Process for preparing liquid seaweed fertilizer through enzymolysis and fermentation

A liquid fertilizer and preparation technology, applied in the direction of organic fertilizers, fertilizer mixtures, fertilization devices, etc., can solve the problems of refractory seaweed macromolecules, affecting the effect of seaweed fertilizers, single fermentation microbial strains, etc., to promote the growth of plants Effects of cell division, enlargement of vascular bundle cells, and improvement of aggregate structure

A liquid fertilizer and preparation technology, applied in the direction of organic fertilizers, fertilizer mixtures, fertilization devices, etc., can solve the problems of refractory seaweed macromolecules, affecting the effect of seaweed fertilizers, single fermentation microbial strains, etc., to promote the growth of plants Effects of cell division, enlargement of vascular bundle cells, and improvement of aggregate structure

CN104262008AActive Publication Date: 2015-01-07JIANGMEN ZHIHU IND CO LTD

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, the specific preparation steps are as follows:

[0020] (1) Kelp pretreatment: rinse the kelp with clean water, remove impurities, and drain; cut the kelp into 3-5cm lengths with a knife; use 0.1% Ca(OH) with a pH of 6.5-7.0 2 The solution soaked Enteromorpha 3 times, each time for 15 minutes, and carried out desalination and color protection treatment; dried at 85°C, finally ground into powder, and passed through a 40-mesh sieve;

[0021] (2) Ultrasonic enzymatic hydrolysis: add water 8 times the mass of kelp, adjust the pH to 6.8-7.2; add 3% glucoamylase and 2% non-starch polysaccharase according to the mass ratio of kelp, ultrasonically assist enzymolysis at 40-60°C for 2 hours, and ultrasonically Power 400w, frequency 25kHz;

[0022] (3) Fermentation broth preparation: add 3.0% glucose, 2.0% yeast extract, 0.3% potassium dihydrogen phosphate, and 0.02% MgSO to the enzymolysis solutio...

Embodiment 2

[0027] A process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, the specific preparation steps are as follows:

[0028] (1) Enteromorpha pretreatment: rinse Enteromorpha with water, remove impurities, drain; cut Enteromorpha into 3-5cm lengths with a knife; use 0.1% Ca(OH) with pH6.5-7.0 2 The solution soaked Enteromorpha 3 times, each time for 15 minutes, and carried out desalination and color protection treatment; dried at 85°C, finally ground into powder, and passed through a 40-mesh sieve;

[0029] (2) Ultrasonic enzymolysis: add water 9 times the mass of Enteromorpha, adjust the pH to 6.8-7.2; add 2% glucoamylase and 1% non-starch polysaccharase according to the mass ratio of Enteromorpha, and ultrasonically assist enzymolysis at 40-60°C for 4 hours , ultrasonic power 400w, frequency 25kHz;

[0030] (3) Fermentation broth preparation: Add enteromorpha mass ratio of 5.0% glucose, 3.0% yeast extract, 0.5% potassium dihydrogen phosphate, 0.02% MgSO...

Embodiment 3

[0035] A process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, the specific preparation steps are as follows:

[0036] (1) Sargassum pretreatment: wash Sargassum with clean water, remove impurities, and drain; cut Sargassum into 3-5cm lengths with a knife; use 0.1% Ca(OH) with pH6.5-7.0 2 The solution soaked Enteromorpha 3 times, each time for 15 minutes, and carried out desalination and color protection treatment; dried at 85°C, finally ground into powder, and passed through a 40-mesh sieve;

[0037] (2) Ultrasonic enzymolysis: add water 10 times the mass of Sargassum, adjust the pH to 6.8-7.2; add 2.5% glucoamylase and 1.5% non-starch polysaccharase according to the mass ratio of Sargassum, and ultrasonically assist enzymolysis at 40-60°C for 3 hours , ultrasonic power 400w, frequency 25kHz;

[0038] (3) Fermentation broth preparation: Add 4.0% glucose, 2.5% yeast extract, 0.4% potassium dihydrogen phosphate, and 0.02% MgSO to the enzymolysis solu...

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Abstract

The invention discloses a process for preparing a liquid seaweed fertilizer through enzymolysis and fermentation. The process disclosed by the invention can be used for generating a large quantity of soil beneficial bacteria and multiple special-effect metabolites by degrading macromolecular substances which are contained in seaweeds and difficult to utilize into micromolecular polysaccharide, monosaccharide and a nitrogen-containing compound in compound enzymolysis and multi-strain co-fermentation modes, thereby enhancing the biological activity of a seaweed fertilizer, preventing the destruction of strong base, high temperature and high pressure in the production process of the seaweed fertilizer and completely retaining abundant nutrient substances contained in the seaweeds. According to the process, the content of a product, namely alginic acid, is 40-60 g / L and is increased by more than 30% compared with that prepared from an enzymolysis method, an alkaline hydrolysis method, an acid hydrolysis method and a simple microbe fermentation method, and the effective viable count is more than or equal to 2 billion / mL; the product integrates natural nutrient components, antibiotic substances and plant hormones, combines the efficacy of three fertilizers, namely an organic fertilizer, a microbe fertilizer and a trace element fertilizer and has a wide application prospect.

Description

technical field [0001] The invention relates to a process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, which belongs to the field of fertilizer production. Background technique [0002] Seaweed is a rich source of marine organisms, widely used in food, industrial raw materials, and herbal medicines. Seaweed has been used in agriculture and livestock production for centuries. Seaweed fertilizer is a new type of pure natural, non-toxic and side-effect fertilizer. It contains rich non-N organic matter and a certain amount of amino acids, proteins and micronutrients, and also contains seaweed polysaccharides, alginic acid, and alginic acid unique to marine organisms. Unsaturated fatty acids and mineral elements such as zinc, bromine and iodine, which are rare in terrestrial plants, and rich in vitamin C and B 1 , B 2 , B 12 , D, E, K and other nutrients have the functions of increasing crop yield, improving quality and enhancing crop resistance to...

Claims

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Application Information

Patent Timeline
07 Jan 2015
Publication
CN104262008A
IPC
C05G3/00; C05G3/04; C05G3/80
CPC
C05D9/02; C05G3/00; C05G3/80; C05F11/00; C05F11/08
Inventors
董书阁; 侯文杰