Microorganism-plant two-way insect-resistant fungicide and preparation method thereof

A microorganism and plant technology, applied in the fields of botanical equipment and methods, biocides, plant growth regulators, etc., can solve the problems of poor insecticidal effect, narrow bactericidal spectrum, unstable mixture, etc., and achieve a wide range of effects. , the effect of wide antibacterial spectrum and low production cost

Inactive Publication Date: 2021-07-27
黎昊源生物工程(山东)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many types of biopesticides, which are mainly divided into three categories: microorganisms, insect viruses, and plant extracts; among them, microorganisms and plant extracts are the most commonly used, and they have the advantages of convenient material acquisition, low cost, and long control period. Efficient, economical, safe, non-polluting, and highly compatible with the environment, it is the best choice for the production of pollution-free green vegetables; There are many plant extract insecticides; if used alone, the insecticidal effect will be poor and the bactericidal spectrum will be narrow; if microbial insecticides and plant extract insecticides are used directly in combination, there will be When the mixture is unstable

Method used

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  • Microorganism-plant two-way insect-resistant fungicide and preparation method thereof
  • Microorganism-plant two-way insect-resistant fungicide and preparation method thereof
  • Microorganism-plant two-way insect-resistant fungicide and preparation method thereof

Examples

Experimental program
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Effect test

preparation example 1

[0050] Preparation Example 1: The shake flask liquid culture medium of Bacillus thuringiensis is prepared by the following method:

[0051] Weigh 3g of beef extract, 10g of peptone, 5g of sodium chloride, 20g of agar, and 1000g of water, mix and stir for 15 minutes, adjust the pH to 7.2, and sterilize at 121°C and 0.1Mpa for 30 minutes to prepare a shaker flask liquid culture medium for Bacillus thuringiensis .

preparation example 2

[0052] Preparation Example 2: The shake flask liquid culture medium of Beauveria bassiana and Porphyra lilacinus is prepared by the following method:

[0053] Weigh 200g of potatoes, 20g of glucose, 20g of agar, 1000g of water, peel the potatoes and cut them into about 2cm 2 Then put it into a 1500mL beaker and boil for 30min, filter and take the filtrate, then add distilled water to make up to 1000mL, and sterilize at 121°C and 0.1Mpa for 30min to prepare the shake flask liquid culture medium of Beauveria bassiana.

preparation example 3

[0054] Preparation Example 3: The fermenter liquid medium of Bacillus thuringiensis, Beauveria bassiana and Porphyra lilacinus were all prepared by the following method:

[0055] Weigh soybean protein powder 4kg, sucrose 4kg, corn starch 3kg, potassium dihydrogen phosphate 0.8kg, dipotassium hydrogen phosphate 0.2kg, peptone 4kg, yeast powder 0.3kg, beef extract 2kg, glucose 3kg, trace elements 0.8kg, growth factor 0.05kg, vitamin B120.4kg, and water 100kg were mixed and stirred for 30 minutes, and sterilized at 121°C and 0.1Mpa for 30 minutes to obtain a fermenter liquid medium; vitamin B12 needs to be sterilized by filtration, and trace elements are iron sulfate, zinc sulfate, One or more of calcium chloride, manganese sulfate, sodium acetate, triammonium citrate, ferric chloride, calcium sulfate, and magnesium chloride.

[0056] Preparation example of Bacillus thuringiensis bacterial liquid The Bacillus thuringiensis in the following raw materials was purchased from the ATC...

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Abstract

The invention relates to the field of biopesticides, and particularly discloses a microorganism-plant two-way insect-resistant fungicide and a preparation method thereof. The insect-resistant fungicide is prepared from, by weight, 5-10 parts of bacillus thuringiensis liquid, 5-10 parts of beauveria bassiana liquid, 10-15 parts of purpureocillium lilacinum liquid and 70-80 parts of plant extract liquid. The preparation method comprises the following steps: weighing the bacillus thuringiensis liquid, the beauveria bassiana liquid, the purpureocillium lilacinus liquid and the plant extract liquid, mixing and stirring to prepare the insect-resistant fungicide. The insect-resistant fungicide has the advantages of being good in compounding effect, stable in mixing, wide in antibacterial spectrum, safe and harmless to human and livestock, and easy to degrade.

Description

technical field [0001] The application relates to the field of biopesticides, more specifically, it relates to a microorganism-plant bidirectional insecticide and a preparation method thereof. Background technique [0002] In modern agricultural production, the ultimate destination of chemical pesticides is to be directly exposed to the ecological environment; from the perspective of the impact of various types of pollutants on terrestrial ecosystems; chemical pesticides are the broadest, with regard to various pollution For the pollution of terrestrial ecosystems by pesticides; chemical pesticides are man-made and the most direct; diffusion and residue are inevitable environmental behaviors of chemical pesticides, and chemical pesticide residues move with the atmosphere and water Migrates over long distances; diffuses from one environmental medium to another; and can affect humans and other animals in areas where pesticides are used long distances through the food chain; du...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/46A01N65/20A01N65/08A01N31/02A01N63/23A01N63/30A01P3/00A01P1/00
CPCA01N65/00A01N65/46A01N65/20A01N65/08A01N31/02A01N63/23A01N63/30
Inventor 屈峰邵晴屈庆涛
Owner 黎昊源生物工程(山东)股份有限公司
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