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Preparation method of 2alpha, 3beta-dihydroxyl oleanane-13(18)-ene-28-acid and application of 2alpha, 3beta-dihydroxyl oleanane-13(18)-ene-28-acid in preparing antibacterial agent

A technology of dihydroxyoleanane and antibacterial agent, applied in the field of 2α, the preparation of antibacterial agent, can solve the problem of no antibacterial activity, etc., and achieve the effect of broad antibacterial activity spectrum, wide application potential and easy storage

Inactive Publication Date: 2013-11-27
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are no reports on its antibacterial activity

Method used

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  • Preparation method of 2alpha, 3beta-dihydroxyl oleanane-13(18)-ene-28-acid and application of 2alpha, 3beta-dihydroxyl oleanane-13(18)-ene-28-acid in preparing antibacterial agent
  • Preparation method of 2alpha, 3beta-dihydroxyl oleanane-13(18)-ene-28-acid and application of 2alpha, 3beta-dihydroxyl oleanane-13(18)-ene-28-acid in preparing antibacterial agent
  • Preparation method of 2alpha, 3beta-dihydroxyl oleanane-13(18)-ene-28-acid and application of 2alpha, 3beta-dihydroxyl oleanane-13(18)-ene-28-acid in preparing antibacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Preparation of 2α, 3β-dihydroxyoleanane-13(18)-ene-28-acid from the fruit of Rosa rosae

[0024] 1.1 Plant source and identification

[0025] The fruit sample of Rosa laevigata Michx., a plant material for extraction, was collected from Hunan Province in September 2010, and was identified by Xing Fuwu, a researcher at South China Botanical Garden, Chinese Academy of Sciences.

[0026] 1.2 Extraction and separation

[0027] The sample (dried fruit of Rosa japonica, weighing 2.5 kg) was crushed and extracted three times with 95% ethanol aqueous solution at room temperature, and the combined extracts were concentrated under reduced pressure to remove ethanol to obtain the crude extract of the total extract. Suspend the crude extract of the total extract in 500ml of water, then extract it with an equal volume of petroleum ether, and then extract it three times with ethyl acetate. The ethyl acetate extract is concentrated under reduced pressure to obtain the tota...

Embodiment 2

[0034] Example 2: Detection of antibacterial activity of compound 2α, 3β-dihydroxyoleanane-13(18)-ene-28-acid

[0035] 2.1 Types of bacteria used in experiments

[0036] Gram-positive bacteria: Staphyloccocus aureus, Bacillus thuringiensis;

[0037] Gram-negative bacteria: Escherichia coli, Salmonella enterica, Shigella dysenteriae.

[0038] 2.2 Experimental drugs

[0039] Positive control substance: kanamycin sulfate (kanamincy sulfate)

[0040] Negative control: MeOH

[0041] 2α, 3β-dihydroxyoleanane-13(18)-ene-28-acid is prepared by the above experimental example

[0042] 2.3 Experimental method:

[0043] 2α, 3β-Dihydroxyoleanane-13(18)-ene-28-oic acid and kanamycin sulfate were respectively prepared in methanol (MeOH) into 1 mg / ml solutions. The minimum inhibitory concentration (MIC) of five bacterial strains was determined using a 96-well cell culture plate by resazurin color reaction method.

[0044] Firstly, 100 μg / mL resazurin indicator was added to the 11th col...

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Abstract

The invention provides a preparation method of 2alpha, 3beta-dihydroxyl oleanane-13(18)-ene-28-acid and an application of 2alpha, 3beta-dihydroxyl oleanane-13(18)-ene-28-acid in preparing an antibacterial agent. A potent antibacterial agent (medicine) is extracted and separated from Rosaceae Rose fructus rosae laevigatae widely distributed in China. The acid is abundant in plant source and convenient to extract. The plant is not damaged but utilized for a long time when plant fruits are extracted. The acid provided by the invention is environmentally-friendly and has better economic benefit. The monomer compound product is stable and easy to store. The acid is wide in bacteriostatic activity spectrum and high in bacteriostatic activity, can be further developed as an effective and safe antibacterial agent of foods such as fruits and vegetables and novel anti-infection medicines, and has good market prospect.

Description

Technical field: [0001] The invention belongs to the field of antibacterial, antiseptic and medical technology of fruits and vegetables, and specifically relates to a preparation method of 2α, 3β-dihydroxyoleanane-13(18)-ene-28-acid and its application in the preparation of antibacterial agents. Background technique: [0002] Bacterial infection of food is an important way for human beings to infect diseases, and the problem of drug resistance of pathogenic bacteria is also posing a more and more significant threat to people's health. The development of effective and safe antibacterial agents and new anti-infective drugs for foods such as fruits and vegetables has always had a realistic and extensive demand. [0003] Rosa laevigata Michx. is a medicinal and edible plant of the genus Rosa in the family Rosaceae, also known as white jade belt, elm thorn, golden pot bottle, Xiashanhu, and three-leaf leaf. It is widely distributed in my country and has rich resources. Its extrac...

Claims

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Application Information

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IPC IPC(8): A01N37/36A01P1/00A61K31/56A61P31/04C07J63/00
CPCY02A50/30
Inventor 谭建文王晶徐巧林任慧罗应
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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