic cell of inducing undaria pinnatifida gynecogenic juvenile sporophyte

A technology of female gametophyte and parthenogenesis is applied in the field of inducing parthenogenetic young sporophyte somatic cells of Wakame to produce female gametophytes, which can solve the problems of limited number of male and female gametophytes, difficulty in meeting the requirements of the number of female gametophytes, etc. Solve the effect of insufficient number of female gametophytes and simple operation

Inactive Publication Date: 2010-06-02
DALIAN FISHERIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, due to the limited number of male and female gametophytes after separation, especially the large number of male gametophytic cells, one cell can form multiple sperm vesicles, while the female gametophytic cells have a small number and only apical cells can form oocysts. Proliferation and culture, but the female gametophyte is still difficult to meet the quantity requirements of large-scale seed production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The male and female wakame gametophytes can be separated and separately preserved and cultivated according to the method for separating male and female in the prior art, and then carried out according to the following steps:

[0020] a. Take 10mg of preserved and cultured Wakame female gametophyte (wet weight), add 300ml of sterilized seawater, chop it into 190-210μm algae segments with a tissue mixer, and form female gametophyte body fluid, use a straw to culture female gametophyte body fluid at 1ml / time The dishes were moved to multiple petri dishes (diameter 9cm, the same below), added culture medium (sterilized seawater with nutrient salts added) and then moved to culture at a temperature of 20°C, a light intensity of 2000Lx, and a light time of 12 hours / day For 12 days, the culture medium was fully exchanged once every 3 days; the wet weight of the female gametophytes of Undaria pinnatae and the volume of added sterilized seawater can be adjusted according to the ra...

Embodiment 2

[0029] Other steps are all as embodiment 1, and the wakame female gametophyte that preserves culture as described in step a can obtain in the following manner:

[0030]f. During the breeding period of Undaria pinnatifida, take the mature spore leaves of algae cultivated on floating rafts, and put them into a beaker with sterilized seawater after being stimulated by drying in the shade. When it is observed that a large number of spores are released and the spore water is light brown, remove the spores Leaves, use a straw to get a small amount of spore water and move it to a petri dish added with culture solution (sterilized seawater with nutrient salt added). After 2 hours, it can be seen that round embryospores are attached to the bottom of the petri dish. Move the petri dish to a temperature of 20°C. Cultivate for 1 month under the conditions of ℃ and illuminance of 2000Lx, and exchange the culture medium once every 10 days;

[0031] After 2 days of culture, the undaria pinna...

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Abstract

The invention discloses a method for generating macrogametocytes by somatic cells of inducing undaria pinnatifida parthenogenesis juvenile sporophytes, which has easy operation, low cost and high maturation rate of the generated macrogametocytes and can provide a large quantity of the macrogametocytes for the large-scale seedling production of undaria pinnatifida. The method comprises the following steps of: cutting undaria pinnatifida macrogametocytes stored and cultured in a room into pieces and then culturing under the conditions of temperature suitable for the undaria pinnatifida macrogametocytes to grow to be mature, illumination and nutritive salt, wherein when the undaria pinnatifida macrogametocytes grow to be mature, form oocysts in large quantities and ovulate with ovums, a small quantity of the fertilized ovums are germinated to seedlings through parthenogenesis because unfertilized ovums are died by being unfertilized by spermatozoa; and when the somatic cells of the parthenogenesis seedlings are grown to 0.4-0.6 cm, adopting a rhizoid cutting method to enable fronds to lose polarity and constraint on the somatic cells, and inducing the somatic cells of the seedlings to generate the macrogametocytes.

Description

Technical field: [0001] The invention relates to a method for producing female gametophytes of wakame, in particular to an induced wakame that is simple to operate, low in cost, has a high maturation rate of the produced female gametophytes, and can provide a large amount of female gametophytes for the large-scale production of undaria seedlings A method in which parthenogenetic young sporophyte somatic cells produce female gametophytes. Background technique: [0002] Wakame (Undaria pinnatifida) belongs to Phaeophyta, Brownia, Laminaria, Pteraceae, and Undaria genus. It belongs to euthermic algae. It is delicious and nutritious. , sugar and vitamins, because the cellulose content of wakame is only about 3% of the dry weight, it is easy to be digested by the human body, and it is a healthy food popular among consumers. [0003] The large algae of Undaria pinnata are sporophytes, and seedlings appear in natural sea areas every autumn, and grow into 1-2m algae in winter and s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 张泽宇李晓丽曹淑青由学策
Owner DALIAN FISHERIES UNIVERSITY
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