This invention belongs to the fields of
bioinformatics and
molecular biology, specifically relating to a method for identifying
gene duplication and functional differentiation in
Rosaceae genera. By analyzing MAPK family proteins in rose and
Arabidopsis thaliana, the invention identifies HT1, ANP3, Raf42, and Raf24 proteins that are duplicated and functionally differentiated in rose. Further application of
bioinformatics and
molecular biology techniques to
Rosaceae plants reveals that only ANP3 exhibits duplication and functional differentiation in all
Rosaceae plants. This invention provides a molecular-level reference for exploring the function of the MAPK family and the functional differentiation among ANP3-like genes in Rosaceae, laying the foundation for future research and identification of other duplicated and functionally differentiated genes in Rosaceae.