9 results about "Rosaceae (plant)" patented technology

This invention relates to compositions and methods for modifying TFL genes in Rosaceae plants to remove or reduce the dependency on environmental triggers for flowering, optionally producing plants having improved characteristics for breeding and production, optionally, including a reduced time to initiate flowering, a longer duration of flowering, and / or improved yield characteristics. The invention further relates to Rosaceae plants produced using the methods and compositions of the invention.

The invention belongs to but not limited to the technical field of microbial application, and discloses a plant immune resistance inducer as well as a preparation method and application thereof. Comprising an Aava fermentation extract, a surfactant, a defoaming agent, an anti-freezing agent, a thickening agent and a solvent, the Aava fermentation extract is a concentrate of an endophytic alternaria Aava fermentation filtrate, the surfactant is sodium dodecyl sulfate, the defoaming agent is tributyl phosphate, the antifreezing agent and the thickening agent are glycerol, and the solvent is water. The Aava fermentation extract disclosed by the invention is realized by stimulating autoimmunity of kiwi fruit plants, has no any sterilization effect on canker pathogens, and is not easy to generate drug resistance; and the compound has a certain broad spectrum, and has an induced resistance effect on gramineous plant wheat, rosaceae plant apple and solanaceae plant tobacco. When the Aava fermentation extract is used as a plant immune resistance inducer, the defense capability of plants can be improved, and the canker resistance of the plants can be remarkably improved.

The present disclosure relates to cultivated Rosaceae plants, such as strawberries, blackberries, and raspberries, having an early flowering trait resulting from genetically engineered Terminal Flowering alleles (TFL1) or homologs thereof that reduce or knockout TFL1 protein function. The disclosure further teaches methods of producing the same.

The present invention relates to compositions and methods for modifying WUSCHEL (WUS) related homologous box (WOX) functional genes in plants of the Rosaceae family, optionally to improve the configuration of thorns and / or thorns, to reduce the number and / or size of thorns and skin thorns, or to eliminate thorns and / or skin thorns. The present invention also relates to plants of the Rosaceae family having thorns and / or skin thorns of improved configuration, a reduced number and / or size, or no thorns and / or skin thorns produced using the methods and compositions of the present invention. The invention further relates to a method for identifying and / or cultivating a plant having thorns and / or barks of improved configuration, a reduced number and / or size, or no thorns and / or barks.

The invention discloses a key gene RcERF110 for regulating stress resistance of Chinese rose and application of the key gene RcERF110, the nucleotide sequence of the key gene RcERF110 is as shown in SEQ ID No.1, the amino acid sequence of the key gene RcERF110 is as shown in SEQ ID No.2, and the expression mode of the gene RcERF110 is obviously induced by drought stress and high temperature stress. A genetic transformation experiment shows that the drought tolerance and the high temperature resistance of a transgenic plant can be remarkably enhanced by overexpressing the gene in a model plant tobacco. Further, a gene silencing experiment in Chinese rose proves that the dehydration tolerance of petal tissues can be remarkably reduced and the heat stress resistance of plants can be weakened by inhibiting the expression of the gene RcERF110. The invention not only provides a new theoretical basis for analyzing a rosaceae plant abiotic stress response molecular mechanism, but also lays an important foundation for creating a drought-resistant and heat-resistant Chinese rose new germplasm through a molecular breeding technology, and has an important application value for horticultural plant stress resistance genetic improvement.

This invention belongs to the fields of bioinformatics and molecular biology, specifically relating to a method for identifying gene duplication and functional differentiation in Rosaceae genera. By analyzing MAPK family proteins in rose and Arabidopsis thaliana, the invention identifies HT1, ANP3, Raf42, and Raf24 proteins that are duplicated and functionally differentiated in rose. Further application of bioinformatics and molecular biology techniques to Rosaceae plants reveals that only ANP3 exhibits duplication and functional differentiation in all Rosaceae plants. This invention provides a molecular-level reference for exploring the function of the MAPK family and the functional differentiation among ANP3-like genes in Rosaceae, laying the foundation for future research and identification of other duplicated and functionally differentiated genes in Rosaceae.

The present disclosure relates to cultivated Rosaceae plants, such as strawberries, blackberries, and raspberries, having an early flowering trait resulting from genetically engineered Terminal Flowering alleles (TFL1) or homologs thereof that reduce or knockout TFL1 protein function. The disclosure further teaches methods of producing the same.

The invention discloses a rosaceae plant embryonic stem cell acquisition method based on an ultrasonic synergistic hormone regulation system, and belongs to the technical field of plant stem cells. According to the method, rosaceae young fruits (such as apples and strawberries) with the development period of 30-50 days are used as explants, the pollution rate is reduced to be smaller than or equal to 5% through gradient disinfection (detergent-ethanol-sodium hypochlorite), embryo tissue is broken through 20kHz pulse ultrasonic waves (working for 1 second / intermittent for 5 seconds), and the tissue is subjected to tissue culture. Efficient separation and amplification of the stem cells are realized by combining a specific hormone proportioning culture medium; an induction culture medium (MS + 0.1-0.5 mg / L of GA3 + 0.1-0.5 mg / L of KT + 0.2 mg / L of cephalosporin) activates the activity of the stem cells to 88.5 + / -3.2%; the proliferation culture medium (MS + 0.5-1.0 mg / L 6-BA + 0.1-0.2 mg / L NAA + 0.1-0.5 mg / L KT + 0.2 mg / L cephalosporin) enables the cell density to reach 1.85 * 10 cells / mL, and the cell density is increased by 230% compared with that of a traditional method. The method breaks through the limitation that a traditional enzymolysis method is low in efficiency and high in damage, the high-purity embryonic stem cells can be obtained within 7 days, and an efficient cell platform is provided for development of medicinal components of the rosaceae and variety improvement.

The invention discloses a bacillus TRMB57781. The preservation number of the bacillus TRMB57781 is CGMCC (China General Microbiological Culture Collection Center) No.36226; the paenibacillus TRMB57781 is used for antagonizing plant pathogenic bacteria or is used for preparing a cyclic lipopeptide antibiotic Fusaricidin B. The paenibacillus TRMB57781 has the advantages that the paenibacillus TRMB57781 can be The invention further discloses a preparation of the paenibacillus TRMB57781, and the preparation of the paenibacillus TRMB57781 is prepared from the paenibacillus TRMB57781. The bacillus TRMB57781 preparation can be used for preventing and treating pear fire blight of rosaceae plants, not only can achieve the prevention and treatment effect equivalent to that of chemical prevention and treatment, but also can avoid ecological and safety problems caused by the chemical prevention and treatment.