Extraction and detection method of 2-O-beta-D-glucopyranosyl-L-ascorbic acid from fructus malus toringoides and fructus malus tiansitoria

A detection method and technology of medlaric acid, which are applied in chemical instruments and methods, measuring devices, preparation of sugar derivatives, etc., can solve the problems such as unseen russian medlaric acid, etc., and achieve the effect of good application prospect.

Active Publication Date: 2019-11-05
SICHUAN ZHIJIACHENG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report about Lycium acid contained in Russian fruit

Method used

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  • Extraction and detection method of 2-O-beta-D-glucopyranosyl-L-ascorbic acid from fructus malus toringoides and fructus malus tiansitoria
  • Extraction and detection method of 2-O-beta-D-glucopyranosyl-L-ascorbic acid from fructus malus toringoides and fructus malus tiansitoria
  • Extraction and detection method of 2-O-beta-D-glucopyranosyl-L-ascorbic acid from fructus malus toringoides and fructus malus tiansitoria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The preparation of embodiment 1 Russian fruit extract

[0067] Take 700g of Eseguo powder (passed through a No. 4 sieve), ultrasonically extract twice with ten times the amount of pure water, each time for 1 hour, combine the extracts to about 15L, after the extracts stand still, take the supernatant and concentrate to 2L at 50°C , the concentrated solution is filtered with 200-300 mesh silica gel, and then filtered with a 0.45 μm micromembrane as the preparation solution,

[0068] Take the above preparation solution and prepare it by high performance liquid chromatography, using C18 chromatographic column, the detection wavelength is 235nm, using methanol: 0.1% phosphoric acid water = 4:96v / v as the eluent in turn, and the flow rate is 0.5 ~ 1.0ml / min. Elution, take the fraction of 13-20min to get the extract of russa fruit, the purity is greater than 80%, the HPLC detection spectrum is shown in figure 1 , the peak with a retention time of 14.727 is the target peak. ...

Embodiment 2

[0069] The preparation method of wolfberry acid in embodiment 2, Russia color fruit

[0070] The Russian fruit extract that embodiment 1 obtains is used as the sample section;

[0071] Take the above sample section, and use the preparative high performance liquid chromatography (chromatographic column: ultimate XB-ODS, 4.6*250mm, 5 μm; Mobile phase: A-methanol B-0.1% phosphoric acid solution; Detection wavelength: UV-235nm; Flow rate: 1.0 mL / min; column temperature: 30°C; see the following table for gradient elution procedure See the detection map figure 2 .

[0072] Table 1. Gradient elution program

[0073] time (min) Mobile phase A(%) Mobile phase B(%) 0～15 1 99 15～25 1→10 99→90 25～30 10→5 90→95 30～40 5 95 40～50 5→1 95→99

[0074] The DAD scan of the target peak is shown in image 3 , see mass spectrometric identification Figure 4 , Figure 5 , see NMR Image 6 , Figure 7 , Figure 8 , Figure 9 , Figure 10 . ...

Embodiment 3

[0084] Embodiment 3, the HPLC content determination of lycitric acid in Russian fruit extract and Russian fruit

[0085] 1. Drugs

[0086] Seventeen batches of Russian fruits were collected, all of which were collected from Luhuo County, Ganzi Prefecture, Sichuan Province. They were identified as Malus toringoides (Rehd.) Hughes and Malus transitoria (Batal. ) Dried ripe fruit of Schneid.

[0087] According to Example 1, 5 batches of russian fruit extract were prepared.

[0088] 2. Reagents and drugs

[0089] Pure water; Methanol (chromatographically pure); Acetonitrile (chromatographically pure); Phosphoric acid (analytical pure);

[0090] 3. Experimental equipment

[0091] KH-500V ultrasonic cleaner; Agilent technologies 1200 high performance liquid chromatography (Agilent Technologies Co., Ltd., USA); SQP electronic analytical balance (Sartorius Scientific Instruments Co., Ltd.).

[0092] 3. Detection method

[0093] 1) Preparation of reference substance

[0094] Acc...

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Abstract

The invention discloses an extract of fructus malus toringoides and fructus malus tiansitoria and a preparation and detection method of 2-O-beta-D-glucopyranosyl-L-ascorbic acid from the fructus malustoringoides and fructus malus tiansitoria. Experimental results show that the 2-O-beta-D-glucopyranosyl-L-ascorbic acid is successfully separated from the fructus malus toringoides and fructus malustiansitoria, and is discovered in the fructus malus toringoides and fructus malus tiansitoria for the first time. Therefore, the 2-O-beta-D-glucopyranosyl-L-ascorbic acid can be used for the identification and quality control over the fructus malus toringoides and fructus malus tiansitoria, and a foundation is laid for the excavation of pharmacodynamic effects of the fructus malus toringoides andfructus malus tiansitoria.

Description

technical field [0001] The invention relates to an extraction method and a detection method of wolfberry acid in Russian fruit. Background technique [0002] "Russie", also known as "Wado" (Tibetan), comes from Malustoringoides (Rehd.) Hughes and Malus tiansitoria (Batal.) Schneid, the Rosaceae plants of the genus Malustoringoides (Rehd.) Schneid. Lowering blood pressure, curing liver disease, diarrhea, eye disease, irregular menstruation, etc., is a commonly used folk medicine in the Tibetan area of ​​Ganzi Prefecture, and has a long history of medicinal and edible use. Modern research shows that Russian fruit is rich in vitamin C and vitamin B2; fructose, glucose, disaccharides, polysaccharides, etc.; myristic acid, palmitic acid, palmitoleic acid, oleic acid, linoleic acid, linolenic acid and other fatty acids and proteins However, there are no clear and systematic research reports on its physical and chemical components. [0003] Lycium acid, English name: 2-O-β-D-Gluc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08G01N30/06G01N30/02
CPCC07H17/04C07H1/08G01N30/06G01N30/02Y02A50/30
Inventor 何刚
Owner SICHUAN ZHIJIACHENG BIO TECH CO LTD
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