Traditional Chinese medicine preparation for treating osteoporosis and preparation method thereof

Abstract

The invention discloses a traditional Chinese medicine preparation for treating osteoporosis and a preparation method, and relates to the technical field of traditional Chinese medicine preparations, and the traditional Chinese medicine preparation is prepared from the following components: sargentgloryvine stem, fructus kochiae, caulis spatholobi, liquorice, poria cocos, semen cuscutae, salvia chinensis, a salvia miltiorrhiza compound, radix paeoniae alba, codonopsis pilosula, semen armeniacae amarae, cinnamon and corn starch. The prepared traditional Chinese medicine preparation is mainly used for treating osteoporosis symptoms, and the effects of promoting osteoblast development, improving bone mineral density and relieving the osteoporosis symptoms are achieved by means of cooperation of all the components, cooperation of multiple active ingredients and regulation and control of expression of some signal channels.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine preparations, in particular to a traditional Chinese medicine preparation for treating osteoporosis. Background Art

[0002] Osteoporosis not only greatly increases the risk of fractures in elderly patients, but also seriously reduces their ability to live and motor function. Osteoporosis is a systemic bone disease characterized by decreased bone density, low bone mass, and bone microstructure destruction, which can easily lead to fractures. With the aging of the population, osteoporosis, as a common geriatric disease, has seriously affected the quality of life and physical and mental health of the elderly.

[0003] Osteoporosis is a systemic bone disease caused by a variety of reasons. Its main characteristics are the decrease in bone density and bone quality, as well as the damage of bone microstructure. These changes make bones fragile, thereby increasing the risk of fractures. Symptoms of osteoporosis include:

[0004] 1. Pain: Osteoporosis patients often experience back pain or body bone pain, especially when turning over, sitting up, and after walking for a long time. The pain worsens at night or during weight-bearing activities.

[0005] 2. Fatigue: Patients easily feel tired, fatigue increases after simple labor, and the weight-bearing capacity is significantly reduced.

[0006] 3. Spinal deformity: Patients with severe osteoporosis may experience spinal deformities such as shortened height and hunchback. Multiple thoracic vertebrae compression fractures can lead to chest deformity and even affect cardiopulmonary function.

[0007] 4. Fractures: Fractures caused by slight external force and simple movement are called brittle fractures. They are common complications of osteoporosis and are prone to occur in the thoracic and lumbar spine, hips, distal forearms and other parts.

[0008] For patients with osteoporosis, most are treated with medication, but chemical drugs have potential risks and side effects, and have varying degrees of negative effects on the bodies of older people.

[0009] Based on this, a Chinese medicine preparation for treating osteoporosis is provided to solve the existing technical problems. Summary of the invention

[0010] The purpose of the present invention is to provide a Chinese medicine preparation for treating osteoporosis and a preparation method thereof, so as to solve the deficiencies in the prior art.

[0011] The technical solution adopted by the present invention is as follows:

[0012] A traditional Chinese medicine preparation for treating osteoporosis comprises the following ingredients by weight: 15-18 parts of red cauliflower, 3-5 parts of Kochia scoparia, 1-2 parts of Millettia reticulata, 10-12 parts of liquorice, 1-3 parts of Poria, 2-4 parts of Cuscuta australis, 1-1.8 parts of Lithospermum officinale, 3-5 parts of salvia miltiorrhiza complex, 1-3 parts of white peony root, 2-5 parts of Codonopsis pilosula, 4-6 parts of bitter almond, 2-3 parts of cinnamon bark and 1-2 parts of corn starch.

[0013] As a further technical solution, the preparation method of the Danshen complex is:

[0014] (1) First, pre-treat the salvia miltiorrhiza with a sodium chloride solution for 3-4 hours, then wash and drain to obtain pre-treated salvia miltiorrhiza;

[0015] (2) mixing the pretreated salvia miltiorrhiza with water and beating the mixture to obtain salvia miltiorrhiza slurry;

[0016] (3) first peeling the fresh pinellia to obtain peeled pinellia;

[0017] The peeled pinellia tuber is irradiated with far infrared radiation for 2-5 minutes to obtain the pinellia tuber after enzyme inhibition;

[0018] The irradiated pinellia tuber is subjected to microwave drying to obtain dried pinellia tuber;

[0019] Grinding the dried pinellia tuber to obtain pinellia tuber powder;

[0020] (4) adding the pinellia powder, salvia miltiorrhiza slurry, and water in sequence, and then stirring and mixing them evenly to obtain a mixed slurry;

[0021] (5) decocting the mixed slurry for 2 hours to obtain a decocted mixed solution;

[0022] (6) filtering the decoction mixture, concentrating the obtained filtrate, and obtaining the Danshen complex.

[0023] As a further technical solution, the method for pre-treating the salvia miltiorrhiza in step (1) is:

[0024] First, add Danshen to the sodium chloride solution, adjust the temperature to 40°C, let it stand, then filter and drain;

[0025] Wherein, the mass fraction of the sodium chloride solution is 15%;

[0026] The mixing ratio of Danshen and sodium chloride solution is 5g:120mL.

[0027] As a further technical solution, the mixing ratio of the pretreated Danshen and water in step (2) is 10 g: 100 mL;

[0028] Among them, the beating speed is 5000r / min.

[0029] As a further technical solution: in step (3), the irradiation intensity of far-infrared irradiation is 1100-1300 W / m 2 .

[0030] As a further technical solution, the mixing ratio of the pinellia powder, salvia miltiorrhiza slurry and water in step (4) is: 50-55g: 20-23g: 350-380mL.

[0031] As a further technical solution, in step (5), the decocting is firstly boiled over high heat, then decocted over low heat.

[0032] As a further technical solution: in step (6), the filtrate is concentrated to 1% of the original volume.

[0033] A method for preparing a traditional Chinese medicine preparation for treating osteoporosis comprises the following steps:

[0034] S1 Preparation:

[0035] Weigh the following components according to the mass ratio: red caulis rubra, Kochia scoparia, Millettia spatholobi, licorice, Poria, Cuscuta australis, Lithospermum officinale, salvia miltiorrhiza complex, white peony root, Codonopsis pilosula, bitter almond, cinnamon bark, corn starch;

[0036] S2 material division:

[0037] Divide each component into three groups:

[0038] The first group includes: red vine, liquorice, Poria, dodder seed, bitter almond, cinnamon bark;

[0039] The second group includes: Kochia scoparia, Millettia reticulata, Lithospermum officinale, White Peony Root, Codonopsis pilosula;

[0040] The third group is: Danshen complex;

[0041] S3 grouping extraction:

[0042] For the first group, the raw materials of the first group were mixed in sequence, and then water of 5 times the total mass of the mixture was added, boiled over high heat, and then simmered over low heat for 2 hours, filtered, and concentrated to 5% of the original volume to obtain the first extract;

[0043] For the second group, the second group of raw materials were mixed in sequence, and then an ethanol solution of 5 times the total mass of the mixture was added, and the mixture was soaked at room temperature for 10 hours, and then stirred for 2 hours, filtered to obtain a filtrate, and the operation was repeated twice on the filter residue, and the filtrate was combined, and the filtrate was decompressed at a temperature of 30°C to recover ethanol, and then concentrated to 5% of the original volume to obtain a second extract;

[0044] S4: mixing the first extract, the second extract, and the Danshen complex together, adding corn starch, mixing, granulating, and drying to obtain a traditional Chinese medicine preparation;

[0045] Dry at 50°C for 18 hours.

[0046] Beneficial effects:

[0047] The traditional Chinese medicine preparation prepared by the present invention is mainly used to treat osteoporosis symptoms. The present invention promotes osteoblast development, improves bone density, and alleviates osteoporosis symptoms through the coordinated action of various components, the coordination of multiple active ingredients, and the regulation of the expression of some signal pathways.

[0048] The present invention introduces the Danshen complex, and its active ingredients have the function of regulating endocrine-related hormones, thereby inhibiting the differentiation of osteoclasts and preventing the reduction of bone density. In addition, it can regulate reactive oxygen and greatly reduce the inflammatory effect on osteoblasts, thus greatly improving the symptoms of osteoporosis.

[0049] Through the coordinated effect of Danshen complex with Poria cocos and licorice, it can mainly play a role in significantly increasing the body's renal tubular reabsorption of calcium and phosphorus, promoting the absorption of calcium and phosphorus in the intestine, greatly reducing the loss of calcium and phosphorus, and thus significantly increasing blood calcium and phosphorus levels. In this way, it can promote the growth of bone cells, increase bone density, and reduce osteoporosis symptoms.

[0050] The Chinese medicine preparation prepared by the present invention has the function of regulating the synthesis of prostaglandin collagen. Through its regulatory function, it can not only inhibit its excessive increase, but also prevent its decrease, so that it can continuously maintain a dynamic equilibrium state in the body. This dynamic equilibrium state plays an important role in the health and recovery of bones and can shorten the recovery time of osteoporosis.

[0051] Through the coordinated action of the complex of Kochia scoparia, Millettia reticulata, Lithospermum officinale, White Peony Root, Codonopsis pilosula, and Salvia miltiorrhiza, the sensitivity of the thyroid gland to estrogen can be increased, and the secretion of calcitonin by thyroid C cells can be enhanced. Since calcitonin is an important hormone, it mainly plays a role in inhibiting the activity of osteoclasts, thereby reducing bone absorption, and then improving and enhancing bone stability and strength.

[0052] The Chinese medicine preparation of the present invention can promote the proliferation and differentiation of osteoblasts. Since osteoblasts are the cell type responsible for bone formation, their activity is directly related to the speed and quality of new bone formation. By increasing their activity, the formation metabolism can be improved, bone density can be increased, and osteoporosis can be improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0053] Figure 1Statistical graphs of femoral neck bone density before and after treatment in each group of the embodiment and comparative example. DETAILED DESCRIPTION

[0054] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0055] Example 1

[0056] A method for preparing a traditional Chinese medicine preparation for treating osteoporosis comprises the following steps:

[0057] S1 Preparation:

[0058] Weigh each component according to the mass ratio: 15 parts of red cauline, 3 parts of Kochia scoparia, 1 part of Millettia reticulata, 10 parts of Licorice, 1 part of Poria, 2 parts of Cuscuta australis, 1 part of Lithospermum officinale, 3 parts of Salvia miltiorrhiza complex, 1 part of White Peony Root, 2 parts of Codonopsis pilosula, 4 parts of Bitter Apricot, 2 parts of Cinnamon Bark, and 1 part of Corn Starch.

[0059] The preparation method of the Danshen complex is as follows:

[0060] (1) First, the salvia miltiorrhiza is pretreated with a sodium chloride solution for 3 hours, and then washed and drained to obtain a pretreated salvia miltiorrhiza; the pretreated salvia miltiorrhiza treatment method is:

[0061] First, add Danshen to the sodium chloride solution, adjust the temperature to 40°C, let it stand, then filter and drain;

[0062] Wherein, the mass fraction of the sodium chloride solution is 15%;

[0063] The mixing ratio of Danshen and sodium chloride solution is 5g:120mL;

[0064] (2) mixing the pretreated salvia miltiorrhiza and water to obtain salvia miltiorrhiza slurry; the mixing ratio of the pretreated salvia miltiorrhiza and water is 10 g: 100 mL;

[0065] Among them, the beating speed is 5000r / min.

[0066] (3) first peeling the fresh pinellia to obtain peeled pinellia;

[0067] The peeled pinellia tuber is irradiated with far-infrared radiation for 2 minutes to obtain the pinellia tuber after enzyme inhibition;

[0068] The irradiated pinellia tuber is subjected to microwave drying to obtain dried pinellia tuber;

[0069] The dried pinellia tuber is crushed to obtain pinellia tuber powder; the irradiation intensity of the far infrared irradiation is 1100W / m 2 ;

[0070] (4) adding pinellia powder, salvia miltiorrhiza slurry and water together in sequence, and then stirring and mixing to obtain a mixed slurry; the mixing ratio of pinellia powder, salvia miltiorrhiza slurry and water is: 50g:20g:350mL;

[0071] (5) decocting the mixed slurry for 2 hours to obtain a decocted mixed solution;

[0072] To cook, first boil over high heat, then continue to cook over low heat.

[0073] (6) filtering the decoction mixture, concentrating the obtained filtrate to obtain the Danshen complex; and concentrating the filtrate to 1% of the original volume.

[0074] S2 material division:

[0075] Divide each component into three groups:

[0076] The first group includes: red vine, liquorice, Poria, dodder seed, bitter almond, cinnamon bark;

[0077] The second group includes: Kochia scoparia, Millettia reticulata, Lithospermum officinale, White Peony Root, Codonopsis pilosula;

[0078] The third group is: Danshen complex;

[0079] S3 grouping extraction:

[0080] For the first group, the raw materials of the first group were mixed in sequence, and then water of 5 times the total mass of the mixture was added, boiled over high heat, and then simmered over low heat for 2 hours, filtered, and concentrated to 5% of the original volume to obtain the first extract;

[0081] For the second group, the second group of raw materials were mixed in sequence, and then an ethanol solution of 5 times the total mass of the mixture was added, and the mixture was soaked at room temperature for 10 hours, and then stirred for 2 hours, filtered to obtain a filtrate, and the operation was repeated twice on the filter residue, and the filtrate was combined, and the filtrate was decompressed at a temperature of 30°C to recover ethanol, and then concentrated to 5% of the original volume to obtain a second extract;

[0082] S4: mixing the first extract, the second extract, and the Danshen complex together, adding corn starch, mixing, granulating, and drying to obtain a traditional Chinese medicine preparation;

[0083] Dry at 50°C for 18 hours.

[0084] Example 2

[0085] A method for preparing a traditional Chinese medicine preparation for treating osteoporosis comprises the following steps:

[0086] S1 Preparation:

[0087] Weigh each component according to the mass ratio: 16 parts of red cauline, 4 parts of Kochia scoparia, 1.5 parts of Millettia spatholobi, 11 parts of Licorice, 2 parts of Poria, 2.5 parts of Cuscuta australis, 1.2 parts of Lithospermum officinale, 3.5 parts of Salvia miltiorrhiza complex, 2 parts of White Peony Root, 3 parts of Codonopsis pilosula, 5 parts of Bitter Apricot, 2.5 parts of Cinnamon Bark, and 1.2 parts of Corn Starch.

[0088] The preparation method of the Danshen complex is as follows:

[0089] (1) First, the salvia miltiorrhiza was pretreated with a sodium chloride solution for 3.5 hours, and then washed and drained to obtain a pretreated salvia miltiorrhiza; the pretreated salvia miltiorrhiza treatment method was as follows:

[0090] First, add Danshen to the sodium chloride solution, adjust the temperature to 40°C, let it stand, then filter and drain;

[0091] Wherein, the mass fraction of the sodium chloride solution is 15%;

[0092] The mixing ratio of Danshen and sodium chloride solution is 5g:120mL;

[0093] (2) mixing the pretreated salvia miltiorrhiza and water to obtain salvia miltiorrhiza slurry; the mixing ratio of the pretreated salvia miltiorrhiza and water is 10 g: 100 mL;

[0094] Among them, the beating speed is 5000r / min.

[0095] (3) first peeling the fresh pinellia to obtain peeled pinellia;

[0096] The peeled pinellia tuber is irradiated with far-infrared radiation for 3 minutes to obtain the pinellia tuber after enzyme inhibition;

[0097] The irradiated pinellia tuber is subjected to microwave drying to obtain dried pinellia tuber;

[0098] The dried pinellia tuber is crushed to obtain pinellia tuber powder; the irradiation intensity of the far infrared irradiation is 1100-1300W / m 2 ;

[0099] (4) adding pinellia powder, salvia miltiorrhiza slurry, and water together in sequence, and then stirring and mixing to obtain a mixed slurry; the mixing ratio of pinellia powder, salvia miltiorrhiza slurry, and water is: 52g:21g:360mL;

[0100] (5) decocting the mixed slurry for 2 hours to obtain a decocted mixed solution;

[0101] To cook, first boil over high heat, then continue to cook over low heat.

[0102] (6) filtering the decoction mixture, concentrating the obtained filtrate to obtain the Danshen complex; and concentrating the filtrate to 1% of the original volume.

[0103] S2 material division:

[0104] Divide each component into three groups:

[0105] The first group includes: red vine, liquorice, Poria, dodder seed, bitter almond, cinnamon bark;

[0106] The second group includes: Kochia scoparia, Millettia reticulata, Lithospermum officinale, White Peony Root, Codonopsis pilosula;

[0107] The third group is: Danshen complex;

[0108] S3 grouping extraction:

[0109] For the first group, the raw materials of the first group were mixed in sequence, and then water of 5 times the total mass of the mixture was added, boiled over high heat, and then simmered over low heat for 2 hours, filtered, and concentrated to 5% of the original volume to obtain the first extract;

[0110] For the second group, the second group of raw materials were mixed in sequence, and then an ethanol solution of 5 times the total mass of the mixture was added, and the mixture was soaked at room temperature for 10 hours, and then stirred for 2 hours, filtered to obtain a filtrate, and the operation was repeated twice on the filter residue, and the filtrate was combined, and the filtrate was decompressed at a temperature of 30°C to recover ethanol, and then concentrated to 5% of the original volume to obtain a second extract;

[0111] S4: mixing the first extract, the second extract, and the Danshen complex together, adding corn starch, mixing, granulating, and drying to obtain a traditional Chinese medicine preparation;

[0112] Dry at 50°C for 18 hours.

[0113] Example 3

[0114] A method for preparing a traditional Chinese medicine preparation for treating osteoporosis comprises the following steps:

[0115] S1 Preparation:

[0116] Weigh each component according to the mass ratio: 17 parts of red cauline, 4 parts of Kochia scoparia, 1.5 parts of Millettia spatholobi, 11 parts of Licorice, 2 parts of Poria, 3 parts of Cuscuta, 1.5 parts of Lithospermum officinale, 4 parts of Salvia miltiorrhiza complex, 2 parts of White Peony Root, 3 parts of Codonopsis pilosula, 5 parts of Bitter Apricot, 2.5 parts of Cinnamon Bark, and 1.6 parts of Corn Starch.

[0117] The preparation method of the Danshen complex is as follows:

[0118] (1) First, the salvia miltiorrhiza was pretreated with a sodium chloride solution for 3.5 hours, and then washed and drained to obtain a pretreated salvia miltiorrhiza; the pretreated salvia miltiorrhiza treatment method was as follows:

[0119] First, add Danshen to the sodium chloride solution, adjust the temperature to 40°C, let it stand, then filter and drain;

[0120] Wherein, the mass fraction of the sodium chloride solution is 15%;

[0121] The mixing ratio of Danshen and sodium chloride solution is 5g:120mL;

[0122] (2) mixing the pretreated salvia miltiorrhiza and water to obtain salvia miltiorrhiza slurry; the mixing ratio of the pretreated salvia miltiorrhiza and water is 10 g: 100 mL;

[0123] Among them, the beating speed is 5000r / min.

[0124] (3) first peeling the fresh pinellia to obtain peeled pinellia;

[0125] The peeled pinellia tuber is irradiated with far-infrared radiation for 3 minutes to obtain the pinellia tuber after enzyme inhibition;

[0126] The irradiated pinellia tuber is subjected to microwave drying to obtain dried pinellia tuber;

[0127] The dried pinellia tuber is crushed to obtain pinellia tuber powder; the irradiation intensity of the far infrared irradiation is 1200W / m 2 ;

[0128] (4) adding pinellia powder, salvia miltiorrhiza slurry and water in sequence, and then stirring and mixing to obtain a mixed slurry; the mixing ratio of pinellia powder, salvia miltiorrhiza slurry and water is: 54g:22g:360mL;

[0129] (5) decocting the mixed slurry for 2 hours to obtain a decocted mixed solution;

[0130] To cook, first boil over high heat, then continue to cook over low heat.

[0131] (6) filtering the decoction mixture, concentrating the obtained filtrate to obtain the Danshen complex; and concentrating the filtrate to 1% of the original volume.

[0132] S2 material division:

[0133] Divide each component into three groups:

[0134] The first group includes: red vine, liquorice, Poria, dodder seed, bitter almond, cinnamon bark;

[0135] The second group includes: Kochia scoparia, Millettia reticulata, Lithospermum officinale, White Peony Root, Codonopsis pilosula;

[0136] The third group is: Danshen complex;

[0137] S3 grouping extraction:

[0138] For the first group, the raw materials of the first group were mixed in sequence, and then water of 5 times the total mass of the mixture was added, boiled over high heat, and then simmered over low heat for 2 hours, filtered, and concentrated to 5% of the original volume to obtain the first extract;

[0139] For the second group, the second group of raw materials were mixed in sequence, and then an ethanol solution of 5 times the total mass of the mixture was added, and the mixture was soaked at room temperature for 10 hours, and then stirred for 2 hours, filtered to obtain a filtrate, and the operation was repeated twice on the filter residue, and the filtrate was combined, and the filtrate was decompressed at a temperature of 30°C to recover ethanol, and then concentrated to 5% of the original volume to obtain a second extract;

[0140] S4: mixing the first extract, the second extract, and the Danshen complex together, adding corn starch, mixing, granulating, and drying to obtain a traditional Chinese medicine preparation;

[0141] Dry at 50°C for 18 hours.

[0142] Example 4

[0143] A method for preparing a traditional Chinese medicine preparation for treating osteoporosis comprises the following steps:

[0144] S1 Preparation:

[0145] Weigh each component according to the mass ratio: 18 parts of red cauline, 3 parts of Kochia scoparia, 2 parts of Millettia spatholobi, 11 parts of Licorice, 2 parts of Poria, 3 parts of Cuscuta, 1.5 parts of Lithospermum officinale, 4 parts of Salvia miltiorrhiza complex, 2 parts of White Peony Root, 3 parts of Codonopsis pilosula, 6 parts of Bitter Apricot, 2 parts of Cinnamon Bark, and 1.5 parts of Corn Starch.

[0146] The preparation method of the Danshen complex is as follows:

[0147] (1) First, the salvia miltiorrhiza is pretreated with a sodium chloride solution for 4 hours, and then washed and drained to obtain a pretreated salvia miltiorrhiza; the pretreated salvia miltiorrhiza treatment method is:

[0148] First, add Danshen to the sodium chloride solution, adjust the temperature to 40°C, let it stand, then filter and drain;

[0149] Wherein, the mass fraction of the sodium chloride solution is 15%;

[0150] The mixing ratio of Danshen and sodium chloride solution is 5g:120mL;

[0151] (2) mixing the pretreated salvia miltiorrhiza and water to obtain salvia miltiorrhiza slurry; the mixing ratio of the pretreated salvia miltiorrhiza and water is 10 g: 100 mL;

[0152] Among them, the beating speed is 5000r / min.

[0153] (3) first peeling the fresh pinellia to obtain peeled pinellia;

[0154] The peeled pinellia tuber is irradiated with far-infrared radiation for 3 minutes to obtain the pinellia tuber after enzyme inhibition;

[0155] The irradiated pinellia tuber is subjected to microwave drying to obtain dried pinellia tuber;

[0156] The dried pinellia tuber is crushed to obtain pinellia tuber powder; the irradiation intensity of the far infrared irradiation is 1200W / m 2 ;

[0157] (4) adding pinellia powder, salvia miltiorrhiza slurry, and water together in sequence, and then stirring and mixing to obtain a mixed slurry; the mixing ratio of pinellia powder, salvia miltiorrhiza slurry, and water is: 53g:22g:360mL;

[0158] (5) decocting the mixed slurry for 2 hours to obtain a decocted mixed solution;

[0159] To cook, first boil over high heat, then continue to cook over low heat.

[0160] (6) filtering the decoction mixture, concentrating the filtrate to obtain a Danshen complex; and concentrating the filtrate to 1% of the original volume.

[0161] S2 material division:

[0162] Divide each component into three groups:

[0163] The first group includes: red vine, liquorice, Poria, dodder seed, bitter almond, cinnamon bark;

[0164] The second group includes: Kochia scoparia, Millettia reticulata, Lithospermum officinale, White Peony Root, Codonopsis pilosula;

[0165] The third group is: Danshen complex;

[0166] S3 grouping extraction:

[0167] For the first group, the raw materials of the first group were mixed in sequence, and then water of 5 times the total mass of the mixture was added, boiled over high heat, and then simmered over low heat for 2 hours, filtered, and concentrated to 5% of the original volume to obtain the first extract;

[0168] For the second group, the second group of raw materials were mixed in sequence, and then an ethanol solution of 5 times the total mass of the mixture was added, and the mixture was soaked at room temperature for 10 hours, and then stirred for 2 hours, filtered to obtain a filtrate, and the operation was repeated twice on the filter residue, and the filtrate was combined, and the filtrate was decompressed at a temperature of 30°C to recover ethanol, and then concentrated to 5% of the original volume to obtain a second extract;

[0169] S4: mixing the first extract, the second extract, and the Danshen complex together, adding corn starch, mixing, granulating, and drying to obtain a traditional Chinese medicine preparation;

[0170] Dry at 50°C for 18 hours.

[0171] Example 5

[0172] A method for preparing a traditional Chinese medicine preparation for treating osteoporosis comprises the following steps:

[0173] S1 Preparation:

[0174] Weigh each component according to the mass ratio: 18 parts of red cauline, 5 parts of Kochia scoparia, 2 parts of Millettia spatholobi, 12 parts of Licorice, 3 parts of Poria, 4 parts of Cuscuta, 1.8 parts of Lithospermum officinale, 5 parts of Salvia miltiorrhiza complex, 3 parts of White Peony Root, 5 parts of Codonopsis pilosula, 6 parts of Bitter Apricot, 3 parts of Cinnamon Bark, and 2 parts of Corn Starch.

[0175] The preparation method of the Danshen complex is as follows:

[0176] (1) First, the salvia miltiorrhiza is pretreated with a sodium chloride solution for 4 hours, and then washed and drained to obtain a pretreated salvia miltiorrhiza; the pretreated salvia miltiorrhiza treatment method is:

[0177] First, add Danshen to the sodium chloride solution, adjust the temperature to 40°C, let it stand, then filter and drain;

[0178] Wherein, the mass fraction of the sodium chloride solution is 15%;

[0179] The mixing ratio of Danshen and sodium chloride solution is 5g:120mL;

[0180] (2) mixing the pretreated salvia miltiorrhiza and water to obtain salvia miltiorrhiza slurry; the mixing ratio of the pretreated salvia miltiorrhiza and water is 10 g: 100 mL;

[0181] Among them, the beating speed is 5000r / min.

[0182] (3) first peeling the fresh pinellia to obtain peeled pinellia;

[0183] The peeled pinellia tuber is irradiated with far-infrared radiation for 3 minutes to obtain the pinellia tuber after enzyme inhibition;

[0184] The irradiated pinellia tuber is subjected to microwave drying to obtain dried pinellia tuber;

[0185] The dried pinellia tuber is crushed to obtain pinellia tuber powder; the irradiation intensity of the far infrared irradiation is 1200W / m 2 ;

[0186] (4) adding pinellia powder, salvia miltiorrhiza slurry, and water together in sequence, and then stirring and mixing to obtain a mixed slurry; the mixing ratio of pinellia powder, salvia miltiorrhiza slurry, and water is: 55g:23g:380mL;

[0187] (5) decocting the mixed slurry for 2 hours to obtain a decocted mixed solution;

[0188] To cook, first boil over high heat, then continue to cook over low heat.

[0189] (6) filtering the decoction mixture, concentrating the obtained filtrate to obtain the Danshen complex; and concentrating the filtrate to 1% of the original volume.

[0190] S2 material division:

[0191] Divide each component into three groups:

[0192] The first group includes: red vine, liquorice, Poria, dodder seed, bitter almond, cinnamon bark;

[0193] The second group includes: Kochia scoparia, Millettia reticulata, Lithospermum officinale, White Peony Root, Codonopsis pilosula;

[0194] The third group is: Danshen complex;

[0195] S3 grouping extraction:

[0196] For the first group, the raw materials of the first group were mixed in sequence, and then water of 5 times the total mass of the mixture was added, boiled over high heat, and then simmered over low heat for 2 hours, filtered, and concentrated to 5% of the original volume to obtain the first extract;

[0197] For the second group, the second group of raw materials were mixed in sequence, and then an ethanol solution of 5 times the total mass of the mixture was added, and the mixture was soaked at room temperature for 10 hours, and then stirred for 2 hours, filtered to obtain a filtrate, and the operation was repeated twice on the filter residue, and the filtrate was combined, and the filtrate was decompressed at a temperature of 30°C to recover ethanol, and then concentrated to 5% of the original volume to obtain a second extract;

[0198] S4: mixing the first extract, the second extract, and the Danshen complex together, adding corn starch, mixing, granulating, and drying to obtain a traditional Chinese medicine preparation;

[0199] Dry at 50°C for 18 hours.

[0200] Comparative Example 1:

[0201] Based on Example 1, no Danshen complex is added, and the rest of the technical solutions remain unchanged.

[0202] Comparative Example 2:

[0203] On the basis of Example 1, the salvia miltiorrhiza complex is replaced with salvia miltiorrhiza powder, and the rest of the technical solutions remain unchanged.

[0204] test:

[0205] Total flavonoid content: The total flavonoid content in each group of the embodiment was determined by ultraviolet light using the sodium nitrite-aluminum nitrate-sodium hydroxide method:

[0206] Table 1

[0207] Total flavonoids mg / g Example 1 1.325 Example 2 1.286 Example 3 1.314 Example 4 1.328 Example 5 1.316

[0208] It can be seen from Table 1 that the Chinese medicine preparation prepared by the present invention has a higher flavonoid content.

[0209] The samples of the embodiment and the comparative example were tested for drug safety, and all of them were safe and non-toxic.

[0210] 70 elderly osteoporosis patients aged 55-65 years, half male and half female;

[0211] Exclude patients with other diseases that may affect osteoporosis.

[0212] Randomly divided into 7 groups:

[0213] The samples of the embodiment and the comparative example were respectively diluted with water at a mass ratio of 1:20 and taken twice a day for 5 consecutive days as a course of treatment, and 8 courses of treatment were performed continuously;

[0214] Efficacy criteria:

[0215] a Significant effect: The pain basically disappears, the symptoms of osteoporosis are greatly alleviated, and the bone density is significantly increased after testing;

[0216] bEffective: The pain is significantly relieved, the symptoms of osteoporosis are significantly improved, and the bone density is not reduced after testing;

[0217] cIneffective: The pain persists, the symptoms of osteoporosis do not improve, and the bone density is tested to be decreased.

[0218] Table 2

[0219] Significantly effective / case Valid / Example Invalid / Example Effectiveness% Example 1 6 3 1 90 Example 2 9 1 0 100 Example 3 6 4 0 100 Example 4 8 2 0 100 Example 5 7 3 0 100 Comparative Example 1 1 6 3 70 Comparative Example 2 4 4 2 80

[0220] It can be seen from Table 2 that the Chinese medicine preparation prepared by the present invention can effectively improve osteoporosis.

[0221] Before and after treatment, X-ray bone densitometer was used to detect the average bone density of lumbar vertebrae L1-4 and femoral neck of the embodiment and comparative example groups before and after treatment: see Tables 3 and 4:

[0222] Table 3 Lumbar vertebrae L1-4 bone density

[0223]

[0224] Table 4 Femoral neck bone density

[0225]

[0226] It can be seen from Table 3 and Table 4 that the Chinese medicinal preparation of the present invention can significantly improve the bone density of the three groups of lumbar vertebrae L1-4 and femoral neck.

[0227] The above description is only a preferred embodiment of the present invention, but the present invention is not limited to the scope of implementation shown. Any changes made in accordance with the concept of the present invention, or modifications to equivalent embodiments with equivalent changes, which still do not exceed the spirit covered by the specification, should be within the protection scope of the present invention.

Claims

1. A Chinese medicine preparation for treating osteoporosis, characterized in that: The composition comprises the following ingredients by weight: 15-18 parts of red vine, 3-5 parts of Kochia scoparia, 1-2 parts of Millettia reticulata, 10-12 parts of licorice, 1-3 parts of Poria, 2-4 parts of Cuscuta australis, 1-1.8 parts of Lithospermum officinale, 3-5 parts of Salvia miltiorrhiza complex, 1-3 parts of white peony root, 2-5 parts of Codonopsis pilosula, 4-6 parts of bitter almond, 2-3 parts of cinnamon bark, and 1-2 parts of corn starch.

2. A Chinese medicine preparation for treating osteoporosis according to claim 1, characterized in that: The preparation method of the Danshen complex is as follows: (1) First, pre-treat the salvia miltiorrhiza with a sodium chloride solution for 3-4 hours, then wash and drain to obtain pre-treated salvia miltiorrhiza; (2) mixing the pretreated salvia miltiorrhiza with water and beating the mixture to obtain salvia miltiorrhiza slurry; (3) first peeling the fresh pinellia to obtain peeled pinellia; The peeled pinellia tuber is irradiated with far infrared radiation for 2-5 minutes to obtain the pinellia tuber after enzyme inhibition; The irradiated pinellia tuber is subjected to microwave drying to obtain dried pinellia tuber; Grinding the dried pinellia tuber to obtain pinellia tuber powder; (4) adding the pinellia powder, salvia miltiorrhiza slurry, and water in sequence, and then stirring and mixing them evenly to obtain a mixed slurry; (5) decocting the mixed slurry for 2 hours to obtain a decocted mixed solution; (6) filtering the decoction mixture, concentrating the obtained filtrate, and obtaining the Danshen complex.

3. A Chinese medicine preparation for treating osteoporosis according to claim 2, characterized in that: The method for pre-treating Danshen in step (1) is: First, add Danshen to the sodium chloride solution, adjust the temperature to 40°C, let it stand, then filter and drain; Wherein, the mass fraction of the sodium chloride solution is 15%; The mixing ratio of Danshen and sodium chloride solution is 5g:120mL.

4. A Chinese medicine preparation for treating osteoporosis according to claim 2, characterized in that: The mixing ratio of the pretreated Danshen and water in step (2) is 10 g: 100 mL; Among them, the beating speed is 5000r / min.

5. A Chinese medicine preparation for treating osteoporosis according to claim 2, characterized in that: The irradiation intensity in step (3) far-infrared irradiation is 1100-1300W / m 2 .

6. A Chinese medicine preparation for treating osteoporosis according to claim 2, characterized in that: The mixing ratio of the pinellia powder, salvia miltiorrhiza slurry and water in step (4) is: 50-55g: 20-23g: 350-380mL.

7. A Chinese medicine preparation for treating osteoporosis according to claim 2, characterized in that: In step (5), the decoction is first boiled over high heat, then lowered to low heat and continued to be boiled.

8. A Chinese medicine preparation for treating osteoporosis according to claim 2, characterized in that: In step (6), the filtrate is concentrated to 1% of the original volume.

9. The method for preparing a Chinese medicine preparation for treating osteoporosis according to claim 1, characterized in that: The following steps are involved: S1 Preparation: Weigh the following components according to the mass ratio: red caulis rubra, Kochia scoparia, Millettia spatholobi, licorice, Poria, Cuscuta australis, Lithospermum officinale, salvia miltiorrhiza complex, white peony root, Codonopsis pilosula, bitter almond, cinnamon bark, corn starch; S2 material division: Divide each component into three groups: The first group includes: red vine, liquorice, Poria, dodder seed, bitter almond, cinnamon bark; The second group includes: Kochia scoparia, Millettia reticulata, Lithospermum officinale, White Peony Root, Codonopsis pilosula; The third group is: Danshen complex; S3 grouping extraction: For the first group, the raw materials of the first group were mixed in sequence, and then water of 5 times the total mass of the mixture was added, boiled over high heat, and then simmered over low heat for 2 hours, filtered, and concentrated to 5% of the original volume to obtain the first extract; For the second group, the second group of raw materials were mixed in sequence, and then an ethanol solution of 5 times the total mass of the mixture was added, and the mixture was soaked at room temperature for 10 hours, and then stirred for 2 hours, filtered to obtain a filtrate, and the operation was repeated twice on the filter residue, and the filtrate was combined, and the filtrate was decompressed at a temperature of 30°C to recover ethanol, and then concentrated to 5% of the original volume to obtain a second extract; S4: mixing the first extract, the second extract, and the Danshen complex together, adding corn starch, mixing, granulating, and drying to obtain a traditional Chinese medicine preparation; Dry at 50°C for 18 hours.

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