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Fermentation medium and culture method for improving P. lilacin production

A technology of P. lilacinus and peonystatin, which is applied in the field of fermentation medium for improving P. lilacinum producing peonystatin

Active Publication Date: 2021-04-23
河南省农业科学院园艺研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the current prior art, there are still technical problems to be solved urgently in the rapid propagation of the P.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: A kind of fermented medium PPDB that improves P. lilacinus to produce gystatin, contains the raw material of following concentration: glucose 50.0g / L, peptone 5.0g / L, yeast extract 5g / L, K 2 HPO 4 1.0g / L, Na 2 CO 3 10.0g / L, MgSO 4 ·7H 2 O 0.2g / L, corn flour 3g / L, FeSO 4 ·7H 2 O 0.047g / L and urea 5g / L.

Embodiment 2

[0028] Embodiment 2: a kind of fermented medium PPDB that improves P. lilacinus to produce gystatin, comprises the raw material of following weight portion: glucose 55 g / L, peptone 4.5 g / L, yeast extract 5.5 g / L, K 2 HPO 4 0.5 g / L, Na 2 CO 3 12.5g / L, MgSO 4 ·7H 2 O 0.1g / L, corn flour 10 g / L, FeSO 4 ·7H 2 O 0.005 g / L and urea 10.5 g / L.

Embodiment 3

[0029] Embodiment 3: a kind of fermented medium PPDB that improves P. lilacinus to produce gystatin, comprises the raw material of following weight portion: glucose 45 g / L, peptone 5.5 g / L, yeast extract 4.5 g / L, K 2 HPO 4 2.0 g / L, Na 2 CO 3 8.5g / L, MgSO 4 ·7H 2 O 0.5 g / L, corn flour 1.5 g / L, FeSO 4 ·7H 2 O 0.05 g / L and urea 1.5 g / L.

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Abstract

The invention relates to a fermentation medium for improving the production of pylorystatin by P. lilacinus, which comprises the following raw materials in parts by weight: 45-55 parts of glucose, 4.5-5.5 parts of peptone, 4.5-5.5 parts of yeast extract, K 2 HPO 4 0.5~2.0 parts, Na 2 CO 3 8.5~12.5 parts, MgSO 4 ·7H 2 O 0.1-0.5 parts, corn flour 1.5-10 parts, FeSO 4 ·7H 2 O 0.005-0.05 parts and urea 1.5-10.5 parts. The cultivation method of using the culture medium to improve the production of P. lilacinus by P. lilacinus comprises the following steps: fermenting and culturing the shake flask culture liquid of P. lilacinus, using the fermentation medium of the present invention to carry out seed tank culture, cultivating the seed tank Bacterial liquid inoculates the fermenter for fermentation. Adopting the fermentation medium of the present invention to cultivate the P. lilacinus has the characteristics of low cost, short fermentation cycle, high yield of lignostatin, and the like.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a fermentation medium and a cultivation method for improving the production of pyristatin by P. lilacinus. Background technique [0002] Cucumber root-knot nematode ( Meloidogyne spp. ) belongs to the genus Cucumber root-knot nematodes ( Meloidogyne Goeldi), is a kind of important plant pathogenic nematodes that seriously harm commercial crops and are widely distributed all over the world. Cucumber root-knot nematode has a very wide host range. It can infect not only economical food crops, vegetables and ornamental flowers and trees, but even weeds. Cucumber root-knot nematode disease can reduce the yield of commercial crops by 10-20%, and in severe cases, it can reach more than 75%, and even lead to crop failure. In my country, due to the rapid development of the agricultural economy, the continuous reform and adjustment of the industrial structure, the con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC07K7/06C12P21/02
Inventor 杨凡史宣杰马凯蔡毓新赵秀山赵肖斌唐艳领王琰
Owner 河南省农业科学院园艺研究所
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