Streptomyces lydicus and application thereof to prevention and treatment of fire blight

A technology for Streptomyces lydidea and fire blight, applied in the field of Streptomyces lydidea and its application in the biological control of fire blight, can solve the problems of lack of research and reports, late research on Streptomyces lydidea, etc., and achieve good control effect, markedly antagonistic effect, markedly effective effect

Active Publication Date: 2019-06-04
NANJING UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, the research on Streptomyces lydida is relatively late, and there is no research and report on its prevention and control of bacterial diseases such as fire blight

Method used

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  • Streptomyces lydicus and application thereof to prevention and treatment of fire blight
  • Streptomyces lydicus and application thereof to prevention and treatment of fire blight
  • Streptomyces lydicus and application thereof to prevention and treatment of fire blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: Isolation and screening of Streptomyces lydidia M01

[0038] 1. Sampling the root soil of wild bean pears in Laoshan Forest Park, Nanjing, using the plate dilution coating method to isolate actinomycetes, grinding the air-dried soil samples with a mortar, weighing 10g and adding them to a triangular flask filled with 90mL sterile water , 200rpm / min on a constant temperature shaking shaker at 28°C - After shaking for 30 minutes, let stand for 10 minutes.

[0039] 2. Take 1mL of the above homogenate for 10-fold gradient dilution, and finally prepare 10 -3 、10 -4 、10 -5 100ul of the suspensions with different concentrations were drawn and added to the modified Gao's No. 1 medium plate, spread evenly and then cultured at 28°C. After 5 to 7 days, different single colonies were picked and streaked for purification.

[0040] 3. Using the Erwinia amylovora indicator bacteria, use the plate confrontation method to screen: absorb the Erwinia amylovora culture so...

Embodiment 2

[0041] Embodiment 2: Species identification and physiological and biochemical index determination of Streptomyces lydida M01

[0042] 1, the bacterial strain M01 that embodiment 1 is obtained obtains its pure culture after streaking pure culture on ISP-2 substratum (attachment figure 1 ), observed under a microscope after Gram staining, which belonged to Gram-positive filamentous bacteria with well-developed mycelium, branched mycelium, and no septum. The hyphae in the base are not broken, and the aerial hyphae are well developed, forming long (sometimes short) spore filaments. The spores cannot move, and there are often decorations such as warts, thorns or hairs on the outer sheath (attached figure 2 ).

[0043] 2. Determine the morphological and physiological and biochemical characteristics of the strain, observe the morphology of the strain M01 on different solid media, and determine through a series of experiments such as gelatin liquefaction, starch hydrolysis, V-P react...

Embodiment 3

[0048] Embodiment 3: Streptomyces lydidi M01 antibacterial activity assay in vitro

[0049] 1. Using Staphylococcus aureus, Escherichia coli, Pseudomonas putida, Bacillus subtilis, Pantoea pineapple and Erwinia amylovora as indicator bacteria, the in vitro antibacterial activity of Streptomyces lydidi M01 on bacteria was determined by plate confrontation method . The specific embodiment refers to embodiment 1.

[0050] 2. See Table 2 for the average diameter of the inhibition zone of Streptomyces lydidi M01 fermentation broth to different indicator bacteria; Figure 5 It is a diagram showing the inhibitory effect of the Streptomyces lydida M01 fermentation liquid of the present invention on the fire blight pathogen Erwinia amylovora.

[0051] Table 2 Inhibition diameter of Streptomyces lydidi M01 to different indicator bacteria

[0052]

[0053] "+": positive results; "-": negative results.

[0054] The experimental results show that Streptomyces lydidis M01 has a broad...

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Abstract

The invention discloses streptomyces lydicus. The classification name of the streptomyces lydicus is streptomyces lydicus (streptomyces lydicus), the bacterial strain number of the streptomyces lydicus is M01, the streptomyces lydicus is preserved in the China General Microbiological Culture Collection Center, the preservation date is 29 November 2018, and the preservation number is CGMCC No.16840. The invention also discloses an application of the streptomyces lydicus to prevention and treatment of fire blight of plants. The streptomyces lydicus disclosed by the invention has remarkable antagonizing effects on erwinia amylovora (erwinia amylovora) as a pathogenic bacterium of the fire blight, and has favorable prevention and treatment effects on the fire blight of rosaceae plants especially industrial crops of pear trees, apple trees, cherry-apple trees and the like. Compared with chemical agricultural chemicals for prevention and treatment of plant diseases, the streptomyces lydicusdisclosed by the invention is more notable on effects, is pollution-free to environment, facilitates production of green and nuisance free products, is a biological prevention bacterial strain havinggreat application prospects, and can be used for preparing functional biological prevention microbial inoculum for preventing and treating the fire blight.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, in particular to a strain of Streptomyces lydidii and its application in biological control of fire blight. Background technique [0002] Erwinia amylovora is a destructive phytopathogenic bacterium that can infect pears, apples, and more than 220 species of plants belonging to more than 40 genera of Rosaceae, causing fire blight and causing serious economic losses. The disease was first observed in New York State, USA in 1780, and has spread to 43 countries including North America, Europe, the Middle East and New Zealand. Pear fire blight affects the flowers, buds, leaves, branches and trunks of trees, giving them a charred appearance. The pathogen first infects the flower stigma or wound tissue of the plant, and then multiplies rapidly, causing ulcers on young shoots, branches and trunks, leading to the death of trees. Infected flower organs are wilted, dark brown, and spr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/465
Inventor 徐虹许宗奇马俊杰王明轩詹伊婧冯小海李莎
Owner NANJING UNIV OF TECH
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