Method for separating and purifying phlorizin in crabapple (malus hupenhensis) water extract

Abstract

The invention provides a method for separating and purifying phlorizin in crabapple (malus hupenhensis) water extract. The method concretely comprises the following steps: concentrating the crabapplewater extract at 75-85 DEG C to obtain extractum; cooling the extractum to the room temperature, then transferring the cooled extractum into a refrigeration storage at 0-5 DEG C, standing and carryingout crystallization for 20-30h; evenly mixing the crystallized extractum with ice water, separating and filtering to remove mother liquor; after the mother liquor is filtered out, adding the ice water for washing, and spin-drying to obtain a product containing the phlorizin; dissolving the crude phlorizin in ethanol at the room temperature, filtering, concentrating under reduced pressure, and collecting paste; adding hot water, evenly mixing, then standing and cooling; after the temperature is lowered to the normal temperature, transferring the product into the refrigeration storage at 0-5 DEG C, standing, and crystallizing for 20-30h to obtain the pure phloridzin. The affordable, stable and reliable method for separating and purifying the phlorizin in the malus hupenhensis water extractis developed by using the common industrial equipment and solvent, and has the characteristics of being simple in process, safe and reliable, easy to implement, high in recovery rate, low in cost, andthe like.

Description

technical field [0001] The present invention relates to a method for separating and purifying phlorizin in Hubei crabapple water extract and its application in industrial production Background technique [0002] Phloridin was first extracted from apples, apple bark and leaves, etc. It is a flavonoid glycoside compound composed of aglycon phloretin and glucose, which belongs to dihydrochalcone glycosides. Current research shows that phlorizin has a unique effect on the prevention and treatment of diabetes and its complications, and has good anti-oxidation, anti-cancer, and whitening effects. [0003] Hubei crabapple (Malus hupehensis (Pamp.) Rehd.), produced in Hubei, Hunan, Jiangxi, Jiangsu, Zhejiang, Anhui, Fujian, Guangdong, Gansu, Shaanxi, Henan, Shanxi, Shandong, Sichuan, Yunnan, Guizhou and other places. Growing on hillsides or valleys in jungles, 50-2900 meters above sea level. Hubei Begonia contains phlorizin, and the content of each part varies from 0.9 to 13%. Amo...

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Problems solved by technology

[0005] Aiming at the shortcomings of many impurities, high viscosity and difficult separation in the water extract of crabapple from Hubei, the purpose of the present invention is to provide an industrial-grade method for separating and purifying phlorizin from the water extract of crabapple from Hubei, which has the advantages of simple process, safe Reliable, easy to implement, high recovery rate, low cost, etc.

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