Method for producing protein feed through mixed strain solid-state fermentation of ginkgo leaf residue

A technology of ginkgo biloba residue and solid-state fermentation, applied in animal feed, animal feed, application, etc., can solve the problems of low nutritional value, low protein content of fermented feed, poor digestion and absorption, etc., and achieve the effect of high nutritional value

Active Publication Date: 2014-01-01
日照恒邦牧业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These patents all use a single strain of bacteria for fermentation, and the prepared biological feed has problems such as low protein content, single nutrient composition, poor digestion and absorption, and poor palatability.
In the preparation of biological feed from ginkgo leaf residue, only patent 201010295193.x reports that the ginkgo drug residue and straw ar...

Method used

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  • Method for producing protein feed through mixed strain solid-state fermentation of ginkgo leaf residue
  • Method for producing protein feed through mixed strain solid-state fermentation of ginkgo leaf residue
  • Method for producing protein feed through mixed strain solid-state fermentation of ginkgo leaf residue

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Experimental program
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Effect test

Embodiment 1

[0049] Strain culture

[0050] a. Expansion culture of Candida tropicalis: activate Candida tropicalis and inoculate it into a large test tube containing 5 mL of wort medium, culture it at 28°C for 24 hours, and then inoculate it with 5% inoculum in a large test tube containing 500 mL of wort medium. In the Erlenmeyer flask of the wort medium, culture it on a constant temperature shaker (180r / min) at 28°C for 36h to obtain a liquid strain of Candida tropicalis.

[0051] b. Amplification culture of Aspergillus oryzae: activate Aspergillus oryzae and inoculate it into a large test tube containing 5mL Czapek's liquid medium, culture at 28°C for 24h, and then inoculate 5% of the inoculum into a triangle containing 500mL Czapek's liquid medium In the bottle, culture it on a constant temperature shaker (180r / min) at 28°C for 36h to obtain liquid strains of Aspergillus oryzae.

[0052] c. Expansion culture of Bacillus subtilis: activate Bacillus subtilis and inoculate it into a larg...

Embodiment 2

[0061] Preparation of solid-state fermentation medium

[0062] After drying and crushing the ginkgo leaf residue, add water 2 to 4 times the weight of the ginkgo leaf residue, then add a nitrogen source with 5 to 12% of the weight of the ginkgo leaf residue, mix well, and steam sterilize at 120 to 125°C for 20 ~30min, after cooling, the solid-state fermentation medium of ginkgo biloba residue is obtained.

[0063] The ginkgo leaf residue is obtained by air-drying the leaf residue after extracting ginkgo flavonoids from young ginkgo leaves of 3 to 6 years old.

[0064] The nitrogen source is urea or ammonium sulfate.

[0065] Water is one of the basic substances necessary for microbial fermentation. Each microorganism has the most suitable water content for its growth. This is because water is an important part of microbial cells and a medium for microbial metabolic activities. It also participates in part of the biochemical process. reaction.

[0066]The present invention ...

Embodiment 3

[0074] Preparation of Protein Feed by Solid State Fermentation

[0075] Candida tropicalis liquid strains, Aspergillus oryzae liquid strains, Bacillus subtilis liquid strains and Lactobacillus plantarum liquid strains were respectively inserted into the solid-state fermentation medium of ginkgo leaf residue according to a certain strain ratio, and the four liquid strains The total amount of inoculation is 8-12% of the solid-state fermentation medium. After the inoculation is completed, mix well, then ferment statically at a temperature of 28-30°C and at a natural pH value for 72-120 hours, and finally ferment the product at 50°C. Dried to constant weight to obtain ginkgo biloba residue protein feed.

[0076] The ratio of the introduced strains of the Candida tropicalis liquid strain, the Aspergillus oryzae liquid strain, the Bacillus subtilis liquid strain and the Lactobacillus plantarum liquid strain is 1-2:2:1-2:1.

[0077] The present invention investigates the ratio of co...

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Abstract

The invention relates to a method for producing a protein feed by using gingko leaf residue as a raw material for mixed strain solid-state fermentation. Activation and multiplication culture are respectively performed on candida tropicalis, aspergillus oryzae, bacillus subtilis and lactobacillus plantarum, so as to obtain four liquid strains; after the gingko leaf residue is dried and smashed, a certain amount of water and a nitrogen source are added, and after mixing and sterilizing, the gingko leaf residue is taken as a solid-state fermentation culture medium; the four liquid strains are inoculated to the solid-state fermentation culture medium according to certain proportions, after sufficient mixing, fermentation for 72 to 120 hours can be performed at a nature pH value under the temperature of 28 to 30 DEG C, and after drying, a gingko leaf residue protein feed can be obtained. In the protein feed, the coarse protein content of the protein feed is high and can reach 46 percent, the nutritional ingredients are complete, the palatability is excellent, the production performance and the feed conversion rate of livestock and poultry can be effectively improved, the preparation method is simple in process, the cost is low, and the mass production can be achieved. The invention further provides a way for using the gingko leaf residue which is a waste material in forestry processing.

Description

technical field [0001] The invention belongs to the technical field of agricultural and forestry waste processing and utilization, and in particular relates to a method for producing protein feed by solid-state fermentation of mixed bacteria by using ginkgo biloba residue as raw material. Background technique [0002] With the rapid development of my country's animal husbandry and feed industry, my country's demand for feed raw materials has increased sharply, making my country's feed resources, especially protein resources, more scarce. According to statistics, by 2012, the national supply of protein feed was 22 million tons , the demand is 66 million tons, which shows that the supply of protein feed in my country has been seriously insufficient. On the one hand, there is a lack of protein feed resources in my country. On the other hand, due to the long-term use of antibiotics for feeding, most of the existing feeds have low nutritional value and are not easily digested and ...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14
Inventor 王成章周昊
Owner 日照恒邦牧业科技股份有限公司
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