Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fermentation culture medium and culture method for increasing leucinostatin produced from purpureocillium lilacinum

A fermentation medium, the technology of P. lilacinus, which is applied in the field of fermentation medium for improving the production of P. lilacin, to achieve the effects of increasing yield, shortening the fermentation period, and achieving a balanced and reasonable composition ratio.

Active Publication Date: 2017-07-21
河南省农业科学院园艺研究所 +1
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the current prior art, there are still technical problems to be solved urgently in the rapid propagation of the P.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: A kind of fermented medium PPDB that improves P. lilacinus to produce gystatin, contains the raw material of following concentration: glucose 50.0g / L, peptone 5.0g / L, yeast extract 5g / L, K 2 HPO 4 1.0g / L, Na 2 CO 3 10.0g / L, MgSO 4 ·7H 2 O 0.2g / L, corn flour 3g / L, FeSO 4 ·7H 2 O 0.047g / L and urea 5g / L.

Embodiment 2

[0028] Embodiment 2: a kind of fermented medium PPDB that improves P. lilacinus to produce gystatin, comprises the raw material of following weight portion: glucose 55 g / L, peptone 4.5 g / L, yeast extract 5.5 g / L, K 2 HPO 4 0.5 g / L, Na 2 CO 3 12.5g / L, MgSO 4 ·7H 2 O 0.1g / L, corn flour 10 g / L, FeSO 4 ·7H 2 O 0.005 g / L and urea 10.5 g / L.

Embodiment 3

[0029] Embodiment 3: a kind of fermented medium PPDB that improves P. lilacinus to produce gystatin, comprises the raw material of following weight portion: glucose 45 g / L, peptone 5.5 g / L, yeast extract 4.5 g / L, K 2 HPO 4 2.0 g / L, Na 2 CO 3 8.5g / L, MgSO 4 ·7H 2 O 0.5 g / L, corn flour 1.5 g / L, FeSO 4 ·7H 2 O 0.05 g / L and urea 1.5 g / L.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a fermentation culture medium for increasing leucinostatin produced from purpureocillium lilacinum. The fermentation culture medium comprises, by weight, 45-55 parts of glucose, 4.5-5.5 parts of peptone, 4.5-5.5 parts of yeast extract, 0.5-2.0 parts of K2HPO4, 8.5-12.5 parts of Na2CO3, 0.1-0.5 part of MgSO4.7H2O, 1.5-10 parts of corn flour, 0.005-0.05 part of FeSO4.7H2O and 1.5-10.5 parts of urea. A culture method for increasing the leucinostatin produced from the purpureocillium lilacinum by the aid of the fermentation culture medium includes steps of carrying out fermentation culture on culture bacterium solution by the aid of purpureocillium lilacinum shake flasks; carrying out culture on the culture bacterium solution in seed tanks by the aid of the fermentation culture medium; inoculating the culture bacterium solution in the seed tanks into fermentation tanks and carrying out fermentation. The fermentation culture medium and the culture method have the advantages that the purpureocillium lilacinum cultured by the aid of the fermentation culture medium is low in cost, short in fermentation cycle and high in leucinostatin yield, and the like.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a fermentation medium and a cultivation method for improving the production of pyristatin by P. lilacinus. Background technique [0002] Cucumber root-knot nematode ( Meloidogyne spp. ) belongs to the genus Cucumber root-knot nematodes ( Meloidogyne Goeldi), is a kind of important plant pathogenic nematodes that seriously harm commercial crops and are widely distributed all over the world. Cucumber root-knot nematode has a very wide host range. It can infect not only economical food crops, vegetables and ornamental flowers and trees, but even weeds. Cucumber root-knot nematode disease can reduce the yield of commercial crops by 10-20%, and in severe cases, it can reach more than 75%, and even lead to crop failure. In my country, due to the rapid development of the agricultural economy, the continuous reform and adjustment of the industrial structure, the con...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12R1/645
CPCC07K7/06C12P21/02
Inventor 杨凡史宣杰马凯蔡毓新赵秀山赵肖斌唐艳领王琰
Owner 河南省农业科学院园艺研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com