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Sorbus alnifolia tissue culture and rapid propagation method

A technique for rapid propagation by tissue culture of Aronia serrata, applied in the field of plant tissue culture, can solve the problems of resource exhaustion, low seed reproduction rate, insufficient seed collection resources, etc. effect of value

Inactive Publication Date: 2015-06-10
朱炳贵
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to insufficient seed collection resources, low seed reproduction rate, and long seedling breeding cycle, the resources of Sorbus chinensis are increasingly depleted, resulting in a situation in which Sorbus elm seedlings are in short supply in the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Seed disinfection and sowing: Select the Sorbus elm seeds of the year, and treat them at 2°C for 45 days. Select plump seeds and wash them under running water for 1.5 hours, soak them in 75% ethanol solution for 35 seconds in an ultra-clean workbench, rinse them with sterile water for 3 times, then disinfect them with 0.1% mercuric solution for 10 minutes, rinse them with sterile water for 3 times before using them. The moisture on the surface was blotted with sterile filter paper, the complete seed embryo was taken out, and the radicle was vertically inserted into the seed germination medium for cultivation. After inoculation, the seed germination rate reached 93.6% after being cultured for 30 days under the conditions of 13 hours of light per day, light intensity of 2500lx, culture temperature of 26°C, and relative air humidity of 75%. The germination medium is: MS+3.0mg / LGA 3 +1.5g / L AC+18g / L sucrose+4.5g / L agar, the pH is 5.7.

[0019] (2) Induction culture: C...

Embodiment 2

[0024] (1) Seed disinfection and sowing: Select the Sorbus elm seeds of that year, and treat them at a low temperature of 3°C for 50 days. Pick plump seeds and wash them under running water for 2 hours, soak them in 75% ethanol solution for 40 seconds in an ultra-clean workbench, rinse them with sterile water for 4 times, then disinfect them with 0.1% mercuric chloride solution for 15 minutes, rinse them with sterile water for 3 times, and then rinse them with sterile water. Bacteria filter paper blots the water on the surface, takes out the complete seed embryo, and inserts the radicle vertically into the seed germination medium for cultivation. After inoculation, the seed germination rate reached 95.3% after being cultivated for 30 days under the conditions of 14 hours of light per day, 2500lx of light intensity, 27°C of culture temperature and 75% relative air humidity. The germination medium is: MS+4.0mg / LGA 3 +2.0g / L AC+20g / L sucrose+4.5g / L agar, the pH is 5.7.

[0025]...

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PUM

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Abstract

The invention discloses a sorbus alnifolia tissue culture and rapid propagation method. Sorbus alnifolia belongs to the rosaceae sorbus deciduous trees, is a precious timber and a tree species in east China and north China, is a fine tree species for landscaping of northern cities and has a high medicinal value. A sorbus alnifolia seed is used as an explant, an aseptic seedling is obtained and is subjected to induction culture, multiplication culture, rooting culture, acclimatization, transplanting and the like to obtain the sorbus alnifolia in-vitro regeneration plant, the sorbus alnifolia tissue culture and rapid propagation technology system is established and the method has important practical significance on the promotion of the sorbus alnifolia scale development.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Sorbus elm. Background technique [0002] Sorbus elm ( Sorbus alnifolia ) is a deciduous tree belonging to Rosaceae Sorbus, mainly distributed in Northeast, North, Northwest, Central and East China. Sorbus elm has twinkling white flowers in spring, feathery leaves in summer, and golden leaves and red fruits in autumn. It is really a beautiful landscaping and mountain afforestation tree species. Its wood has beautiful color and fine structure. It can be used as utensils and furniture, and its bark can also be used as dye. It has become a precious timber tree species in our country. Its fruit and stem bark have high medicinal value, have the effects of promoting blood circulation and dispersing blood stasis, relieving cough and lowering qi, and the fruit can also be used to make wine and jam. ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 朱炳贵
Owner 朱炳贵
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