A process for preparing seaweed liquid fertilizer by enzymolysis and fermentation

A liquid fertilizer and seaweed technology, which is applied in the field of seaweed liquid fertilizer preparation by enzymatic fermentation, can solve the problems of refractory macromolecular substances of seaweed, affecting the effect of seaweed fertilizer, and soil without any beneficial effect, so as to promote plant cell division, Effect of increasing vascular bundle cells and improving internal voids

Active Publication Date: 2017-03-22
JIANGMEN ZHIHU IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many defects in the preparation of seaweed fertilizers by biological fermentation methods. One is that the fermentation microorganisms are single, and the enzymes produced are single, which makes it difficult to truly degrade the macromolecules of seaweeds, which affects the effect of seaweed fertilizers. The second is the biological fermentation method. In the preparation of seaweed fertilizer, the use of microbial fermentation strains has no beneficial effect on the soil, which simply increases the production cost
Therefore, at present, most enterprises that use biological fermentation to produce seaweed fertilizers use the compound method of adding acid and alkali at the same time, which has the disadvantages of destroying and losing nutrients such as active factors in seaweed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, the specific preparation steps are as follows:

[0020] (1) Kelp pretreatment: rinse the kelp with clean water, remove impurities, and drain; cut the kelp into 3-5cm lengths with a knife; use 0.1% Ca(OH) 2 Soak the kelp in the solution for 3 times, each time for 15 minutes, and perform desalination and color protection treatment; dry at 85°C, finally grind it, and pass through a 40-mesh sieve;

[0021] (2) Ultrasonic enzymatic hydrolysis: add water 8 times the mass of kelp, adjust the pH to 6.8-7.2; add glucoamylase and non-starch polysaccharide enzyme, the addition amount is 3% and 2% of the mass of kelp, ultrasonic at 40-60°C Auxiliary enzymatic hydrolysis for 2 hours, ultrasonic power 400w, frequency 25kHz;

[0022] (3) Fermentation broth preparation: add glucose, yeast extract, potassium dihydrogen phosphate, MgSO to the enzymolysis solution 4 ·7H 2 O, CaCl 2 , the addition amount...

Embodiment 2

[0027] A process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, the specific preparation steps are as follows:

[0028] (1) Enteromorpha pretreatment: rinse Enteromorpha with water, remove impurities, drain; cut Enteromorpha into 3-5cm lengths with a knife; use 0.1% Ca(OH) 2 The solution soaked Enteromorpha 3 times, each time for 15 minutes, and carried out desalination and color protection treatment; dried at 85°C, finally ground into powder, and passed through a 40-mesh sieve;

[0029] (2) Ultrasonic enzymatic hydrolysis: add water 9 times the mass of Enteromorpha, adjust the pH to 6.8-7.2; add glucoamylase and non-starch polysaccharide enzyme, the addition amount is 2% and 1% of the mass of Enteromorpha, at 40-60°C Ultrasonic assisted enzymolysis for 4 hours, ultrasonic power 400w, frequency 25kHz;

[0030] (3) Fermentation broth preparation: add glucose, yeast extract, potassium dihydrogen phosphate, MgSO to the enzymolysis solution 4 ·7H 2 O, ...

Embodiment 3

[0035] A process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, the specific preparation steps are as follows:

[0036] (1) Sargassum pretreatment: rinse Sargassum with clean water, remove impurities, and drain; cut Sargassum into 3-5cm lengths with a knife; use 0.1% Ca(OH) 2 The solution soaked the Sargassum 3 times, each time for 15 minutes, and carried out desalination and color protection treatment; dried at 85°C, finally pulverized, and passed through a 40-mesh sieve;

[0037] (2) Ultrasonic enzymatic hydrolysis: add water 10 times the mass of Sargassum, adjust the pH to 6.8-7.2; add glucoamylase and non-starch polysaccharase, the addition amount is 2.5% and 1.5% of the mass of Sargasso, at 40-60°C Ultrasonic-assisted enzymatic hydrolysis for 3 hours, ultrasonic power 400w, frequency 25kHz;

[0038] (3) Fermentation broth preparation: add glucose, yeast extract, potassium dihydrogen phosphate, MgSO to the enzymolysis solution 4 ·7H 2 O, CaCl ...

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PUM

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Abstract

The invention discloses a process for preparing a liquid seaweed fertilizer through enzymolysis and fermentation. The process disclosed by the invention can be used for generating a large quantity of soil beneficial bacteria and multiple special-effect metabolites by degrading macromolecular substances which are contained in seaweeds and difficult to utilize into micromolecular polysaccharide, monosaccharide and a nitrogen-containing compound in compound enzymolysis and multi-strain co-fermentation modes, thereby enhancing the biological activity of a seaweed fertilizer, preventing the destruction of strong base, high temperature and high pressure in the production process of the seaweed fertilizer and completely retaining abundant nutrient substances contained in the seaweeds. According to the process, the content of a product, namely alginic acid, is 40-60 g / L and is increased by more than 30% compared with that prepared from an enzymolysis method, an alkaline hydrolysis method, an acid hydrolysis method and a simple microbe fermentation method, and the effective viable count is more than or equal to 2 billion / mL; the product integrates natural nutrient components, antibiotic substances and plant hormones, combines the efficacy of three fertilizers, namely an organic fertilizer, a microbe fertilizer and a trace element fertilizer and has a wide application prospect.

Description

technical field [0001] The invention relates to a process for preparing seaweed liquid fertilizer by enzymolysis and fermentation, which belongs to the field of fertilizer production. Background technique [0002] Seaweed is a rich source of marine organisms, widely used in food, industrial raw materials, and herbal medicines. Seaweed has been used in agriculture and livestock production for centuries. Seaweed fertilizer is a new type of pure natural, non-toxic and side-effect fertilizer. It contains rich non-N organic matter and a certain amount of amino acids, proteins and micronutrients, and also contains seaweed polysaccharides, alginic acid, and alginic acid unique to marine organisms. Unsaturated fatty acids and mineral elements such as zinc, bromine and iodine, which are rare in terrestrial plants, and rich in vitamin C and B 1 , B 2 , B 12 , D, E, K and other nutrients have the functions of increasing crop yield, improving quality and enhancing crop resistance to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00C05G3/04C05G3/80
CPCC05D9/02C05G3/00C05G3/80C05F11/00C05F11/08
Inventor 董书阁侯文杰侯文燕董静静段智岗代书强
Owner JIANGMEN ZHIHU IND CO LTD
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