Novel microbial sludge reduction fungicide as well as preparation method and application thereof

Abstract

The invention discloses a novel microbial sludge reduction inoculant and a preparation method and application thereof, and belongs to the technical field of microorganisms, the microbial sludge reduction inoculant comprises a composite inoculant and a biomass additive; the complex microbial inoculant is prepared from the following components in parts by weight: 10 to 12 parts of lactic acid bacteria, 18 to 22 parts of saccharomycetes, 6 to 8 parts of bacillus licheniformis, 12 to 16 parts of bacillus subtilis, 4 to 6 parts of bacillus amyloliquefaciens, 7 to 9 parts of methane sarcina and 3 to 5 parts of horsehair methane bacteria; the biomass additive is fruit peel waste. According to the method disclosed by the invention, a biodegradation system with high activity of various required enzymes can be formed by utilizing the mutual action of microbial flora, dead or aged thalli in the activated sludge can be effectively destroyed and decomposed, meanwhile, organic matters can be decomposed, the sludge is subjected to reduction treatment under the condition that the effluent quality is not influenced, the treatment cost is low, and the treatment effect is good. The method is green and pollution-free and worthy of popularization.

Description

Technical Field

[0001] The present invention relates to the field of microbial technology, and in particular to a novel microbial sludge reducing agent and a preparation method and application thereof. Background Art

[0002] With the rapid economic development and the continuous acceleration of urbanization, the amount of sewage discharge has increased. If it is not treated in time, it will cause pollution and waste of water resources. Mud is rich in organic matter and nutrients such as nitrogen, phosphorus, and potassium, and is also considered a "misplaced resource." At the same time, the remaining sludge contains toxic and harmful substances. If it is not effectively treated and disposed of, it is easy to cause secondary pollution to the soil and groundwater, directly threatening public health and environmental safety. Up to now, my country's sludge treatment mainly adopts landfill, composting, natural drying and incineration, but these methods generally have the problems of high cost, serious environmental pollution, and immature technology. Therefore, an effective sludge treatment method is very important.

[0003] At present, activated sludge anaerobic digestion is the sludge treatment method used by most factories. Anaerobic digestion requires strict anaerobic conditions and relies on the synergistic action of complex microbial communities to convert organic matter into carbon dioxide (CO2) and methane (CH4). During the anaerobic digestion process, the organic particles in the sludge will undergo four stages: dissolution, hydrolysis, acidification, and methane production. Among them, dissolution and hydrolysis are considered to be the rate-limiting steps. The number of traditional microbial agents is relatively small, making it difficult to form a stable microbial colony. This results in the poor specificity of most traditional agents and unstable sludge reduction effects. In the process of reduction, they often only target the consumption of organic matter, and the degree of decomposition of extracellular polymers is not high. Therefore, the extracellular polymers in the sludge will wrap up the organic matter in the cells, making it difficult for the organic matter to be quickly utilized, resulting in a long hydrolysis phase cycle, poor results, and increased factory costs.

[0004] Prior art document 1 (CN109402003B) discloses a microbial agent for sludge reduction, comprising the following components in parts by weight: 8-10 parts of Clostridium acetobutylicum, 12-16 parts of Bacillus licheniformis, 16-20 parts of Bacillus subtilis, 5-7 parts of Lactobacillus acidophilus, 5-7 parts of Methanosarcina, 3-5 parts of Methanogenes bristle, 6-8 parts of Rhodopseudomonas palustris, 4-6 parts of Xanthomonas chlororaphis, 8-10 parts of Streptococcus thermophilus, 8-10 parts of Bacillus faecalis, 3-5 parts of Bacillus amyloliquefaciens, 2-4 parts of Nitrosomonas, 1-3 parts of Issatchengensis orientalis and 3-5 parts of Streptomyces bulls. However, its treatment cycle is too long, resulting in increased production costs and lower economic benefits. In addition, the addition of the agent requires a microbial carrier, which is not convenient. At the same time, the sludge reduction index is not clear enough, and it is difficult to reflect the reduction effect of the microbial reduction agent. Summary of the invention

[0005] In order to solve the deficiencies in the prior art, the present invention provides a microbial composite preparation and a method for reducing excess sludge. Treating sludge according to the strain ratio and sludge reduction method provided by the present invention can effectively solve the problems of long cycle and poor treatment effect in the anaerobic fermentation and hydrolysis stage of sludge.

[0006] The present invention adopts the following technical solution.

[0007] The first aspect of the present invention discloses a novel microbial sludge reduction agent, comprising a biomass additive and a composite agent.

[0008] The composite bacterial agent comprises: yeast, Bacillus subtilis, Bacillus amyloliquefaciens, lactic acid bacteria, Bacillus licheniformis, Methanogenes bristle and Methanosarcina;

[0009] The biomass additive is fruit peel waste;

[0010] The microbial sludge reduction agent is mixed according to the following components in parts by weight: 10-12 parts of biomass additives, 10-12 parts of lactic acid bacteria, 18-22 parts of yeast, 6-8 parts of Bacillus licheniformis, 12-16 parts of Bacillus subtilis, 4-6 parts of Bacillus amyloliquefaciens, 7-9 parts of Methanosarcina, and 3-5 parts of Methanogenes.

[0011] The second aspect of the present invention discloses a method for preparing a novel microbial sludge reducing agent, based on the novel microbial sludge reducing agent, comprising the following steps:

[0012] Preparation of biomass additives: adding peel waste into preheated alkali solution, soaking, taking out and washing, heating to constant weight, crushing and sieving to obtain biomass additive powder;

[0013] Preparation of microbial agents: inoculating the composite agent into a culture medium, culturing under set conditions, obtaining wet bacteria, pre-freezing a sample, and freeze-drying the sample to obtain a dried solid bacterial powder;

[0014] The prepared solid bacterial powder and biomass auxiliary agent powder are mixed according to a weight ratio to obtain a novel microbial sludge reduction agent.

[0015] Preferably, the preparation of the biomass additive specifically includes: taking peel waste, washing it with clean water for standby use, preheating the prepared alkali solution, adding the peel waste into the alkali solution, soaking it, taking it out and washing it with clean water, putting it in an oven and heating it to constant weight, crushing it with a grinder and sieving it to obtain biomass additive powder.

[0016] Preferably, the alkali solution is a sodium hydroxide solution with a concentration of 8-14%.

[0017] Preferably, the preparation of the microbial agent specifically includes:

[0018] Inoculate yeast, Bacillus subtilis, Bacillus amyloliquefaciens, lactic acid bacteria, Bacillus licheniformis, Methanogenes bristle, and Methanosarcina into the culture medium respectively;

[0019] Cultivate under set conditions, centrifuge each culture medium to obtain wet bacteria, add 20% glycerol as protective solution, and prefreeze at -90°C for at least 12h;

[0020] After the pre-frozen sample is taken out, the sample is freeze-dried to obtain a dried solid bacterial powder.

[0021] Preferably, the components of the culture medium include 8-12 g / L glucose, 6-8 g / L L-glutamic acid, 3-5 g / L ammonium sulfate, 4-6 g / L calcium phosphate, 0.1 g / L potassium chloride, 0.6 g / L ferric chloride, and 0.8 g / L anhydrous copper sulfate.

[0022] Preferably, the set culture condition is to expand the culture at 30-35° C. for at least 120 hours.

[0023] The third aspect of the present invention discloses the application of the novel microbial sludge reducing agent for sludge reduction in a sewage treatment plant.

[0024] Preferably, the novel microbial weight-reducing agent is added to the sludge anaerobic fermentation device tank of the sewage treatment plant and stirred after anaerobic treatment.

[0025] Preferably, the dosage of the novel microbial weight-reducing agent is 0.2-0.3 g / g TSS.

[0026] Compared with the prior art, the beneficial effects of the present invention include at least:

[0027] The number of traditional microbial agents is too small to form a stable microbial colony, which results in poor specificity and unstable sludge reduction effect. In the process of sludge reduction, they often only target the consumption of organic matter and have a low degree of decomposition of extracellular polymers, resulting in a long hydrolysis cycle and increased factory costs. In view of these shortcomings, the present invention adopts a new type of microbial agent based on yeast and bacillus.

[0028] The bacterial agent can effectively hydrolyze the extracellular polymers of sludge, so that large molecular organic matter can be released and decomposed into small molecular organic matter. Small molecular organic matter can also be used by lactic acid bacteria to produce acid. As the sludge fermentation proceeds, the CO2 concentration and acetic acid content in the system increase, and the dominant bacteria gradually change from yeast to methanogens dominated by Methanosarcina. Methanosarcina can use CO2 and acetic acid to produce methane gas to achieve the effect of reduction.

[0029] The novel bacterial agent mentioned in the present invention can utilize the mutualism between microbial flora to form a biodegradation system with high required enzyme activities, which can effectively destroy and decompose dead or aged bacteria in activated sludge, and at the same time can decompose organic matter. Without affecting the effluent quality, the amount of residual sludge produced can be reduced by reducing the amount of sludge in the process.

[0030] The use of this new microbial agent can achieve a sludge reduction rate of more than 30% during sewage treatment without significantly changing the current sewage treatment process, thereby reducing the comprehensive operating cost of sludge treatment, which is of great significance in terms of economic, environmental and social benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is the relative abundance diagram of species at the sludge phylum level under the regulation of the novel sludge microbial reduction agent in the present invention, Y1 is the original sludge, and F1 is regulated by the biomass agent;

[0032] Figure 2 This is a scanning electron microscope image of the sludge after the novel sludge microbial reducing agent is added in the present invention. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical scheme and advantages of the present invention clearer, the technical scheme of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. The embodiments described in this application are only embodiments of a part of the present invention, rather than all embodiments. Based on the spirit of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work belong to the protection scope of the present invention.

[0034] The purpose of the present invention is to provide a microbial composite preparation and a method for reducing excess sludge. Treating sludge according to the bacterial strain ratio and sludge reduction method provided by the present invention can effectively solve the problem of long anaerobic fermentation and hydrolysis period of sludge and poor treatment effect.

[0035] Embodiment 1 of the present invention provides a novel microbial sludge reduction agent, which is composed of a biomass additive and a composite agent.

[0036] The composite bacterial agent includes yeast, Bacillus subtilis, Bacillus amyloliquefaciens, lactic acid bacteria, Bacillus licheniformis, Methanogenes bristle, and Methanosarcina;

[0037] Biomass additives include fruit peel waste, which contains limonene, flavanones and fructose.

[0038] The following weight portions of the bacterial powders of each component are fully mixed: 10-12 parts of biomass additives, 10-12 parts of lactic acid bacteria, 18-22 parts of yeast, 6-8 parts of Bacillus licheniformis, 12-16 parts of Bacillus subtilis, 4-6 parts of Bacillus amyloliquefaciens, 7-9 parts of Methanosarcina, and 3-5 parts of Methanogenes.

[0039] In the early stage of anaerobic fermentation of sludge, yeast and Bacillus subtilis can secrete α-amylase and protease to hydrolyze the extracellular polymers in the sludge flocs, release intracellular substances (mainly proteins, fats and carbohydrates, etc.) and various hydrolases, increase the contact between enzymes and substrates, and accelerate the hydrolysis process. Small molecular organic matter can also be used by lactic acid bacteria to produce acid.

[0040] As the sludge fermentation progresses, the CO2 concentration and acetic acid content in the system increase, and the dominant bacteria gradually change from yeast to methanogens dominated by Methanosarcina. Under anaerobic conditions, methanogens can use CO2 and acetic acid to produce methane gas. The concentration of organic matter and solids in the entire system began to decrease significantly. At the same time, Bacillus subtilis reacts with ammonium ions with nitrate as an electron donor to produce methane under anaerobic conditions, thereby achieving the effect of reducing sludge.

[0041] Embodiment 2 of the present invention provides a method for preparing a novel microbial sludge reducing agent, comprising the following steps:

[0042] Step 1, preparation of biomass additive: adding the peel into preheated alkali solution, soaking, taking out and washing, heating to constant weight, crushing and sieving to obtain biomass additive powder;

[0043] In a preferred but non-limiting embodiment of the present invention, it specifically includes: taking 50g of peel waste, washing it with clean water for standby use, preparing sodium hydroxide solution, heating the prepared alkali solution to 90°C, adding the peel waste to the preheated alkali solution, soaking it for 1 hour, taking it out and washing it with clean water, putting it in a 105°C oven and heating it for 24 hours to constant weight, crushing it with a grinder and passing it through a 60-mesh sieve to obtain biomass additive powder.

[0044] Further preferably, the concentration of the sodium hydroxide is 8-14%.

[0045] Step 2, preparation of microbial inoculants: inoculating the composite inoculant into a culture medium, culturing under set conditions, obtaining wet bacterial cells, pre-freezing a sample, and freeze-drying the sample to obtain a dried solid bacterial powder;

[0046] Specifically include:

[0047] Step 2.1, inoculating yeast, Bacillus subtilis, Bacillus amyloliquefaciens, lactic acid bacteria, Bacillus licheniformis, Methanogenes pubescens, and Methanosarcina into the culture medium respectively;

[0048] In a preferred but non-limiting embodiment of the present invention, the components of the culture medium include 8-12 g / L glucose, 6-8 g / L L-glutamic acid, 3-5 g / L ammonium sulfate, 4-6 g / L calcium phosphate, 0.1 g / L potassium chloride, 0.6 g / L ferric chloride, and 0.8 g / L anhydrous copper sulfate.

[0049] Preferred are 8 g / L of glucose, 6 g / L of L-glutamic acid, 3 g / L of ammonium sulfate, 4 g / L of calcium phosphate, 0.1 g / L of potassium chloride, 0.6 g / L of ferric chloride, and 0.8 g / L of anhydrous copper sulfate.

[0050] Further preferably, the culture medium may also include 6-8 g / L yeast extract, 12-14 g / L peptone, 12-16 g / L glucose, 0.1-0.5 g / L potassium chloride, 0.6-0.8 g / L ferric chloride, and 0.4-0.8 g / L anhydrous copper sulfate. Agar (10-16 g / L) may be appropriately added to the formula.

[0051] Step 2.2, culture under set conditions, centrifuge each culture medium to obtain wet bacteria, add 20% glycerol as a protective solution, and pre-freeze at -90°C for 12h;

[0052] The set condition is to expand the culture at 30-35°C for 120 hours.

[0053] Step 2.3, after taking out the pre-frozen sample, freeze-dry the sample using a vacuum freeze dryer to obtain a dried solid bacterial powder.

[0054] Step 3, mixing the prepared solid bacterial powder and biomass auxiliary agent powder according to the weight ratio: 10-12 parts of biomass auxiliary agent, 10-12 parts of lactic acid bacteria, 18-22 parts of yeast, 6-8 parts of Bacillus licheniformis, 12-16 parts of Bacillus subtilis, 4-6 parts of Bacillus amyloliquefaciens, 7-9 parts of Methanosarcina, and 3-5 parts of Methanogenes to obtain a new microbial sludge reduction agent.

[0055] The new microbial species and the optimal compound ratio of microorganisms in the novel microbial sludge reduction agent can achieve the cooperation of various strains to promote anaerobic digestion of sludge, determine the optimal ratio of strains, and then the cycle is mainly 10 days. MLSS and VSS can be measured to characterize the degree of reduction and COD to characterize the organic matter content, and the relative abundance of microbial fungi structure and sludge morphology after treatment with the microbial reduction agent can be detected. Figure 1-2 shown.

[0056] from Figure 1It can be seen that compared with the original sludge Y1, the proportion of Firmicutes in the fermented sludge F1 under the regulation of biomass agents increased significantly, from the initial 0.87% to 59.2%, while the abundance of Proteobacteria decreased significantly, from the initial 21.7% to 11.9%; this shows that the introduction of biomass agents will significantly change the sludge microbial colony structure, and the significant increase in the abundance of Firmicutes can effectively break the rate-limiting step of anaerobic fermentation of sludge, promote the dissolution of intracellular substances in sludge and accelerate the hydrolysis and acidification process of organic matter; the decrease in the abundance of Proteobacteria can slow down the degradation of acetate, the main effective component of the carbon source of the fermentation liquid, by microorganisms inside the sludge, thereby increasing the proportion of acetic acid in the sludge fermentation liquid. Therefore, under the synergistic effect of the two, the sludge colony structure is transformed in a direction that is conducive to the generation of carbon sources, which significantly improves the acid production effect of anaerobic fermentation of sludge.

[0057] from Figure 2 It can be seen that the surface structure of the sludge after the microbial reduction agent is incomplete, the spherical structure is reduced, and the granular material is reduced, indicating that the treatment has caused a significant change in the sludge structure. The hydrolytic enzymes secreted by the microorganisms are conducive to the destruction of the microbial cell wall structure, promoting the release of intracellular substances, and providing acid-producing substrates for the subsequent anaerobic fermentation of sludge to prepare carbon sources. There are large sludge flocs in the original sludge, but after pretreatment with the microbial reduction agent, the sludge flocs are significantly smaller and the sludge particles become uniform, indicating that the microbial agent treatment can promote the solubilization effect of sludge and have a positive effect on anaerobic fermentation.

[0058] Example 3 of the present invention provides an application of a novel microbial sludge reduction agent.

[0059] Sewage treatment experiment 1 of Example 3

[0060] New microbial reduction agent 1:

[0061] The following weight portions of the bacterial powders of each component are fully mixed: 10 parts of biomass additives, 10 parts of lactic acid bacteria, 18 parts of yeast, 6 parts of Bacillus licheniformis, 12 parts of Bacillus subtilis, 4 parts of Bacillus amyloliquefaciens, 7 parts of Methanosarcina, and 3 parts of Methanogenes; set aside.

[0062] A sewage treatment plant in Dongying District, Dongying City was selected, and the secondary sedimentation tank pipe was connected to the sludge anaerobic fermentation device tank. 3000L of sludge was added with a new microbial reduction agent according to 0.2g / gTSS, and stirred after anaerobic treatment. At the same time, a blank control group was set up, and the main sewage reduction indicators were tested after 10 days of reaction.

[0063] The main indicators include sludge concentration, i.e. mixed liquor suspended solids concentration (MLVSS), chemical oxygen demand (COD), ammonia nitrogen and total nitrogen, as shown in Table 1 below.

[0064] Table 1 Comparison of data before and after sewage treatment

[0065]

[0066] From the above examples, it can be seen that the present invention, by compounding different microbial agents, makes full use of the mutualism between microbial flora to form a biodegradation system with high enzyme activities, thus achieving a good reduction effect. Moreover, the method is simple to operate, green and pollution-free, has high economic benefits, and has obvious treatment effects.

[0067] Sewage treatment experiment 2 of Example 3

[0068] New microbial reduction agent 2:

[0069] The following weight portions of the bacterial powders of each component are fully mixed: 12 parts of biomass additives, 11 parts of lactic acid bacteria, 22 parts of yeast, 8 parts of Bacillus licheniformis, 16 parts of Bacillus subtilis, 6 parts of Bacillus amyloliquefaciens, 8 parts of Methanosarcina, and 5 parts of Methanogenes; set aside.

[0070] A sewage treatment plant in Dongying District, Dongying City was selected, and the secondary sedimentation tank pipe was connected to the sludge anaerobic fermentation device tank. 3000L of sludge was added with the new microbial reduction agent 2 according to 0.2g / gTSS, and stirred after anaerobic treatment. At the same time, a blank control group was set up, and the main sewage reduction indicators were tested after 10 days of reaction.

[0071] The main indicators include sludge concentration, i.e. mixed liquor suspended solids concentration (MLVSS), chemical oxygen demand (COD), ammonia nitrogen and total nitrogen, as shown in Table 2 below.

[0072] Table 2 Comparison of data before and after sewage treatment

[0073]

[0074] From the above examples, it can be seen that the present invention, by compounding different microbial agents, makes full use of the mutualism between microbial flora to form a biodegradation system with high enzyme activities, thus achieving a good reduction effect. Moreover, the method is simple to operate, green and pollution-free, has high economic benefits, and has obvious treatment effects.

[0075] It is worth noting that the dosage can be 0.2-0.3 g / g TSS. A larger dosage will increase the cost of the microbial agent, so 0.2 g / g TSS is preferably used.

[0076] Compared with the prior art, the beneficial effects of the present invention include at least:

[0077] The number of traditional microbial agents is too small to form a stable microbial colony, which results in poor specificity and unstable sludge reduction effect. In the process of sludge reduction, they often only target the consumption of organic matter and have a low degree of decomposition of extracellular polymers, resulting in a long hydrolysis cycle and increased factory costs. In view of these shortcomings, the present invention adopts a new type of microbial agent based on yeast and bacillus.

[0078] The bacterial agent can effectively hydrolyze the extracellular polymers of sludge, so that large molecular organic matter can be released and decomposed into small molecular organic matter. Small molecular organic matter can also be used by lactic acid bacteria to produce acid. As the sludge fermentation proceeds, the CO2 concentration and acetic acid content in the system increase, and the dominant bacteria gradually change from yeast to methanogens dominated by Methanosarcina. Methanosarcina can use CO2 and acetic acid to produce methane gas to achieve the effect of reduction.

[0079] The novel bacterial agent mentioned in the present invention can utilize the mutualism between microbial flora to form a biodegradation system with high required enzyme activities, which can effectively destroy and decompose dead or aged bacteria in activated sludge, and at the same time can decompose organic matter. Without affecting the effluent quality, the amount of residual sludge produced can be reduced by reducing the amount of sludge in the process.

[0080] The use of this new microbial agent can achieve a sludge reduction rate of more than 30% during sewage treatment without significantly changing the current sewage treatment process, thereby reducing the comprehensive operating cost of sludge treatment, which is of great significance in terms of economic, environmental and social benefits.

[0081] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the relevant field should understand that the specific implementation methods of the present invention can still be modified or replaced by equivalents, and any modifications or equivalent replacements that do not depart from the spirit and scope of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A novel microbial sludge reduction agent, comprising a biomass additive and a composite agent, characterized in that: The composite bacterial agent comprises: yeast, Bacillus subtilis, Bacillus amyloliquefaciens, lactic acid bacteria, Bacillus licheniformis, Methanogenes bristle and Methanosarcina; The biomass additive is fruit peel waste; The microbial sludge reduction agent is mixed according to the following components in parts by weight: 10-12 parts of biomass additives, 10-12 parts of lactic acid bacteria, 18-22 parts of yeast, 6-8 parts of Bacillus licheniformis, 12-16 parts of Bacillus subtilis, 4-6 parts of Bacillus amyloliquefaciens, 7-9 parts of Methanosarcina, and 3-5 parts of Methanogenes.

2. A method for preparing a novel microbial sludge reducing agent, based on the novel microbial sludge reducing agent according to claim 1, characterized in that: The following steps are involved: Preparation of biomass additives: adding peel waste into preheated alkali solution, soaking, taking out and washing, heating to constant weight, crushing and sieving to obtain biomass additive powder; Preparation of microbial agents: inoculating the composite agent into a culture medium, culturing under set conditions, obtaining wet bacteria, pre-freezing a sample, and freeze-drying the sample to obtain a dried solid bacterial powder; The prepared solid bacterial powder and biomass auxiliary agent powder are mixed according to a weight ratio to obtain a novel microbial sludge reduction agent.

3. The method for preparing a novel microbial sludge reducing agent according to claim 2, characterized in that: The preparation of the biomass additive specifically includes: taking peel waste, washing it with clean water for standby use, preheating the prepared alkali solution, adding the peel waste into the alkali solution, soaking it, taking it out and washing it with clean water, putting it in an oven and heating it to constant weight, crushing it with a grinder and sieving it to obtain biomass additive powder.

4. The method for preparing a novel microbial sludge reducing agent according to claim 3, characterized in that: The alkali solution is a sodium hydroxide solution with a concentration of 8-14%.

5. The method for preparing a novel microbial sludge reducing agent according to claim 2, characterized in that: The preparation of the microbial agent specifically includes: Inoculate yeast, Bacillus subtilis, Bacillus amyloliquefaciens, lactic acid bacteria, Bacillus licheniformis, Methanogenes bristle, and Methanosarcina into the culture medium respectively; Cultivate under set conditions, centrifuge each culture medium to obtain wet bacteria, add 20% glycerol as protective solution, and prefreeze at -90°C for at least 12h; After the pre-frozen sample is taken out, the sample is freeze-dried to obtain a dried solid bacterial powder.

6. The method for preparing a novel microbial sludge reducing agent according to claim 5, characterized in that: The components of the culture medium include 8-12 g / L glucose, 6-8 g / L L-glutamic acid, 3-5 g / L ammonium sulfate, 4-6 g / L calcium phosphate, 0.1 g / L potassium chloride, 0.6 g / L ferric chloride, and 0.8 g / L anhydrous copper sulfate.

7. The method for preparing a novel microbial sludge reducing agent according to claim 5, characterized in that: The set culture condition is to expand the culture at 30-35° C. for at least 120 hours.

8. Use of the novel microbial sludge reduction agent according to claim 1 for reducing sludge in a sewage treatment plant.

9. The use of a novel microbial sludge reduction agent according to claim 8, characterized in that: The novel microbial weight-reducing agent is added to the sludge anaerobic fermentation device tank of the sewage treatment plant and stirred after anaerobic treatment.

10. The use of a novel microbial sludge reduction agent according to claim 8, characterized in that: The dosage of the novel microbial weight-reducing agent is 0.2-0.3 g / g TSS.

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