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Establishment method of S.Davidianavar. undulata. isolated culture regeneration system

A technology for in vitro culture and establishment method, which is applied in the establishment field of in vitro culture and regeneration system of Boye Hongguo, which can solve the problems of low efficiency, inability to adapt to large-scale and standardized production, and high cost, so as to improve reproduction efficiency and speed up reproduction and promotion effect

Inactive Publication Date: 2015-07-22
冯文杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the seedling propagation of Boyehongguo tree is mainly carried out by seed propagation, cutting propagation, etc., which has problems such as long cycle, low efficiency, and high cost, and cannot meet the needs of large-scale and standardized production. Therefore, it is necessary to build a separate In vivo culture regeneration system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Aseptic sowing of seeds: peel the seeds from the fruit and place them in a beaker, wash them with tap water, rinse them under tap water for 1 hour, and then rinse them with distilled water for 3 times. Place the rinsed seeds on an ultra-clean workbench, soak them in 75% ethanol for 5 seconds, and use 0.1% HgCl 2 Soak and disinfect for 5 minutes, and finally rinse with sterile water 4 times. The seed coats of the sterilized seeds are peeled off in a sterile petri dish to obtain mature seed embryos, which are then inoculated into a seed germination medium. After inoculation, cultivate under dark conditions, and after the seeds germinate, move them to the light for 12 hours a day, the light intensity is 1000lx, the culture temperature is 25°C, and the relative air humidity is 75%. After 30 days of cultivation, the germination rate reaches 100%. The seed germination medium is: MS+1.5mg / L 6-BA+NAA+25g / L sucrose+3.5g / L agar, with a pH of 5.5.

[0017] (2) Prolifera...

Embodiment 2

[0021] (1) Aseptic sowing of seeds: peel the seeds from the fruit and place them in a beaker, wash them with tap water, rinse them under tap water for 2 hours, and then rinse them with distilled water for 4 times. Place the rinsed seeds on an ultra-clean workbench, soak them in 75% ethanol for 10 s, and use 0.1% HgCl 2 Soak and disinfect for 8 minutes, and finally rinse with sterile water 5 times. The seed coats of the sterilized seeds are peeled off in a sterile petri dish to obtain mature seed embryos, which are then inoculated into a seed germination medium. After inoculation, cultivate under dark conditions, and after the seeds germinate, move them to the light for 13 hours a day, the light intensity is 1500lx, the culture temperature is 28°C, and the relative air humidity is 75%. After 30 days of cultivation, the germination rate reaches 98.5%. The seed germination medium is: MS+2.0mg / L 6-BA+NAA+30g / L sucrose+4.5g / L agar, with a pH of 5.6.

[0022] (2) Prolifer...

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Abstract

The invention discloses an establishment method of an S.Davidianavar. undulata. isolated culture regeneration system. S.Davidianavar. undulata. is a rosaceous stranvaesia evergreen dwarf shrub. The petals of the S.Davidianavar. undulata. are white, while the fruits of the S.Davidianavar. undulata. are orange; in autumn, the leaves of the S.Davidianavar. undulata. are vermeil, and the red leaf period lasts for one month; the fruits do not fall in winter; therefore, the S.Davidianavar. undulata. becomes incomparable rare trees species in ornamental flower-viewing, leaf-viewing, and fruit and flower-viewing shrubs in China at present. At present, the seedling propagation of the S.Davidianavar. undulata. is mainly performed in such manners of seed propagation and cutting propagation, and such manners have the problems of long period, low efficiency, high cost and the like, and fail in meeting the requirements of large-scale and standard production; as a result, the establishment of the S.Davidianavar. undulata. isolated culture regeneration system is necessary. According to the establishement method, the seed of the S.Davidianavar. undulata. is taken as an explant, an S.Davidianavar. undulata. in-vitro regenerated plant is obtained by virtue of the processes such as sowing, multiplication culture, rooting culture, and acclimatization and transplanting, and the S.Davidianavar. undulata. isolated culture regeneration system is established.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for establishing an in vitro culture and regeneration system of a red fruit tree. Background technique [0002] wave leaf red fruit tree ( S. Davidiana var. undulata. ) is an evergreen dwarf shrub of the genus Rosaceae. Its petals are white, the fruit is orange-red, and the leaves are vermilion in autumn. The red leaf period lasts for a month, and the fruit does not fall for a long time in winter. One of the tree species. It is distributed in Guangxi, Sichuan, Jiangxi, Zhejiang, Shaanxi, Hunan, Hubei and other provinces and regions in my country, mainly growing in hillsides, mountain tops, river valleys and shrubs at an altitude of 1300-1900m. [0003] At present, the seedling propagation of Boyehongguo tree is mainly carried out by seed propagation, cutting propagation, etc., which has problems such as long cycle, low efficiency, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 冯文杰
Owner 冯文杰
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