High-activity probiotic food for pets and preparation method of high-activity probiotic food

Abstract

The invention provides a high-activity probiotic food for pets, which is characterized by comprising the following components in parts by weight: 88-93 parts of animal meat source, 8-12 parts of boneless chicken powder, 8-12 parts of fish protein powder, 10-15 parts of potato powder, 3-6 parts of beer yeast powder, 3-6 parts of compound probiotics, 0.8-1.2 parts of metabiotics and 3-6 parts of semen plantaginis. Wherein the compound probiotics are prepared by mixing bacillus subtilis SCS-06, the preservation number of which is CCTCC (China Center for Type Culture Collection) NO: M20211498 and bacillus licheniformis XCL-09, the preservation number of which is CCTCC NO: M20211497. The prepared probiotic grain contains rich nutrient substances, probiotics and traditional Chinese medicine active ingredients, the content of active bacteria in the product is high, and the probiotic grain has a remarkable enhancing effect on digestion promoting ability and immunity of intestinal tracts and is effectively proliferated to continuously protect the intestinal tracts.

Description

Technical Field

[0001] The invention belongs to the technical field of pet food, and particularly relates to a highly active probiotic food for pets and a preparation method thereof. Background Art

[0002] At present, more and more families choose to keep cats as pets, and the corresponding pet food is becoming more and more abundant, with broad market prospects. However, due to the cat's own physiological structure, changes in the breeding environment, seasonal changes, changes in food, etc., the cat's body health problems may occur, resulting in soft stools, diarrhea, loss of appetite, and mental depression. At present, common probiotic cat food mostly solves the problems of cat's intestinal flora disorder and indigestion by adding probiotics. However, the current probiotic food cannot guarantee the activity of probiotics, and cannot guarantee that probiotics can effectively take root in the intestines.

[0003] Chinese patent CN113925114A discloses a cat food for regulating the stomach and improving digestion and absorption and its preparation method. After the cat eats, the feces formation is analyzed according to the Bristol feces classification method, which can improve the cat's defecation and reduce diarrhea. However, it does not involve whether it can improve the immunity of cats. Overall, the product function still needs to be improved. Summary of the invention

[0004] In view of this, the present invention provides a highly active probiotic food for pets and a preparation method thereof, wherein the probiotic food can not only maintain and improve the digestive ability, but also enable the probiotics to effectively take root in the intestines to improve the immune function of cats.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions: A highly active probiotic food for pets, comprising the following components in parts by weight: 88-93 parts of animal meat source, 8-12 parts of boneless chicken powder, 8-12 parts of fish protein powder, 10-15 parts of potato powder, 3-6 parts of brewer's yeast powder, 3-6 parts of compound probiotics, 0.8-1.2 parts of postbiotics, and 3-6 parts of psyllium seeds; The composite probiotics are prepared by mixing Bacillus subtilis SCS-06, with a preservation number of CCTCC NO: M20211498, and Bacillus licheniformis XCL-09, with a preservation number of CCTCC NO: M20211497.

[0006] In some specific embodiments, preferably, the following components are included by weight: 90 parts of animal meat source, 10 parts of boneless chicken meal, 10 parts of fish protein powder, 10 parts of potato flour, 5 parts of brewer's yeast powder, 5 parts of compound probiotics, 1 part of postbiotics, and 5 parts of psyllium; The mass ratio of Bacillus subtilis to Bacillus licheniformis in the composite probiotics is (3:7) to (7:3).

[0007] Furthermore, the postbiotics are prepared by mixing Lactobacillus reuteri, Bifidobacterium animalis and Lactobacillus plantarum.

[0008] In some specific embodiments, preferably, the mass ratio of Lactobacillus reuteri, Bifidobacterium animalis, and Lactobacillus plantarum is 1:1:1.

[0009] Furthermore, it is characterized in that the animal meat source includes fresh chicken and fresh duck meat.

[0010] In some specific embodiments, preferably, the mass ratio of fresh chicken meat to fresh duck meat is (7:3) to (3:7).

[0011] A method for preparing the above-mentioned highly active probiotic food for pets comprises the following steps: S1. Wash fresh chicken and duck meat, remove the fascia, and use a colloid mill to homogenize to obtain meat paste; S2, crushing and sieving psyllium and postbiotics, adding meat paste, bone-in chicken meal, fish protein powder, potato flour, brewer's yeast powder, and compound probiotics to homogenize to obtain a mixture; S3, adding water to the mixture, puffing, drying, cooling and packaging to obtain the highly active probiotic food for pets.

[0012] In some specific embodiments, preferably, the sieving in step S2 is sieving through a 80-120 mesh sieve, and the homogenization conditions are: a rotation speed of 8000-12000 rpm, and a time of 3 min.

[0013] In some specific embodiments, preferably, the amount of water added during the tempering in step S3 is 27-30wt% of the moisture content of the entire material, and the tempering temperature is 100-110°C; the steam pressure during the puffing process is 0.3MPa-0.8MPa, and the temperature is 80°C-140°C; the drying conditions are: the temperature is 100°C, and the moisture content is dried to 3%-7.5%.

[0014] Compared with the prior art, the present invention has the following beneficial effects: The number of viable probiotics in the probiotic food provided by the present invention reaches 5.0*10 10 CFU / kg or above, the probiotics are highly active and there is no inactivation in the product production process; feeding cats can significantly enhance their immunity, so that the levels of IgG (14.872~15.430g / L), IgM (1.639~1.722g / L), IgA (2.816~3.385g / L) and complement C3 (1.512~1.663g / L), and C4 (0.374~0.412g / L) in the body are significantly increased compared with conventional feeding.

[0015] This probiotic food uses a variety of fresh animal meat sources, is rich in nutrition, and contains live probiotics. Compared with postbiotics alone, it significantly enhances the digestion, protection and immune enhancement effects of the intestines. The addition of psyllium promotes digestion while effectively protecting the bacteria. Research has proven that it can regulate serum biochemical enzyme activity, enhance antioxidant capacity, and relieve inflammation of immune organs. DETAILED DESCRIPTION

[0016] The present invention is further described in detail below in conjunction with specific embodiments so that those skilled in the art can understand the present invention more clearly.

[0017] Sources and physical and chemical parameters of key test materials: Lactobacillus reuteri, Bifidobacterium animalis, and Lactobacillus plantarum in the postbiotics were purchased from Weikang Probiotics (Suzhou) Co., Ltd.; Brewer's yeast powder was purchased from Angel Yeast Co., Ltd.

[0018] The specific high-activity probiotic food for pets of the present invention is obtained as follows: First, 88 to 93 portions of animal meat are taken by weight, the fascia is removed after washing, and a colloid mill is used for homogenization to obtain meat paste; the animal meat source is composed of fresh chicken and fresh duck meat in a mass ratio of (7:3) to (3:7).

[0019] Then, 0.8-1.2 parts of postbiotics and 3-6 parts of psyllium seeds are taken by weight, placed in a grinder for grinding and then passed through an 80-120 mesh sieve, and the meat paste, 8-12 parts of bone-in chicken powder, 8-12 parts of fish protein powder, 10-15 parts of potato powder, 3-6 parts of brewer's yeast powder and 3-6 parts of compound probiotics are added to mix, and homogenized at a rotation speed of 8000-12000 rpm for 3 minutes to obtain a mixture.

[0020] Finally, the mixture obtained in the previous step is added with water until the moisture content of the whole material is 27-30wt%, and then tempered in a conditioner at 100-110°C; after the tempering, it is puffed at a steam pressure of 0.3MPa-0.8MPa and a temperature of 80°C-140°C; after the puffing, it is dried at 100°C to a moisture content of 3%-7.5%, and then packaged after cooling to obtain the highly active probiotic food for pets.

[0021] Example 1 This embodiment provides a highly active probiotic food for pets and a preparation method thereof, which are as follows: S1. Take 90 parts of animal meat source, 10 parts of boneless chicken powder, 10 parts of fish protein powder, 10 parts of potato powder, 5 parts of brewer's yeast powder, 5 parts of compound probiotics, 1 part of postbiotics, and 5 parts of psyllium by weight. Among them, the animal meat source is composed of fresh chicken and fresh duck meat in a mass ratio of 7:3; the compound probiotics are composed of Bacillus subtilis SCS-06 (preservation number CCTCC NO: M20211498) and Bacillus licheniformis XCL-09 (preservation number CCTCC NO: M20211497) in a mass ratio of 1:1; the postbiotics are composed of Lactobacillus reuteri, Bifidobacterium animalis, and Lactobacillus plantarum in a mass ratio of 1:1:1.

[0022] S2. After cleaning the animal meat source, remove the fascia part, and use a colloid mill to homogenize to obtain meat paste.

[0023] S3, taking postbiotics, putting psyllium seeds into a grinder for grinding and then passing through a 100-mesh sieve, adding the meat paste, bone and chicken meal, fish protein powder, potato powder, brewer's yeast powder, and compound probiotics in S2, mixing, homogenizing at a rotation speed of 10000 rpm for 3 minutes, to obtain a mixture.

[0024] S4, adding water to the mixture in S3 until the water content of the whole material is 28wt%, and then conditioning the mixture in a conditioner at 100°C; after conditioning, expanding the mixture at a steam pressure of 0.5MPa and a temperature of 120°C; after expanding, drying the mixture at 100°C until the water content is 5%, and then cooling and packaging the mixture to obtain the highly active probiotic food for pets.

[0025] Example 2 The present embodiment provides a highly active probiotic food for pets and a preparation method thereof, the steps and methods of which are basically the same as those of Example 1, except that: the specific materials in step S1 are 88 parts of animal meat source, 12 parts of boneless chicken meal, 8 parts of fish protein powder, 15 parts of potato flour, 3 parts of brewer's yeast powder, 6 parts of compound probiotics, 0.8 parts of postbiotics, and 6 parts of psyllium. Among them, the animal meat source is composed of fresh chicken and fresh duck meat in a mass ratio of 1:1; the compound probiotics are composed of Bacillus subtilis SCS-06 (preservation number CCTCCNO: M20211498) and Bacillus licheniformis XCL-09 (preservation number CCTCC NO: M20211497) in a mass ratio of 3:7; the postbiotics are composed of Lactobacillus reuteri, Bifidobacterium animalis, and Lactobacillus plantarum in a mass ratio of 1:1:1, Example 3 This embodiment provides a highly active probiotic food for pets and a preparation method thereof, the steps and methods of which are basically the same as those of Example 1, except that: the specific materials in step S1 are 93 parts of animal meat source, 8 parts of boneless chicken meal, 12 parts of fish protein powder, 10 parts of potato flour, 6 parts of brewer's yeast powder, 3 parts of compound probiotics, 1.2 parts of postbiotics, and 3 parts of psyllium. Among them, the animal meat source is composed of fresh chicken and fresh duck meat in a mass ratio of 7:3; the compound probiotics are composed of Bacillus subtilis SCS-06 (preservation number CCTCCNO: M20211498) and Bacillus licheniformis XCL-09 (preservation number CCTCC NO: M20211497) in a mass ratio of 7:3; the postbiotics are composed of Lactobacillus reuteri, Bifidobacterium animalis, and Lactobacillus plantarum in a mass ratio of 1:1:1.

[0026] Example 4 The present embodiment provides a highly active probiotic food for pets and a preparation method thereof, wherein the steps and methods are substantially the same as those of Embodiment 1, except that: in step S3, the mixture is sieved through an 80-mesh sieve at a rotation speed of 8000 rpm; in step S4, water is added to the mixture until the moisture content of the entire material is 30 wt %, and the mixture is tempered in a conditioner at 110° C.; after the conditioning is completed, the mixture is puffed at a steam pressure of 0.8 MPa and a temperature of 140° C.; after the puffing is completed, the mixture is dried at 100° C. until the moisture content is 7.5%, and the rest remain unchanged.

[0027] Example 5 The present embodiment provides a highly active probiotic food for pets and a preparation method thereof, wherein the steps and methods are substantially the same as those of Embodiment 1, except that: in step S3, the mixture is sieved through a 100-mesh sieve at a rotation speed of 12,000 rpm; in step S4, water is added to the mixture until the moisture content of the entire material is 27 wt %, and the mixture is tempered in a conditioner at 100° C.; after the conditioning is completed, the mixture is puffed at a steam pressure of 0.3 MPa and a temperature of 80° C.; after the puffing is completed, the mixture is dried at 100° C. until the moisture content is 3%, and the rest remain unchanged.

[0028] Comparative Example 1 This comparative example provides a highly active probiotic food for pets and a preparation method thereof, the steps and methods of which are basically the same as those of Example 1, except that: the animal meat source in step S1 is entirely chicken, and the rest remain unchanged.

[0029] Comparative Example 2 This comparative example provides a highly active probiotic food for pets and a preparation method thereof, wherein the steps and methods are substantially the same as those of Example 1, except that duck meat is used as the animal meat source in step S1, and the rest remain unchanged.

[0030] Comparative Example 3 This comparative example provides a highly active probiotic food for pets and a preparation method thereof. The steps and methods are basically the same as those in Example 1, except that: the composite probiotic in step S1 only uses Bacillus subtilis SCS-06, and the rest remain unchanged.

[0031] Comparative Example 4 This comparative example provides a highly active probiotic food for pets and a preparation method thereof. The steps and methods are basically the same as those in Example 1, except that: the Bacillus licheniformis XCL-09 in the composite probiotics in step S1 is replaced by conventional commercially available Bacillus licheniformis (purchased from Shandong Zhongke Jiayi Bioengineering Co., Ltd.), and the rest remain unchanged.

[0032] Comparative Example 5 This comparative example provides a highly active probiotic food for pets and a preparation method thereof, the steps and methods of which are basically the same as those in the embodiment, except that the composite probiotics are removed from the material in step S1, and the rest remain unchanged.

[0033] Comparative Example 6 This comparative example provides a highly active probiotic food for pets and a preparation method thereof, the steps and methods of which are basically the same as those in the embodiment, except that: in step S1, the postbiotics are removed from the material, and the rest remain unchanged.

[0034] Comparative Example 7 This comparative example provides a highly active probiotic food for pets and a preparation method thereof, wherein the steps and methods are substantially the same as those in the embodiment, except that the puffing temperature in step S3 is 160°C.

[0035] Comparative Example 8 This comparative example provides a highly active probiotic food for pets and a preparation method thereof, the steps and methods of which are basically the same as those in the embodiment, except that the moisture content after drying is 10%.

[0036] Furthermore, in order to understand the performance of the probiotic food prepared in the above embodiments and comparative examples, the following tests were also conducted: 1. Live bacteria rate test The specific test method is as follows: 1 Preparation: Weigh 10g of sample and place it in a 250mL conical flask filled with 89mL of sterile saline, 10-20 glass beads (2mm-3mm in diameter) and 1g of Tween 80. After sealing with a sterile breathable membrane, place it in a 50℃ water bath for 20min, then oscillate at 37℃ at 250r / min for 1h to prepare a 1:10 diluted sample solution.

[0037] 2 Dilution: 2.1 Use a 1mL sterile pipette or micropipette to draw 1mL of the 1:10 sample homogenate and slowly inject it into a sterile test tube containing 9mL of sterile saline along the tube wall. Use an oscillator to homogenize the sample or use a sterile pipette to repeatedly blow and mix it to make a 1:100 diluted sample homogenate.

[0038] 2.2 Use a 1 mL sterile pipette or micropipette tip to draw up 1 mL of the 1:100 sample solution. Follow the above steps to make 10-fold incremental dilutions of the sample solution. Use a 1 mL sterile pipette or pipette tip for each incremental dilution.

[0039] 3. Cultivation: According to the estimation of the bacterial content of the sample to be tested, select 2~3 continuous appropriate dilutions. Pipette 0.1mL of bacterial solution and inoculate it into the nutrient agar plate. Use a spreading stick to spread the inoculation solution on the agar surface carefully and quickly. The spreading stick cannot touch the edge of the plate. Inoculate 2 plates for each dilution. After spreading, let the plate stand for 10 minutes to allow the inoculum to be completely absorbed by the culture medium. Turn the plate over and place it in a 37℃±1℃ incubator for 24h~36h.

[0040] 4 Counting methods: 4.1 Select plates with characteristic colony counts between 20 CFU and 200 CFU and no spreading colony growth to count the total number of colonies. Plates with less than 20 CFU should record the specific number of colonies, and those with more than 200 CFU can be recorded as too many to count. The number of colonies for each dilution should be the average of two plates.

[0041] 4.2 If a plate has large flake-like colonies growing on it, it should not be used. Instead, the plate without flake-like colonies should be used as the colony count for that dilution. If the flake-like colonies are less than half of the plate, and the colonies in the remaining half are evenly distributed, then half of the plate can be calculated and x2 is used to represent the colony count of a plate.

[0042] 4.3 When there are chain-like growths without obvious boundaries between colonies on the plate, each single chain is counted as a colony. Table 1 is Table 1 Test results of viable bacteria rate of each probiotic food

[0043] It can be seen from Table 1 that the viable counts of probiotics in the products prepared in Examples 1-5 after testing all reached 5.0*10 10CFU / kg or more, the probiotics have a high degree of activity, and no inactivation occurs during the product production process, so that the probiotics can effectively reproduce in the pet's stomach and intestines to protect the intestines. Comparative Examples 1, 2, and 6 show that the change in meat source type and the lack of postbiotics do not affect the activity and content of probiotics. Comparative Examples 3-5 change the composition of composite probiotics, and it can be found that the use of specific strains can ensure the high content and high activity of the probiotics during the production process; Comparative Example 7 changes the puffing temperature, and temperature is a key factor affecting the activity of microorganisms, resulting in a decrease in the content and activity of probiotics; Comparative Example 8 changes the water content, which may affect the pH of the internal environment, etc., which also leads to a decrease in the content and activity of probiotics.

[0044] 2. Immunity Test The experimental cats in different groups were fed according to their body weight. On the 15th day, blood was collected from the medial saphenous vein of the forelimbs of 25 cats before feeding (blood volume 1-2 mL / time / cat). One portion was collected in an ordinary negative pressure blood collection tube and centrifuged at 5000 r / min for 10 min to separate the serum. The upper serum was transferred to each reagent cup for biochemical index detection, mainly for the detection of IgG, IgM, IgA and complement C3, C4 contents. During the feeding period, the cats were fed strictly according to the experimental requirements, and the situation was carefully observed and recorded. The results are shown in Table 2.

[0045] Table 2 Test results of pets' immune ability on the 15th day after consuming various probiotic foods

[0046] It can be seen from Table 2 that after testing the products of Examples 1-5, it can be seen that the various immune indicators are significantly higher than those of the group fed with conventional pet food, indicating that feeding probiotic food effectively improves the immunity of the experimental subjects; Comparative Examples 1-6 were found to have lower immune indicators than the comparative examples after testing, which changed the composition of the materials, resulting in changes in the activity of the strains and the nutritional composition of the pet food; similarly, due to changes in process parameters in Comparative Examples 7-8, the probiotic activity and nutritional composition did not meet expectations, resulting in a decline in various immune indicators.

[0047] Other raw materials or structures not specifically described in the present invention already exist in the prior art and can be directly purchased from the market.

[0048] The above are only preferred embodiments of the present invention and are not intended to limit the protection scope of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A highly active probiotic food for pets, characterized in that: The invention comprises the following components by weight: 88-93 parts of animal meat source, 8-12 parts of boneless chicken powder, 8-12 parts of fish protein powder, 10-15 parts of potato powder, 3-6 parts of brewer's yeast powder, 3-6 parts of compound probiotics, 0.8-1.2 parts of postbiotics, and 3-6 parts of psyllium seeds; The composite probiotics are prepared by mixing Bacillus subtilis SCS-06, with a preservation number of CCTCC NO: M20211498, and Bacillus licheniformis XCL-09, with a preservation number of CCTCC NO: M20211497.

2. The highly active probiotic food for pets according to claim 1, characterized in that: The invention comprises the following components by weight: 90 parts of animal meat source, 10 parts of boneless chicken powder, 10 parts of fish protein powder, 10 parts of potato powder, 5 parts of brewer's yeast powder, 5 parts of compound probiotics, 1 part of postbiotics, and 5 parts of psyllium; The mass ratio of Bacillus subtilis to Bacillus licheniformis in the composite probiotics is (3:7) to (7:3).

3. The highly active probiotic food for pets according to claim 1 or 2, characterized in that: The postbiotics are prepared by mixing Lactobacillus reuteri, Bifidobacterium animalis and Lactobacillus plantarum.

4. The highly active probiotic food for pets according to claim 3, characterized in that: The mass ratio of the Lactobacillus reuteri, Bifidobacterium animalis and Lactobacillus plantarum is 1:1:

1.

5. The highly active probiotic food for pets according to any one of claims 1 to 4, characterized in that: The animal meat sources include fresh chicken and fresh duck.

6. The highly active probiotic food for pets according to claim 5, characterized in that: The mass ratio of fresh chicken meat to fresh duck meat is (7:3) to (3:7).

7. A method for preparing the highly active probiotic food for pets according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1. Wash fresh chicken and duck meat, remove the fascia, and use a colloid mill to homogenize to obtain meat paste; S2, crushing and sieving psyllium and postbiotics, adding meat paste, bone-in chicken meal, fish protein powder, potato flour, brewer's yeast powder, and compound probiotics to homogenize to obtain a mixture; S3, adding water to the mixture to adjust the quality, puffing, drying, cooling and packaging to obtain the highly active probiotic food for pets.

8. The method according to claim 7, characterized in that The sieving in step S2 is through a 80-120 mesh sieve, and the homogenization conditions are: a rotation speed of 8000-12000 rpm and a time of 3 min.

9. The method according to claim 7, characterized in that: The amount of water added during the conditioning in step S3 is 27-30wt% of the moisture content of the entire material, and the conditioning temperature is 100-110°C; the steam pressure during the puffing process is 0.3MPa-0.8MPa, and the temperature is 80°C-140°C; the drying conditions are: the temperature is 100°C, and the moisture content is dried to 3%-7.5%.

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