Use of transgenic terrestrial plants in eutrophic water restoration

Abstract

The invention belongs to the field of biological phosphorus accumulating technology, and relates to a method for removing soluble phosphorus from eutrophic water by using transgenic terrestrial plants. According to the present invention, the transgenic terrestrial plants comprise tobacco in Solanaceae and strawberry in Rosaceae, and the imported exogenous gene is polyphosphate kinase gene (ppk); after the transgenic plants are induced by water roots, a foam plate sealed with a nylon net is adopted to fix the transgenic plants, which float on the water surface; in the high concentration soluble phosphorus (50 mg / ml)-containing water, the maximum phosphorus removal rates of the transgenic tobacco and the transgenic strawberry are respectively 57.4% and 41.38%; in the low concentration soluble phosphorus (100 mug / ml)-containing water, the maximum phosphorus removal rates of the transgenic tobacco and the transgenic strawberry are respectively 98.8% and 94.3%. The method of the present invention is applicable for efficient biological removal of the phosphorus, biological treatment of the phosphorus-rich wastewater, biological restoration of the eutrophic water, and biological purification of the eutrophic water.

Description

technical field [0001] The invention belongs to the technical field of biological phosphorus accumulation, and relates to a method for removing soluble phosphorus in eutrophic water by using transgenic terrestrial plants, which is used for the efficient biological removal of phosphorus, and the treatment of phosphorus-rich biological sewage, and the biological treatment of eutrophic water. Restoration and bio-decontamination. Background technique [0002] my country is a country with very poor water resources. The total amount of fresh water resources is about 2.8 trillion cubic meters, and the per capita possession is about 2,300 cubic meters, which is only 1 / 4 of the world's average level. It ranks 110th in the world and is the world's per capita One of the most water-scarce countries. The increasingly serious water pollution and water use situation is even more severe. The overall pollution status of the four major river basins of the Yellow River, Yangtze River, Huaihe ...

AI Technical Summary

Problems solved by technology

However, activated sludge and EBPR are mainly suitable for the preliminary treatment of sewage in sewage treatment plants, and are difficult to be used for in-situ restoration of eutrophic natural water bodies caused by phosphorus. The reasons are as follows: (1) If activated sludge is directly put into natural water bodies, its The enriched phosphorus is still in the sludge, and it is easy to re-hydrolyze polyphosphorus into phosphate and enter the water body again; (2) EBPR technology requires aeration and anaerobic cycle, which cannot be realized in natural water bodies; (3) Phosphorus-accumulating bacteria themselves have limited ability to accumulate phosphorus. All phosphorus-accumulating bacteria that have been found so far have a phosphorus-accumulating enzyme that catalyzes the polymerization of phosphate into polyphosphate and a phospholytic enzyme that catalyzes the hydrolysis of polyphosphate into soluble phosphate. The promoter in the operon is a phosphorus-regulated promoter, and the phosphorus concentration required for induction of this promoter is much higher than the phosphorus concentration in the national V water standard, so the phosphorus concentration that induces eutrophication in natural water bodies is difficult to activate phosphorus-accumulating enzymes Gene expression; similarly, the tandem composition of phosphorus-accumulating enzyme and phospholytic enzyme makes the expression of phosphorus-accumulating enzyme and phospholytic enzyme synchronously expressed, which leads to a dynamic balance between phosphorus-accumulating and phosphorus-dissolving enzymes, and it is difficult to enrich phosphorus in natural water bodies

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