Grading method of humic acid large-pore-size resin in frond

A classification method, technology of humic acid, applied in the direction of organic chemistry, etc.

Inactive Publication Date: 2015-12-16
CHINESE RES ACAD OF ENVIRONMENTAL SCI
View PDF3 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the fractionation of humic acid in algae

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1: Fractional extraction of humic acid in brown algae East China Sea sample:

[0060] In April 2014, samples of brown algae from the East China Sea were collected;

[0061] XAD-8 resin column; Sigma company;

[0062] Sodium hydroxide: analytically pure; hydrochloric acid: analytically pure; sodium pyrophosphate: analytically pure;

[0063] The specific operations of this embodiment are as follows:

[0064] Step a, algae body pretreatment: Take the air-dried algae body, remove impurities, dry at room temperature, grind and sieve to obtain 5kg algae body powder;

[0065] Step b, algae Soxhlet extraction:

[0066] Put 5kg of algae powder in a Soxhlet extractor, and use ether as the extraction solution for Soxhlet extraction. During the extraction process, measure the absorbance of the extraction solution under ultraviolet-visible light with a wavelength of 220nm every 5 hours. When the absorbance is greater than or equal to When the absorbance is less t...

Embodiment 2

[0110] Embodiment 2: This embodiment discloses a method for grading humic acid macroporous resins in algal bodies, and the method for grading and extracting comprises the following steps:

[0111] Step a, algae body pretreatment: take the air-dried algae body, remove the sundries, in 30-50 o Dry at C, and sieve after grinding to obtain the algae body powder;

[0112] Step b, Soxhlet extraction of algae body: place the algae body powder in a Soxhlet extractor, and use ether, acetone, 95% ethanol, and dioxane as the extracting solution to carry out extraction; during the extraction process, when When one of the extraction solutions is used for extraction, the absorbance of the extraction solution is measured under ultraviolet-visible light with a wavelength of 220nm every 5h. When the absorbance of the extraction solution is greater than or equal to 0.01, the extraction is continued. Process; when the measured absorbance is less than 0.01, replace the next extraction solution f...

Embodiment 3

[0131] Embodiment 3: The difference between this embodiment and Example 2 is that in step h in this embodiment, when washing the XAD-8 resin column with a sodium pyrophosphate buffer solution of a certain pH value, every 2- The 50ml effluent is measured with a specific wavelength of ultraviolet / visible wavelength. The ultraviolet / visible absorbance value of the effluent increases first and then decreases. When the ultraviolet / visible absorbance value of the effluent is less than 0.5% of the maximum absorbance value, the pH value is stopped. Rinse with sodium pyrophosphate buffer.

[0132] In step i of this embodiment, after the dialysis system of each dialysis bag was stirred for 12 hours, the content of total phosphorus and the content of chloride ions in deionized water in step i were respectively determined by ammonium molybdate spectrophotometry and silver nitrate method, if the total phosphorus content>0.01mg / L or silver chloride exists, replace the ultrapure water in the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a grading method of a humic acid large-pore-size resin in frond. The graded extraction method comprises the following steps: frond pretreatment, Soxhlet extraction of frond, calcium chloride treatment of frond, sodium carbonate treatment of frond, alkali solution extraction of frond, frond humic acid silicon removal, frond humic acid fulvic acid removal, subcomponent grading of frond humic acid, and desalinization and solidification of frond humic acid subcomponent. The method can extract humic acid from the brown alga organism and perform subcomponent separation by using an XAD-8 resin separation technique, thereby finally obtaining the graded frond humic acid subcomponent.

Description

Technical field [0001] The present invention involves the separation and extraction technology of humus acid, which specifically involves a large -scale diameter resin classification method in a algae body. Background technique [0002] Humicacid, also known as Humicacid, is a macromolecular organic mixture formed by the animals and plants and its residues through complex physics, chemistry, and biological processes. It exists in environmental media such as soil, water body, and sediment.Exic acid can be soluble in alkaline and neutral solutions, insoluble in acid.Exic acid contains a large amount of active functional groups such as carboxyl, phenol hydroxyl group, cymbal, amino, and cymbal groups, which has high reaction activity.For example, in a natural environment, humans can interact with toxic heavy metal ions and organic pollutants in the environment (such as heavy metals, pesticides, PPCPS, PAHS, etc.) to form chemistry and biological stability soluble in water and insolu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08H99/00C07G99/00
Inventor 白英臣吴丰昌孙福红陈曲宋凡浩
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap