A strain of P. lilacensis and its application

A technology of P. lilacinus and biological agent, applied in the field of bioengineering, can solve the problems of tobacco planting soil and environmental damage, and achieve the effects of improving tobacco agronomic properties, increasing tobacco yield, and being environmentally friendly

Active Publication Date: 2020-05-19
广州市博仕奥生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many factors that affect the yield of tobacco planting, including climate, tobacco species, soil, fertilization, pests and diseases, etc. In order to increase tobacco production, a large amount of chemical fertilizers and pesticides are used in the planting process, which accumulate over time and cause damage to the soil and environment of tobacco planting. havoc

Method used

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  • A strain of P. lilacensis and its application
  • A strain of P. lilacensis and its application
  • A strain of P. lilacensis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Seed medium: sucrose 30g / L, NaNO 3 3g / L, K 2 HPO 4 ·7H 2 O 1g / L, MgSO 4 ·7H 2 O 0.5g / L, KCl 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, the solvent is water.

[0036] Fermentation medium: sucrose 30g / L, soybean powder 4g / L, K 2 HPO 4 ·7H 2 O 3g / L, MgSO 4 ·7H 2 O 0.2g / L, FeSO 4 ·7H 2 O 0.01g / L, solvent is water, pH 7.0.

[0037] Inoculate the activated Purpureocillium lilacinum (Purpureocillium lilacinum) BPT05 with the preservation number GDMCC NO: 60178 in the seed medium, 28°C, 180r / min shaker culture for 24h to obtain the seed liquid, and then press the inoculation amount of 10% (v / v) Inoculated into the fermentation medium, 28 ~ 32 ° C, 200r / min shaker fermentation culture for 96h, the fermentation broth was diluted 20 times, and the 20 times diluted fermentation medium was used as a control. The test seeds were coated seeds of Yunyan 87, refer to GB / T 3543.4-1995 carries out germination test, and the results are shown in Table 1. As can be seen from Table 1, t...

Embodiment 2

[0041] Seed medium: sucrose 30g / L, NaNO 3 3g / L, K 2 HPO 4 ·7H 2 O 1g / L, MgSO 4 ·7H 2 O 0.5g / L, KCl 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, the solvent is water.

[0042] Fermentation medium: sucrose 25g / L, crushed bran 5g / L, NaNO 3 1g / L, K 2 HPO 4 ·7H 2 O 4g / L, MgSO 4 ·7H 2 O 0.2g / L, FeSO 4 ·7H 2 O 0.01g / L, solvent is water, pH 7.5.

[0043] Fermentation: inoculate activated Purpureocillium lilacinum (Purpureocillium lilacinum) BPT05 with the preservation number GDMCC NO: 60178 in the seed medium, 28°C, 200r / min shaker culture for 24h to obtain the seed liquid, and then press 10% (v / v) The inoculum was inoculated into the fermentation medium, the fermentation temperature was 30°C, the aeration was 1.0vvm, the stirring speed was 300r / min, the fermentation was 96h, and the fermentation broth was harvested.

[0044] Post-processing: add 200g of rice husk powder, 50g of white carbon black, 5g of xanthan gum, and 1g of sodium citrate to each liter of fermentation broth, s...

Embodiment 3

[0046] Seed medium: sucrose 30g / L, NaNO 3 3g / L, K 2 HPO 4 ·7H 2 O 1g / L, MgSO 4 ·7H 2 O 0.5g / L, KCl 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, the solvent is water.

[0047] Fermentation medium: sucrose 30g / L, corn flour 5g / L, K 2 HPO 4 ·7H 2 O 4g / L, MgSO 4 ·7H 2 O 0.3g / L, FeSO 4 ·7H 2 O 0.01g / L, solvent is water, pH 7.2.

[0048] Fermentation: inoculate activated Purpureocillium lilacinum (Purpureocillium lilacinum) BPT05 with the preservation number GDMCC NO: 60178 in the seed medium, 28°C, 200r / min shaker culture for 24h to obtain the seed liquid, and then press 10% (v / v) The inoculum was inoculated into the fermentation medium, the fermentation temperature was 28°C, the aeration was 0.8vvm, the stirring speed was 300r / min, the fermentation was 72h, and the fermentation broth was harvested.

[0049] Post-processing: add 200 g of crushed bagasse, 5 g of glycerin, and 1 g of sodium citrate to each liter of fermented liquid, stir and mix for 30 minutes, and dry by drum to ...

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Abstract

The invention discloses a strain of P. lilacinus and its application. The strain is Purpureocillium lilacinum (Purpureocillium lilacinum) BPT05, which was preserved on May 09, 2017 in the Guangdong Provincial Microbiological Culture Collection Center on the 5th Floor, Building 59, Compound 59, No. 100 Xianlie Middle Road, Guangzhou City. Deposit number: GDMCC NO: 60178. During the process of growth and metabolism, the bacterial strain produces substances that can promote plant growth, especially significantly promote the growth of tobacco. Using the strain for liquid fermentation, and then post-processing the fermentation product, it can be prepared into a tobacco growth-promoting biological bacterial agent. The tobacco growth-promoting biological bacterial agent is convenient for storage and transportation, easy to use, stable in activity, and can effectively improve the agronomic properties of tobacco. It can increase tobacco production; it is environmentally friendly and has no pollution to soil and environment, which is of great significance to the sustainable development of agriculture.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing a strain of P. lilacensis and a tobacco growth-promoting biological bacterial agent and its application. Background technique [0002] Tobacco is one of the important economic crops in China. In my country, the total tobacco output accounts for about 1 / 3 of the world's tobacco output, ranking first in the world. The tobacco industry has been a large profit and tax household for many years and an important economic source of national and local taxes. There are many factors that affect the yield of tobacco planting, including climate, tobacco species, soil, fertilization, pests and diseases, etc. In order to increase tobacco production, a large amount of chemical fertilizers and pesticides are used in the planting process, which accumulate over time and cause damage to the soil and environment of tobacco planting. severe damage. [0003] B...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P21/00C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 廖美德梁锏文杜景德陈泽鹏
Owner 广州市博仕奥生物科技有限公司
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