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Process for preparing leftover fertilizer processed through globefish by adopting enzymolysis and fermentation method

A technology of leftover material and fermentation method, which is applied in the field of preparing puffer fish waste material fertilizer by enzymatic hydrolysis and fermentation method, can solve the problems of improper utilization, pollution, loss and the like, and achieve the effect of realizing full utilization of resources.

Inactive Publication Date: 2016-03-02
QINGDAO WEIYUANDA TECH SERVICE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The export processing of puffer fish produces a large amount of leftovers such as fish skin, resulting in a great waste of resources and environmental pollution
Improper use, due to the presence of toxins in the leftovers, can easily cause unexpected losses

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A process for preparing fugu processing leftovers and fertilizers by enzymolysis and fermentation, the specific preparation steps are as follows:

[0017] (1) Pretreatment of leftovers: dry puffer fish leftovers, grind them into powder, and pass through a 40-mesh sieve;

[0018] (2) Ultrasonic enzymolysis: add water 8 times the mass of the leftovers, adjust the pH to 6.8-7.2; add 3% amylase and 2% non-starch polysaccharase according to the mass ratio of the leftovers, and ultrasonically assist enzymolysis at 40-60°C for 2 hours , ultrasonic power 400w, frequency 25kHz;

[0019] (3) Fermentation broth preparation: Add 3.0% glucose, 2.0% yeast extract, 0.3% potassium dihydrogen phosphate, 0.02% MgSO to the enzymolysis solution 4 .7H 2 O, 0.01%CaCl 2 , adjust the pH to 7.0-7.2;

[0020] (4) Aerobic fermentation: use a liquid fermentation tank with a liquid volume of 60-70% (volume ratio), and inoculate Bacillus subtilis, actinomycetes, and Aspergillus niger at 2%, 3%, ...

Embodiment 2

[0024] A process for preparing fugu processing leftovers and fertilizers by enzymolysis and fermentation, the specific preparation steps are as follows:

[0025] (1) Pretreatment of leftovers: dry puffer fish leftovers, grind them into powder, and pass through a 40-mesh sieve;

[0026] (2) Ultrasonic enzymolysis: add water 9 times the mass of the leftovers, adjust the pH to 6.8-7.2; add 2% amylase and 1% non-starch polysaccharase according to the mass ratio of the leftovers, and ultrasonically assist enzymolysis at 40-60°C for 4 hours , ultrasonic power 400w, frequency 25kHz;

[0027] (3) Fermentation broth preparation: Add 5.0% glucose, 3.0% yeast extract, 0.5% potassium dihydrogen phosphate, 0.02% MgSO to the enzymolysis solution 4 .7H 2 O, 0.01%CaCl 2 , adjust the pH to 7.0-7.2;

[0028] (4) Aerobic fermentation: use a liquid fermentation tank with a liquid volume of 60-70% (volume ratio), and inoculate Bacillus subtilis, actinomycetes, and Aspergillus niger with an ino...

Embodiment 3

[0032] A process for preparing fugu processing leftovers and fertilizers by enzymolysis and fermentation, the specific preparation steps are as follows:

[0033] (1) Pretreatment of leftovers: dry puffer fish leftovers, grind them into powder, and pass through a 40-mesh sieve;

[0034] (2) Ultrasonic enzymolysis: add water 10 times the mass of the leftovers, adjust the pH to 6.8-7.2; add 2.5% amylase and 1.5% non-starch polysaccharase according to the mass ratio of the leftovers, and ultrasonically assist enzymolysis at 40-60°C for 3 hours , ultrasonic power 400w, frequency 25kHz;

[0035] (3) Fermentation broth preparation: Add 4.0% glucose, 2.5% yeast extract, 0.4% potassium dihydrogen phosphate, 0.02% MgSO to the enzymolysis solution 4 .7H 2 O, 0.01%CaCl 2 , adjust the pH to 7.0-7.2;

[0036] (4) Aerobic fermentation: use a liquid fermentation tank with a liquid volume of 60-70% (volume ratio), and inoculate Bacillus subtilis, actinomycetes, and Aspergillus niger at 2.5...

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Abstract

The invention discloses a process for preparing leftover fertilizer processed through globefish by adopting an enzymolysis and fermentation method. A composite enzymatic hydrolysis and multi-strain fermenting mode is adopted for degrading macromolecule substances difficult to utilize in leftovers processed through the globefish into micromolecule polysaccharide, polypeptide and nitrogen-containing compounds, a large number of soil beneficial bacteria and various special-effect metabolites are generated, the biological activity of the leftover fertilizer processed through the globefish is improved, and pesticide and the fertilizer are combined into one, which is beneficial for pushing the development of the ecological agriculture and increasing the resource comprehensive utilization rate and the economic benefits of the globefish processing industry. The number of the effective active bacteria in the fertilizer is larger than or equal to 20 billion / mL. The fertilizer integrates natural nutrient ingredients, antibiotic substances and zoohormone, and has a composite effect of organic fertilizer, microbial fertilizer and microelement fertilizer; the fertilizer contains a certain amount of tetrodotoxin, and can effectively kill pests in soil, the pesticide and fertilizer combination is achieved, the duration time for the pest killing effect is long, and the application prospect is wide.

Description

technical field [0001] The invention relates to a process for preparing fugu processing leftover fertilizer by enzymolysis and fermentation, and belongs to the field of fertilizer manufacturing. Background technique [0002] Puffer fish( Fugurubripes ) contains the highly toxic substance tetrodotoxin, which has extremely high economic and medical value. People's research on puffer fish toxin is more in-depth and meticulous, but it ignores the value of other aspects of puffer fish. With the development of non-toxic puffer fish breeding and processing detoxification technology, the output of non-toxic puffer fish in my country has increased year by year, with an annual export rate of more than 90%, and it has become the main source of foreign puffer fish supply. The export processing of puffer fish produces a large amount of leftovers such as fish skin, which causes great waste of resources and environmental pollution. Improper use, because of the presence of toxins in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C12P1/02A01N63/00A01N63/02A01N63/04A01P21/00
CPCA01N63/00A01N63/10A01N63/30C12P1/02C12P1/04
Inventor 耿彩红韩昊
Owner QINGDAO WEIYUANDA TECH SERVICE CO LTD
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