mdabcg Gene Promoter Deletion Fragment and Its Application in Detection of Malus Plant Dwarfing

A technology of apple genus and promoter, applied in the directions of DNA/RNA fragments, biochemical equipment and methods, recombinant DNA technology, etc., can solve the shortage of cytokinin supply in the shoot, the reduction of auxin synthesis, and the weakened vegetative growth of trees, etc. question

Active Publication Date: 2022-02-18
CHINA AGRI UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The dwarfing mechanism of apple dwarf rootstocks may be the result of restricted cytokinin transport between the root system and the shoot, that is, the up-transport of cytokinin in the root system of the dwarf rootstock is blocked, resulting in insufficient supply of cytokinin in the shoot, resulting in The vegetative growth of the tree is weakened, causing the reduction of auxin synthesis in the shoot

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • mdabcg Gene Promoter Deletion Fragment and Its Application in Detection of Malus Plant Dwarfing
  • mdabcg Gene Promoter Deletion Fragment and Its Application in Detection of Malus Plant Dwarfing
  • mdabcg Gene Promoter Deletion Fragment and Its Application in Detection of Malus Plant Dwarfing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1, Obtaining the Deleted Sequence of the Cytokinin Transporter Gene MdABCG Promoter

[0075] 1. MdABCG promoter sequence analysis

[0076] The cytokinin transporter gene MdABCG gene was searched in the apple genome, and it was found that there was only one MdABCG gene in the whole apple genome, which was located on chromosome 15, such as figure 1 shown. figure 1 It shows that the basic structure of the genome sequence of the MdABCG protein includes 5 exons and 4 introns. The position of A is recorded as +1, - represents the 5' direction of the MdABCG gene start codon ATG in the genome sequence of the MdABCG protein, and + represents the 3' direction of the MdABCG gene start codon ATG in the genome sequence of the MdABCG protein.

[0077] Two, the amplification of apple rootstock MdABCG gene promoter

[0078] The genomic DNAs of dwarf apple rootstock (M9) and arborizing apple rootstock (Balonia chinensis) were extracted, respectively, and PCR amplification was...

Embodiment 2

[0085] Example 2, Application of MdABCG Gene Promoter Deletion Fragment in Detection of Malus Rootstock Dwarfing Ability

[0086] 1. Detection of MdABCG gene promoter deletion fragment

[0087] 1. Genomic DNA of hybrid offspring with dwarf difference plants obtained

[0088] The hybrid offspring of the F1 generation was obtained by crossing with Begonia as the female parent and M9 as the male parent.

[0089] The leaves of the F1 generation of 10 plants (strain numbers 1-10) with dwarfing difference in the F1 generation hybrid progeny were collected respectively, and the genomic DNA was extracted.

[0090] 2. Detection of MdABCG gene promoter deletion fragment

[0091] Design and synthesize the following primers: (design an upstream primer about 200bp upstream of the "MdABCG gene promoter deletion fragment", and design a downstream primer downstream of the deletion fragment)

[0092] MdABCG-F: 5'-AAAGGTGGGATTCCCACCAT-3' (SEQ ID No.4);

[0093] MdABCG-R: 5'-TCCTGGGATGGAAGAT...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an MdABCG gene promoter deletion fragment and its application in detecting the dwarfing of Malus plants. The invention provides a method for detecting the dwarfing ability of rootstocks of plants of the genus Malus, comprising: detecting whether the genome of plants of the genus Malus contains a missing fragment of the MdABCG gene promoter; The rootstock dwarfing ability of the genus plant is higher than that of the tested Malus plant containing the MdABCG gene promoter deletion fragment in the genome; the MdABCG gene promoter deletion fragment is the upstream of A in the MdABCG gene initiation codon ATG of the Malus plant genome Direction ‑64th to ‑25th. The absence of the MdABCG gene promoter fragment is positively correlated with dwarfing traits, and the absence and presence of the fragment can be used to quickly screen dwarf plants in offspring, which is of great significance in the field of plant dwarfism.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a missing fragment of an MdABCG gene promoter and its application in detecting the dwarfing of Malus plants. Background technique [0002] Dwarfing and dense planting of apple has the advantages of early fruiting, good quality, convenient management, fast variety update and high economic benefit, and has become the trend of modern apple production and development. So far, the utilization of dwarfing rootstocks is still the main way to realize the dwarfing and dense planting of apples. [0003] The dwarfing mechanism of apple dwarf rootstocks may be the result of restricted cytokinin transport between the root system and the shoot, that is, the up-transport of cytokinin in the root system of the dwarf rootstock is blocked, resulting in insufficient supply of cytokinin in the shoot, resulting in The vegetative growth of the tree is weakened, which causes the reduction of auxin synthesis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 韩振海冯轶张桂粉王忆许雪峰张新忠吴婷
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap