Construction and transformation of chamomile farnesyl diphosphate synthase (FPS) gene eukaryotic expression vector

A technology of nicyl pyrophosphate synthase and eukaryotic expression vector, which is applied in the direction of genetic engineering, plant gene improvement, and the introduction of foreign genetic material using vectors, etc., to achieve the effect of increasing production and making great contributions

Inactive Publication Date: 2012-12-12
ANHUI AGRICULTURAL UNIVERSITY
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research on the enzymes and genes in the volatile o

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction and transformation of chamomile farnesyl diphosphate synthase (FPS) gene eukaryotic expression vector
  • Construction and transformation of chamomile farnesyl diphosphate synthase (FPS) gene eukaryotic expression vector
  • Construction and transformation of chamomile farnesyl diphosphate synthase (FPS) gene eukaryotic expression vector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0048] 1. Materials and methods

[0049] 1.1 Experimental materials

[0050] 1.1.1 Plant materials: chamomile flowers (collected from the Nongcui Garden of Anhui Agricultural University), aseptic seedlings of Yunyan 85 tobacco (provided by the tissue culture room of the College of Life Sciences, Anhui Agricultural University).

[0051] 1.1.2 Strains and plasmids: Escherichia coli DH5a competent cells were purchased from TAKARA Company, Agrobacterium EHA105 and pBI121 vectors were donated by Mr. Jiang Haiyang, School of Life Sciences, Anhui Agricultural University, pEASY-T4 Zero Cloning Vector and Trans1-T1 competent cells were purchased From Beijing Quanshijin Biotechnology Co., Ltd.

[0052] 1.1.3 Main biological reagents: Restriction enzymes SacI, BamHI, T4DNA ligase, rtaq were purchased from TAKARA company, TransStart Taq DNA Polymerase was purchased from Beijing Quanshijin Biotechnology Co., Ltd., RNAiso plus, DNA molecular weight standard, DNA gel recovery kit and plasmid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a construction and transformation method of a chamomile farnesyl diphosphate synthase (FPS) gene eukaryotic expression vector, comprising the steps of: extracting an RNA (ribonucleic acid) from chamomile petals, and inversely transcribing the RNA into cDNA (complementary deoxyribonucleic acid) with total chamomile RNAs as a template; carrying out PCR (Polymerase Chain Reaction) amplification using a cDNA as a template to obtain a target fragment; connecting the target fragment with a T4 ligase; transforming a connection product to an escherichia coli DH5 alpha competent cell to obtain an eukaryotic expression vector PBI121/FPS; then introducing a recombinant expression vector to agrobacterium tumefaciens EHA105; and infecting the plant Yunyan85 by using the agrobacterium tumefaciens EHA105 to finally obtain a resistant tobacco plant. An FPS gene is cloned by using a gene engineering technology, the callus tissues of the plant are infected by virtue of the agrobacterium tumefaciens, and the construction and transformation method lays a foundation for improvement of chamazulene yields through expression of the FPS gene in a plant frond.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a chamomile farnesyl pyrophosphate synthase gene cloning, eukaryotic expression and application. Background technique [0002] Chamomile (Matricaria chamomilla L.) is an annual herb of the genus Matricaria in the Asteraceae family. The whole plant is fragrant and contains aromatic volatile oil. Chamomile is anti-inflammatory, anti-fungal, antispasmodic [1-3] , Improve the symptoms of lung cancer patients and so on. Chamomile originates in Europe and was introduced into my country at the beginning of this century. It is rarely cultivated in Beijing, Shanghai, Jiangsu, Hunan and other places. Chamomile contains a variety of chemical components: volatile oil, flavonoid glycosides, guaiazolactones, coumarin, polysaccharides, organic acids and other components. Chamomile volatile oil is the most important active ingredient in inflorescences. Its volatile oil is widely used in food, bev...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/66C12N15/54C12N15/82
Inventor 袁艺杨秀梅邰玉玲
Owner ANHUI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap