Bacillus thuringiensis, microecological preparation for improving livestock and poultry breeding environment and application of microecological preparation

Abstract

The invention discloses bacillus thuringiensis, a microecological preparation for improving a livestock and poultry breeding environment and application of the microecological preparation. The bacillus thuringiensis is bacillus thuringiensis HY-002 and is preserved in the China Center for Type Culture Collection (CCTCC), the number of the bacillus thuringiensis is CCTCC NO: M20242390, and the preservation date of the bacillus thuringiensis is October 31, 2024. The microecological preparation is prepared from the following three kinds of probiotics: bacillus subtilis HY-001 (1-8) * 10 < 9 > CFU / g, bacillus licheniformis (1-8) * 10 < 9 > CFU / g and bacillus thuringiensis HY-002 (1-5) * 10 < 8 > CFU / g. According to the method, the mode of killing the mosquitoes and the mode of reducing the ammonia nitrogen odor of the breeding environment are combined, the aim of reducing the mosquitoes is finally achieved, and therefore the occurrence of viruses or bacterial diseases of bred animals is reduced, the breeding environment is improved, and the animal living environment welfare is improved. The microecological preparation can be applied to livestock and poultry animal breeding environment spraying, excrement compost for reducing mosquitoes and flies, manure pits and other environments with many mosquitoes and odor, can be directly dissolved in water and diluted according to the ratio of 1: 1000 and then sprayed to the environment, and has the advantages of being safe, convenient to use and efficient.

Description

Technical Field

[0001] The invention relates to Bacillus thuringiensis, and also relates to a microecological preparation which can effectively improve the livestock and poultry breeding environment by using the Bacillus thuringiensis, and the application of the microecological preparation. Background Art

[0002] Bacillus thuringiensis (Bt) is a Gram-positive bacterium. It is mainly used as a low-toxic insecticide from microorganisms to control a variety of pests such as Lepidoptera, Coleoptera, Hymenoptera, Diptera, and Orthoptera. Its insect control principle is that the strain can produce two types of toxins, endotoxins (paraspore crystals) and exotoxins, which stop pests from feeding. Pests die of starvation, blood corruption, and neurotoxicity. Further research has found that the existing Bacillus thuringiensis has a toxic effect on more than 200 pests of Lepidoptera, but the control effect on mosquitoes and flies of Diptera is not ideal.

[0003] As a place where animals are concentrated, livestock and poultry farms are prone to outbreaks of animal viral or bacterial diseases, and the common transmission media are mainly mosquitoes, flies, etc. Therefore, if the number of mosquitoes and flies can be effectively reduced, the spread of diseases can be effectively cut off to a certain extent, thereby reducing the occurrence of animal diseases. However, livestock and poultry farms inevitably produce odors and peculiar smells due to the accumulation of feces, which makes it easier to breed or attract mosquitoes and flies.

[0004] Therefore, the present invention is aimed at the environment of livestock and poultry breeding sites, and specially develops a microecological preparation that uses special Bacillus thuringiensis as insecticide, and special Bacillus subtilis and Bacillus licheniformis as degrading environmental odor. The two are compounded and complement each other, and can effectively remove the odor of the breeding environment and kill mosquitoes and flies, thereby improving the environment of the breeding site and improving the welfare of the animal living environment. Summary of the invention

[0005] In order to improve the environment of livestock and poultry breeding sites, the present invention provides a Bacillus thuringiensis, and also provides a microecological preparation capable of killing mosquitoes and reducing the odor of breeding environment and its application.

[0006] In order to achieve the above object, the solution of the present invention is:

[0007] A Bacillus thuringiensis HY-002, deposited in the China Center for Type Culture Collection (CCTCC), with a deposit number of CCTCC NO: M 20242390 and a deposit date of October 31, 2024.

[0008] The Bacillus thuringiensis HY-002 is isolated from soil: fertile soil from fallen leaves under trees is added into sterile physiological saline for mixing and shaking, non-spore bacteria are killed by water bath, and the soil is sprayed on the surface of fruits after cooling to feed fruit flies; multiple dead fruit flies are selected, their abdomens are opened respectively, and strains are separated by streaking using an inoculation loop; different dead fruit flies are streaked out, and colonies with similar appearance are separated and purified by streaking again; thus, Bacillus thuringiensis HY-002 is obtained.

[0009] A microecological preparation for improving the livestock and poultry breeding environment, comprising the following three probiotics: Bacillus subtilis (1-8) × 10 9 CFU / g, Bacillus licheniformis (1-8)×10 9 CFU / g, Bacillus thuringiensis (1-5)×10 8 CFU / g, where

[0010] The Bacillus subtilis is Bacillus subtilis HY-001, which is a strain with patent number ZL202210872869.X, and is derived from the China Center for Type Culture Collection, with strain number CCTCC NO.M2022622;

[0011] The Bacillus licheniformis is from Guangdong Microbiological Culture Collection Center, and the strain number is GDMCC1.11;

[0012] The Bacillus thuringiensis is the aforementioned Bacillus thuringiensis HY-002.

[0013] Among them, the test of insecticide of the Bacillus thuringiensis HY-002 found that it has a killing effect on mosquito larvae, fruit flies and second-instar silkworms, and the killing effect is ranked as follows: Bacillus thuringiensis HY-002 bacteria> fermentation liquid> supernatant. The Bacillus thuringiensis HY-002 has been verified by experiments to have a good effect of killing mosquitoes and flies.

[0014] The Bacillus subtilis HY-001 can survive and carry out normal growth and metabolism in an environment of 50-55° C., and can produce a large amount of highly efficient cellulase and amylase, and has a significant effect in composting applications.

[0015] The microecological preparation for improving the livestock and poultry breeding environment has a carrier of maltodextrin, which can be completely dissolved in water and will not clog the nozzle.

[0016] The microecological preparation for improving the livestock and poultry breeding environment can be used for spraying in livestock and poultry breeding environments, composting manure to reduce mosquitoes and flies, and manure pits and other environments with many mosquitoes and odors. It can be directly diluted in water at a ratio of 1:1000 and then sprayed in the environment. It is safe, convenient and efficient.

[0017] The microecological preparation for improving the livestock and poultry breeding environment of the present invention has the following advantages:

[0018] 1. Bacillus thuringiensis HY-002 has a good effect on killing mosquitoes and can effectively reduce the number of mosquitoes in the breeding environment.

[0019] 2. Bacillus subtilis HY-001 can produce a large amount of highly efficient cellulase and amylase and when combined with Bacillus licheniformis, it can effectively reduce ammonia nitrogen and odor in the breeding environment, thereby helping to reduce the breeding of mosquitoes.

[0020] 3. By killing mosquitoes and reducing ammonia nitrogen in the breeding environment, the two complement each other, promote the improvement of the livestock and poultry breeding environment, and are beneficial to the health of farmed animals.

[0021] Fourth, the traditional methods of killing or repelling mosquitoes mostly use chemical drugs or physical mosquito repellent lamps. Chemical drugs are not environmentally friendly, and mosquito repellent lamps need to consider the power supply, which is relatively cumbersome. The microecological preparation of the present invention can be directly dissolved in water and then sprayed into the environment, which is safe, convenient and efficient.

[0022] In summary, the present invention mainly combines killing mosquitoes with reducing ammonia nitrogen odor in the breeding environment, ultimately achieving the purpose of reducing mosquitoes, thereby reducing the occurrence of viral or bacterial diseases in farmed animals, improving the breeding environment, and improving the welfare of animal living environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is the growth curve of Bacillus thuringiensis HY-002 at different temperatures;

[0024] Figure 2 The feeding status of silkworms on the 0th day after adding the test substance and on the 2nd day after replacing new mulberry leaves;

[0025] Figure 3 Five days after the test substances were added to the silkworms, there were no deaths among the three experimental groups and no deaths in the control group;

[0026] Figure 4 0 day after adding the test substance to D. melanogaster;

[0027] Figure 5 Four days after adding the test substance to Drosophila melanogaster, a large number of dead fruit flies appeared at the bottom of the bottle;

[0028] Figure 6 Four days after adding the test substance to Drosophila melanogaster, a large number of dead fruit fly bodies and bacterial groups appeared at the bottom of the bottle. DETAILED DESCRIPTION

[0029] In order to further describe the technical solution of the present invention, the following specific embodiments are made, but the content of the present invention is not limited to the scope described in the embodiments. All that does not deviate from the concept of the present invention and equivalent substitutions are within the protection scope of the present invention.

[0030] Example 1 Screening of Bacillus thuringiensis HY-002

[0031] Take 10g of fertile soil from fallen leaves under the tree, add it to 90mL of sterile saline, mix and shake for 10 minutes, and kill non-spore bacteria in a water bath at 80℃ for 10 minutes. After cooling, spray the surface of the fruit and feed it to fruit flies. Select several dead fruit flies, pick open their abdomens, and use an inoculation loop to separate the strains by streaking. Select different dead fruit flies and streak them out. Colonies with similar appearance are separated and purified by streaking again. Smear the purified strain on the surface of the fruit and feed it to fruit flies to verify whether it can kill the fruit flies, and then send it for strain identification.

[0032] After screening tests, a strain was screened and sequenced by 16S rRNA. The obtained sequence was compared online with the BLAST database of the National Center for Biotechnology Information (NCBI). The results showed that the gene series of the strain was 100% homologous to Bacillus thuringiensis.

[0033] Using 16S rRNA sequencing, the fragment length obtained was 1427 bp. The specific sequencing series results were:

[0034]

[0035] Thus, the Bacillus thuringiensis HY-002 in the microecological preparation for improving the livestock and poultry breeding environment of the present invention is obtained and preserved in the China Center for Type Culture Collection (CCTCC) with a preservation number of CCTCC NO: M 20242390 and a preservation date of October 31, 2024.

[0036] Example 2 Screening of the Optimal Fermentation Temperature for Bacillus thuringiensis HY-002

[0037] The Bacillus thuringiensis HY-002 of Example 1 was inoculated into a nutrient broth medium, cultured at different temperatures for 24 hours, and the number of viable bacteria was detected to test the optimal growth temperature.

[0038] Table 1

[0039]

[0040] From the data in Table 1 and Figure 1It can be seen that the optimal growth temperature of Bacillus thuringiensis HY-002 is 25-30℃, and this temperature is relatively close to the ambient temperature, so the bacteria is suitable for growth at ambient temperature.

[0041] Example 3 Liquid fermentation culture of Bacillus thuringiensis HY-002 and pretreatment of bacterial liquid

[0042] 1. Liquid Fermentation Culture

[0043] The Bacillus thuringiensis HY-002 of Example 1 was inoculated into a nutrient broth medium and cultured at 28°C for 24 h. The viable bacterial count was found to be 1.1×10 8 CFU / mL, pH 6.55.

[0044] 2. Preparation of test substances by pretreatment of fermentation liquid

[0045] Take 50 mL of the fermented bacterial suspension (containing fermented liquid and bacterial cells) directly for later use, and take another 50 mL of the fermented bacterial suspension and centrifuge it at 4000 r / min for 10 min, collect 50 mL of supernatant (fermented liquid without bacterial cells) for later use. For bacterial precipitate, resuspend it with 50 mL of sterile water and centrifuge it again, remove the supernatant and resuspend it with 50 mL of sterile water (collect only bacterial cells) for later use.

[0046] Example 4 Insecticidal test of Bacillus thuringiensis HY-002

[0047] 1. Test on second-instar silkworms

[0048] Referring to Part 3, Silkworm Toxicity Test, NY / T3152.3-2017 "Test Guidelines for Environmental Risk Assessment of Microbial Pesticides" drafted by the Pesticide Control Institute of the Ministry of Agriculture and the Nanjing Institute of Environmental Sciences of the Ministry of Ecology and Environment, toxicity tests were conducted and corresponding modifications were made.

[0049] In this study, 1.1×10 8 CFU / mL was used as the maximum harmful dose for the maximum harmful dose test. The test method adopted the leaf immersion method, and 50mL of the test substance in Example 3 was used to soak 10g of fresh mulberry leaves (remove the thick petiole) for 1 minute to evenly distribute the test substance on the leaves. After taking out and drying in the shade, they were evenly placed in 3 culture dishes with a diameter of 20cm. 20 second-instar silkworms were inoculated into each culture dish. After feeding for 24 hours, they were fed with clean mulberry leaves without the test substance (unlimited). There were 3 parallels in each group, with 20 silkworms in each parallel. Each group of silkworms was raised at a temperature of 25°C and a relative humidity of 70-85%. Among them, the control group used sterile water instead of the test substance, and the other steps were the same. Taking into account the infectivity of microorganisms, cross contamination should be avoided in each treatment group and the control group during the test.

[0050] After the experiment began, the number of deaths, poisoning symptoms and other abnormal behaviors of the tested silkworms were observed and recorded. The criterion for judging death was that the insect body did not move when lightly touched with tweezers.

[0051] Table 2

[0052]

[0053]

[0054] After the experiment was carried out for the second day, the silkworms in the control group ate normally, while the silkworms in the other three experimental groups showed symptoms of not eating and not moving, but no silkworms died on the second day. On the third day, silkworms began to die one after another. From Table 2 and Figure 2 , Figure 3 It can be seen that when the test was carried out to the fifth day, all 20 silkworms in a test group containing only the bacteria died, with a mortality rate of 100%, while the mortality rate of the fermentation liquid group was 90%, and the mortality rate of the supernatant group without bacteria was 70%, and no death occurred in the blank control group. This shows that after the fermentation of Bacillus thuringiensis HY-002 in Example 1, whether it is the bacteria itself (endotoxin) or a certain fermentation metabolite (exotoxin), it can kill the silkworms, and the lethal effect of bacteria>fermentation liquid>supernatant.

[0055] 2. Test on Drosophila melanogaster

[0056] Cut the potatoes into slices of uniform size and soak them in 50 mL of the test substance in Example 3 for 1 min. Evenly distribute the test substance on the cut surface of the potatoes, take them out and put them into 3 transparent cylindrical bottles. Inoculate 50 adult Drosophila melanogaster into each bottle and culture them in an incubator at 25°C. Dig a small hole at the mouth of the bottle to ensure air circulation. Each group has 3 parallels, with 50 Drosophila melanogaster in each parallel. The control group uses sterile water instead of the test substance, and the other steps are the same.

[0057] After the experiment begins, the death toll, poisoning symptoms and other abnormal behaviors of the tested Drosophila melanogaster are observed and recorded. The criterion for judging death is that the insect body does not move when lightly touched with tweezers.

[0058] Table 3

[0059] Experimental groups Number of fruit flies Number of deaths after 4 days mortality rate% Blank control group 50 3 6％ Bacteria 50 47 94％ Supernatant 50 21 42％ Fermentation Broth 50 32 64％

[0060] From Table 3 and Figure 4 , 56, when the experiment was carried out to the fourth day, 47 of the 50 fruit flies died in the test group containing only bacteria, with a mortality rate of 94%, while the mortality rate of the fermentation liquid group was 64%, and the mortality rate of the supernatant group without bacteria was only 42%, while the mortality rate of the blank control group was 6%. This shows that after the fermentation of Bacillus thuringiensis HY-002, whether it is the bacteria itself (endotoxin) or some fermentation metabolites (exotoxin), it can kill fruit flies, and the lethal effect of bacteria> fermentation liquid> supernatant.

[0061] 3. Test for mosquito larvae

[0062] Mosquito larvae (wrigg larvae) were placed in transparent cylindrical bottles, with 100 larvae of the same size and activity level in each bottle. 200 mL of clean water and 1 mL of the test substance in Example 3 were added respectively, with 3 parallels in each group. The control group used sterile water instead of the test substance, and the other steps were the same.

[0063] Maintain the temperature at around 25°C, place the insects on a safe platform, observe and record the number of larval deaths to calculate the kill rate. Use tweezers to gently touch the insect body and see if it does not move, which is used as the standard for judging death.

[0064] Table 4

[0065] Experimental groups Number of chironomid larvae Number of deaths after 3 days mortality rate% Blank control group 100 12 12％ Bacteria 100 95 95％ Supernatant 100 90 90％ Fermentation Broth 100 92 92％

[0066] As shown in Table 4, when the test was carried out to the third day, a large number of dead mosquito larvae appeared in all three experimental groups, with a mortality rate of 90-95%, and there was no significant difference among the three groups. At the same time, the mortality rate of the blank control group was 12%. This shows that after the fermentation of Bacillus thuringiensis HY-002, whether it is the bacteria itself (endotoxin) or a certain fermentation metabolite (exotoxin), it can kill mosquito larvae, and the difference in lethal effect is not significant.

[0067] Example 5 Comparison of insecticidal efficacy of different Bacillus thuringiensis

[0068] Two strains of Bacillus thuringiensis purchased from a strain collection institution: Bacillus thuringiensis (strain number GDMCC 1.144, from Guangdong Provincial Microbial Culture Collection Center) and Bacillus thuringiensis (strain number CICC22945, from China Industrial Microbial Culture Collection Management Center) were used to compare the insecticidal function with Bacillus thuringiensis HY-002 screened in Example 1 of the present invention. Silkworms, fruit flies and mosquito larvae were used for testing, and the test method and steps were the same as in Example 4 to verify whether there was a difference in the insecticidal efficacy of Bacillus thuringiensis from different sources.

[0069] Table 5

[0070] Experimental groups Mortality of silkworms after 5 days (%) Fruit fly mortality after 4 days (%) Mosquito larvae mortality rate after 3 days (%) Bacteria HY-002 of the present invention 100 95 96 GDMCC 1.144 86 0 0 CICC 22945 92 0 0

[0071] From the analysis of the test results in Table 5, it can be seen that the two purchased Bacillus thuringiensis strains only have insecticidal effects on silkworms, but have no insecticidal effects on fruit flies and mosquito larvae, while the Bacillus thuringiensis HY-002 screened by the present invention has good insecticidal effects on the three. This shows that even if it is the same Bacillus thuringiensis, but from different sources, it has differences in insecticidal function.

[0072] Example 6 Safety Test of Bacillus thuringiensis HY-002

[0073] 10 mice of the same size and health status and 10 chicks that had just hatched for 1 week were selected. While feeding them normally, Bacillus thuringiensis HY-002 of Example 1 was added to their daily drinking water, and the final content of the number of live bacteria in the drinking water was about 1.0×10 8 CFU / mL. After adding drinking water containing Bacillus thuringiensis HY-002 for 1 week, the water was changed to normal drinking water and then fed normally for another month. During this period, the health of mice and chickens was observed.

[0074] At the same time, a sterile circular filter paper was pasted on an agar plate containing 5% rabbit blood, 50 μL of Bacillus thuringiensis HY-002 bacterial solution was inoculated on the sterile circular filter paper, and aerobically cultured at 28° C. for 48 h to observe whether a hemolytic zone was formed.

[0075] In animal experiments, mice and chickens fed with Bacillus thuringiensis HY-002 behaved normally within one month, and no deaths or lesions occurred, indicating that Bacillus thuringiensis HY-002 is not pathogenic to mice and chickens. And after hemolytic tests, Bacillus thuringiensis HY-002 is not hemolytic to rabbit blood.

[0076] Example 7 Preparation of a microecological preparation for improving livestock and poultry breeding environment

[0077] A series of test combinations were prepared by compounding the live bacterial counts of three strains of Bacillus subtilis HY-001, Bacillus licheniformis and Bacillus thuringiensis HY-002 in different proportions, and the carrier was maltodextrin. In order to highlight the indispensability of the Bacillus subtilis HY-001 (CCTCC NO.M 2022622) of the present invention, a control combination was made with commercially available Bacillus subtilis. The experimental Bacillus licheniformis was derived from the Guangdong Provincial Microbial Culture Collection Center, and the strain number was GDMCC1.11; other commercially available Bacillus licheniformis can also be selected.

[0078] Table 6 Unit: CFU / g

[0079]

[0080] Example 8 Cattle breeding site test application

[0081] In a cattle breeding field (except for the middle aisle which is cement ground, the place where the cattle stay is land), 8 breeding sheds which are far apart from each other to avoid mutual interference were randomly selected for test. The 8 breeding sheds were respectively used with the implementation combination 1-5 and the control combination 1-3 prepared in Example 7. The method of use is to directly dissolve in water and dilute it at 1:1000 times and spray it in the breeding shed environment once a day. After continuous spraying for 3 days, stop spraying and observe the number of flies and mosquitoes in the environment and the change of odor. The management and feeding mode are not changed.

[0082] When the test time reached the 5th day, the field environment conditions were significantly different from those on the 1st and 2nd days, as shown in Table 7, and other breeding sheds that did not use the composition of Example 7 were used as blank controls.

[0083] Table 7

[0084]

[0085]

[0086] According to sensory observation, in terms of the effect on flies, mosquitoes and deodorization, the best effect is achieved by implementation combinations 1-2. When the content of Bacillus thuringiensis HY-002 is reduced (implementation combination 3), the effect of removing flies and mosquitoes is reduced, especially when it is not included (control combination 1). Bacillus subtilis and Bacillus licheniformis also have certain effects in deodorization. When they are not included (control combination 2 or implementation combination 5) and when the content is reduced (implementation combination 4), the deodorization effect is reduced. When the Bacillus subtilis HY-001 of the present invention is replaced by commercially available Bacillus subtilis (control combination 3), the deodorization effect is also poor, indicating that the Bacillus subtilis HY-001 of the present invention is indispensable.

[0087] Example 9: Test and Application of Broiler Farming Site

[0088] In a broiler breeding field (cement floor, net bed frame, broiler breeding bed frame), 7 breeding sheds that are far apart from each other to avoid mutual interference were randomly selected for test. The 7 breeding sheds were respectively used with the implementation combination 1-5 and the control combination 1-2 prepared in Example 7. The method of use is to directly dissolve in water and dilute it at 1:1000 times and spray the breeding shed environment, especially the feces on the ground under the net bed frame, once a day. After continuous spraying for 3 days, stop spraying and observe the number of flies and mosquitoes in the environment and the change of odor. The management and feeding mode are not changed.

[0089] When the test time reached the 6th day, the field environment conditions were significantly different from those on the 1st and 2nd days, as shown in Table 8, and other breeding sheds that did not use the composition of Example 7 were used as blank controls.

[0090] Table 8

[0091]

[0092]

[0093] Since the broiler breeding ground is cement ground, unlike the mud ground in Example 8, the mud ground can be used as a probiotic fermentation bed, while the probiotics on cement ground are difficult to attach and are generally cleaned up with the cleaning of the floor feces, so the deodorization effect of this test is greatly reduced. However, after the feces are removed, there is still a clear difference through sensory observation.

[0094] As for the effect of removing flies, mosquitoes and odors, the effect of implementation combination 1-2 is still the best. When the content of Bacillus thuringiensis HY-002 is reduced (implementation combination 3), the effect of removing mosquitoes is reduced, especially when it is not contained (control combination 1). Bacillus subtilis and Bacillus licheniformis also have a certain effect in deodorization. Although it is difficult for probiotics to attach to cement floors, probiotics still attach to the net bed frame during environmental spraying, which also plays a certain role in deodorization.

[0095] Example 10: Feces composting test application

[0096] In the compost of chicken manure, the three samples of implementation combination 1, control combination 1 and control combination 2 in Example 7 were mixed at a ratio of 1 kg / ton of compost. Each pile was controlled to have 5 tons of material, a height of 1 meter, and a composting period of 30 days. The flies and odor of the compost were observed, and samples were taken every 5 days in the early stage of composting to detect relevant indicators and record relevant test data, among which the fecal coliform group number was detected according to GB / T19524.1, and the ascaris egg mortality rate was detected according to GB / T19524.2. At the same time, the control group was not treated.

[0097] Table 9

[0098]

[0099]

[0100] In the first 15 days of feces composting, the implementation combination 1 of the microecological preparation of the present invention can effectively reduce the number of flies and fecal coliforms, quickly reduce environmental odor, and increase the mortality rate of ascarid eggs, thereby shortening the composting time. In the control combination 1 without Bacillus thuringiensis HY-002, because Bacillus subtilis HY-001 can quickly increase the composting temperature, the feces can be quickly fermented and matured, but the number of flies is still more than the implementation combination 1. In the control combination 2 with Bacillus thuringiensis HY-002 and no Bacillus subtilis HY-001, although the composting maturity effect is poor and the odor is heavy, the number of flies is still not significantly increased. It shows that Bacillus thuringiensis HY-002 has a certain effect on reducing the number of flies in the feces composting process, and its compounding effect with Bacillus subtilis HY-001 is better.

[0101] Part of the embodiments of the present invention are described above, but the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0102]

Claims

1. A Bacillus thuringiensis, characterized in that: It is Bacillus thuringiensis HY-002, deposited in the China Center for Type Culture Collection CCTCC, with the deposit number CCTCC NO: M 20242390, and the deposit date is October 31, 2024.

2. The Bacillus thuringiensis according to claim 1, characterized in that: The Bacillus thuringiensis HY-002 is isolated from soil: fertile soil from fallen leaves under trees is added into sterile physiological saline for mixing and shaking, non-spore bacteria are killed by water bath, and the soil is sprayed on the surface of fruits after cooling to feed fruit flies; multiple dead fruit flies are selected, their abdomens are opened respectively, and strains are separated by streaking using an inoculation loop; different dead fruit flies are streaked out, and colonies with similar appearance are separated and purified by streaking again; thus, Bacillus thuringiensis HY-002 is obtained.

3. A microecological preparation for improving the livestock and poultry breeding environment, characterized in that It is composed of the following three probiotics: Bacillus subtilis (1-8) × 10 9 CFU / g, Bacillus licheniformis (1-8)×10 9 CFU / g, Bacillus thuringiensis (1-5)×10 8 CFU / g, where The Bacillus subtilis is Bacillus subtilis HY-001, which is from China Center for Type Culture Collection, and the strain number is CCTCC NO.M 2022622; The Bacillus thuringiensis is Bacillus thuringiensis HY-002, which is deposited in the China Center for Type Culture Collection (CCTCC), with a deposit number of CCTCC NO: M 20242390 and a deposit date of October 31, 2024.

4. A microecological preparation for improving livestock and poultry breeding environment according to claim 3, characterized in that: Bacillus subtilis 5×10 9 CFU / g, Bacillus licheniformis 5×10 9 CFU / g, Bacillus thuringiensis 5×10 8 CFU / g.

5. A microecological preparation for improving livestock and poultry breeding environment according to claim 3, characterized in that: Bacillus subtilis 5×10 9 CFU / g, Bacillus licheniformis 5×10 9 CFU / g, Bacillus thuringiensis 1×10 8 CFU / g.

6. A microecological preparation for improving livestock and poultry breeding environment according to claim 3, characterized in that: The Bacillus licheniformis is from Guangdong Microbiological Culture Collection Center, and the strain number is GDMCC1.

11.

7. A microecological preparation for improving livestock and poultry breeding environment according to claim 3, characterized in that: The carrier of the microecological preparation is maltodextrin.

8. According to claim 3, a microecological preparation for improving the livestock and poultry breeding environment is applied to the livestock and poultry breeding environment, and is directly diluted in water at a ratio of 1:1000 and then sprayed into the environment.

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