Process for preparing liquid seaweed fertilizer through enzymolysis and fermentation

A liquid fertilizer and preparation technology, applied in the direction of organic fertilizers, fertilizer mixtures, fertilization devices, etc., can solve the problems of refractory seaweed macromolecules, affecting the effect of seaweed fertilizers, single fermentation microbial strains, etc., to promote the growth of plants Effects of cell division, enlargement of vascular bundle cells, and improvement of aggregate structure

Active Publication Date: 2015-01-07
JIANGMEN ZHIHU IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many defects in the preparation of seaweed fertilizers by biological fermentation methods. One is that the fermentation microorganisms are single, and the enzymes produced are single, which makes it difficult to truly degrade the macromolecules of seaweeds, which affects the effect of seaweed fertilizers. The second is the biological fermentation method. In the preparation of sea

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1:

[0019] A process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, and the specific preparation steps are as follows:

[0020] (1) Kelp pretreatment: Rinse the kelp with clean water, remove impurities, and drain; cut the kelp into 3~5cm length with a knife; use 0.1%Ca(OH) with pH 6.5~7.0 2 The solution is to soak the Enteromorpha for 3 times, each time for 15 minutes, for desalting and color protection; drying at 85°C, finally grinding, and passing through a 40-mesh sieve;

[0021] (2) Ultrasonic enzymatic hydrolysis: add 8 times the mass of kelp water, adjust pH 6.8-7.2; add 3% glucoamylase and 2% non-starch polysaccharidase according to the mass ratio of kelp, ultrasonic-assisted enzymatic hydrolysis at 40-60℃ for 2h, ultrasonic Power 400w, frequency 25kHz;

[0022] (3) Fermentation broth preparation: Add 3.0% glucose, 2.0% yeast extract, 0.3% potassium dihydrogen phosphate, 0.02% MgSO to the enzymatic hydrolysis solution. 4 .7H 2 O, 0.01%C...

Example Embodiment

[0026] Example 2:

[0027] A process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, and the specific preparation steps are as follows:

[0028] (1) Pretreatment of Enteromorpha: Rinse Enteromorpha with clean water, remove impurities, and drain; Use a knife to cut Enteromorpha into 3~5cm length; Use 0.1%Ca(OH) pH6.5~7.0 2 The solution is to soak the Enteromorpha for 3 times, each time for 15 minutes, for desalting and color protection; drying at 85°C, finally grinding, and passing through a 40-mesh sieve;

[0029] (2) Ultrasonic enzymatic hydrolysis: Add 9 times the quality of Enteromorpha water and adjust the pH to 6.8-7.2; add 2% glucoamylase and 1% non-starch polysaccharidase according to the weight ratio of Enteromorpha, and ultrasonic-assisted enzymatic hydrolysis at 40-60°C for 4 hours , Ultrasonic power 400w, frequency 25kHz;

[0030] (3) Preparation of fermentation broth: add 5.0% glucose, 3.0% yeast extract, 0.5% potassium dihydrogen phosphate, 0.02%...

Example Embodiment

[0034] Example 3:

[0035] A process for preparing seaweed liquid fertilizer by enzymatic hydrolysis and fermentation, the specific preparation steps are as follows:

[0036] (1) Sargasso algae pretreatment: Rinse the Sargasso with clean water, remove impurities, and drain; use a knife to cut the Sargasso into 3~5cm lengths; use 0.1%Ca(OH) pH6.5~7.0 2 The solution is to soak the Enteromorpha for 3 times, 15min each time, to carry out desalting and color protection treatment; drying at 85℃, and finally grinding and passing through a 40-mesh sieve;

[0037] (2) Ultrasonic enzymatic hydrolysis: add 10 times the mass of Sargassum water to adjust pH 6.8~7.2; add 2.5% glucoamylase and 1.5% non-starch polysaccharidase according to the mass ratio of Sargassum, and ultrasonic assist enzymatic hydrolysis at 40~60℃ for 3h , Ultrasonic power 400w, frequency 25kHz;

[0038] (3) Fermentation broth preparation: add 4.0% glucose, 2.5% yeast extract, 0.4% potassium dihydrogen phosphate, 0.02% MgSO t...

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Abstract

The invention discloses a process for preparing a liquid seaweed fertilizer through enzymolysis and fermentation. The process disclosed by the invention can be used for generating a large quantity of soil beneficial bacteria and multiple special-effect metabolites by degrading macromolecular substances which are contained in seaweeds and difficult to utilize into micromolecular polysaccharide, monosaccharide and a nitrogen-containing compound in compound enzymolysis and multi-strain co-fermentation modes, thereby enhancing the biological activity of a seaweed fertilizer, preventing the destruction of strong base, high temperature and high pressure in the production process of the seaweed fertilizer and completely retaining abundant nutrient substances contained in the seaweeds. According to the process, the content of a product, namely alginic acid, is 40-60 g/L and is increased by more than 30% compared with that prepared from an enzymolysis method, an alkaline hydrolysis method, an acid hydrolysis method and a simple microbe fermentation method, and the effective viable count is more than or equal to 2 billion/mL; the product integrates natural nutrient components, antibiotic substances and plant hormones, combines the efficacy of three fertilizers, namely an organic fertilizer, a microbe fertilizer and a trace element fertilizer and has a wide application prospect.

Description

technical field [0001] The invention relates to a process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, which belongs to the field of fertilizer production. Background technique [0002] Seaweed is a rich source of marine organisms, widely used in food, industrial raw materials, and herbal medicines. Seaweed has been used in agriculture and livestock production for centuries. Seaweed fertilizer is a new type of pure natural, non-toxic and side-effect fertilizer. It contains rich non-N organic matter and a certain amount of amino acids, proteins and micronutrients, and also contains seaweed polysaccharides, alginic acid, and alginic acid unique to marine organisms. Unsaturated fatty acids and mineral elements such as zinc, bromine and iodine, which are rare in terrestrial plants, and rich in vitamin C and B 1 , B 2 , B 12 , D, E, K and other nutrients have the functions of increasing crop yield, improving quality and enhancing crop resistance to...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G3/04C05G3/80
CPCC05D9/02C05G3/00C05G3/80C05F11/00C05F11/08
Inventor 董书阁侯文杰侯文燕董静静段智岗代书强
Owner JIANGMEN ZHIHU IND CO LTD
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