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MdABCG gene promoter deletion fragment, and application thereof in detection of dwarfing of apple plants

A technology of apple genus and promoter, applied in the directions of DNA/RNA fragments, biochemical equipment and methods, recombinant DNA technology, etc., can solve the problem of weakened vegetative growth of trees, reduced auxin synthesis, insufficient supply of cytokinins in shoots, etc. question

Active Publication Date: 2020-04-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The dwarfing mechanism of apple dwarf rootstocks may be the result of restricted cytokinin transport between the root system and the shoot, that is, the up-transport of cytokinin in the root system of the dwarf rootstock is blocked, resulting in insufficient supply of cytokinin in the shoot, resulting in The vegetative growth of the tree is weakened, causing the reduction of auxin synthesis in the shoot

Method used

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  • MdABCG gene promoter deletion fragment, and application thereof in detection of dwarfing of apple plants
  • MdABCG gene promoter deletion fragment, and application thereof in detection of dwarfing of apple plants
  • MdABCG gene promoter deletion fragment, and application thereof in detection of dwarfing of apple plants

Examples

Experimental program
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Effect test

Embodiment 1

[0075] Example 1, Obtaining the Deleted Sequence of the Cytokinin Transporter Gene MdABCG Promoter

[0076] 1. MdABCG promoter sequence analysis

[0077] The cytokinin transporter gene MdABCG gene was searched in the apple genome, and it was found that there was only one MdABCG gene in the whole apple genome, which was located on chromosome 15, such as figure 1 shown. figure 1 It shows that the basic structure of the genome sequence of the MdABCG protein includes 5 exons and 4 introns. The position of A is recorded as +1, - represents the 5' direction of the MdABCG gene start codon ATG in the genome sequence of the MdABCG protein, and + represents the 3' direction of the MdABCG gene start codon ATG in the genome sequence of the MdABCG protein.

[0078] Two, the amplification of apple rootstock MdABCG gene promoter

[0079] The genomic DNAs of dwarf apple rootstock (M9) and arborizing apple rootstock (Balonia chinensis) were extracted, respectively, and PCR amplification was...

Embodiment 2

[0086] Example 2, Application of MdABCG Gene Promoter Deletion Fragment in Detection of Malus Rootstock Dwarfing Ability

[0087] 1. Detection of MdABCG gene promoter deletion fragment

[0088] 1. Genomic DNA of hybrid offspring with dwarf difference plants obtained

[0089] The hybrid offspring of the F1 generation was obtained by crossing with Begonia as the female parent and M9 as the male parent.

[0090] The leaves of the F1 generation of 10 plants (strain numbers 1-10) with dwarfing difference in the F1 generation hybrid progeny were collected respectively, and the genomic DNA was extracted.

[0091] 2. Detection of MdABCG gene promoter deletion fragment

[0092] Design and synthesize the following primers: (design an upstream primer about 200bp upstream of the "MdABCG gene promoter deletion fragment", and design a downstream primer downstream of the deletion fragment)

[0093] MdABCG-F: 5'-AAAGGTGGGATTCCCACCAT-3' (SEQ ID No.4);

[0094] MdABCG-R: 5'-TCCTGGGATGGAAGAT...

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Abstract

The invention discloses an MdABCG gene promoter deletion fragment, and an application thereof in detection of dwarfing of apple plants. A method for detecting the rootstock dwarfing capacity of appleplants, provided by the invention, comprises the following steps: detecting whether the genome of a to-be-detected apple plant contains the MdABCG gene promoter deletion fragment or not, and judging that the rootstock dwarfing capacity of the to-be-detected apple plant without the MdABCG gene promoter deletion fragment in the genome is higher than that of the to-be-detected apple plant with the MdABCG gene promoter deletion fragment in the genome, wherein the MdABCG gene promoter deletion fragment is from the -64th site to the -25th site in the upstream direction of A in an apple plant genomeMdABCG gene initiation codon ATG. The MdABCG gene promoter fragment deletion phenomenon is positively related to dwarfing traits, dwarfing plants in offspring can be rapidly screened by utilizing deletion and existence of the fragment, and the MdABCG gene promoter deletion fragment has great significance in the field of dwarfing of plants.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a missing fragment of an MdABCG gene promoter and its application in detecting the dwarfing of Malus plants. Background technique [0002] Dwarfing and dense planting of apple has the advantages of early fruiting, good quality, convenient management, fast variety update and high economic benefit, and has become the trend of modern apple production and development. So far, the utilization of dwarfing rootstocks is still the main way to realize the dwarfing and dense planting of apples. [0003] The dwarfing mechanism of apple dwarf rootstocks may be the result of restricted cytokinin transport between the root system and the shoot, that is, the up-transport of cytokinin in the root system of the dwarf rootstock is blocked, resulting in insufficient supply of cytokinin in the shoot, resulting in The vegetative growth of the tree is weakened, which causes the reduction of auxin synthesis...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 韩振海冯轶张桂粉王忆许雪峰张新忠吴婷
Owner CHINA AGRI UNIV
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