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Purpureocillium lilacinum and application thereof in chicken feather degradation

A technology of P. lilacinus and chicken feathers, which is applied in the field of biodegradation and can solve the problems of degrading chicken feathers that have not been reported yet.

Inactive Publication Date: 2020-12-15
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fungi of the genus Pururospora are mainly used in the biological control of nematodes, but there is no report on the degradation of chicken feathers

Method used

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  • Purpureocillium lilacinum and application thereof in chicken feather degradation
  • Purpureocillium lilacinum and application thereof in chicken feather degradation
  • Purpureocillium lilacinum and application thereof in chicken feather degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Sample Collection and Isolation Description of the Bacterial Strains

[0019] 1. Experimental materials and methods

[0020] 1.1 Collection of soil samples: collected from the flower bed soil in Kunming City, Yunnan Province, the collection depth was 3-5cm, and the collected soil samples were put into sterile plastic bags and brought back to the laboratory for later use.

[0021] 1.2 Test medium

[0022] Martin's PDA medium: Weigh 200 grams of peeled and washed potatoes, cut into pieces, boil in water for 30 minutes, filter, collect the filtrate, add 20 g of glucose and 20 g of agar powder, stir well, and then autoclave at 121 °C for 30 minutes. Cool to about 40°C, add 1% sterilized Bengal red aqueous solution 3mL, 1% penicillin solution 3mL and 1% streptomycin solution 3mL.

[0023] 1.3 Enrichment and isolation of strains: Mix the soil sample with 10g of chicken feather powder and place it in a petri dish, keep it moist and incubate at a constant temperatur...

Embodiment 2

[0031] Example 2 bacterial strains produce protease initial screening

[0032] 1. Experimental materials and methods

[0033] 1.1 Medium for experiment

[0034] Skimmed milk powder solid medium (g / L): 5g of skimmed milk powder, 20g of agar powder, and 1000mL of water.

[0035] 1.2 Experimental method

[0036] The GZAC18-2JMP strain was spot-inoculated into the center of the solid medium of skim milk powder, and placed in a constant temperature incubator at 25°C. Measure the diameter of the transparent circle and the colony, and calculate the ratio of the two.

[0037] 2. Experimental results

[0038] Such as image 3 As shown, the diameter of the transparent circle produced by the GZAC18-2JMP strain on the degradation of skim milk powder increases with the growth of the colony. By the 7th day, the size of the transparent circle was 41mm, the diameter of the colony was 35mm, and the ratio between the two was 1.17. The results showed that the GZAC18-2JMP strain can degrade...

Embodiment 3

[0039] Example 3 Effect evaluation of P. lilacinus strain on the degradation of chicken feathers

[0040] 1. Experimental materials and methods

[0041]1.1 Treatment of chicken feathers: Chicken feathers were collected in a farmer’s poultry slaughterhouse in Huaxi District, Guiyang City. The collected chicken feathers were washed and soaked in tap water for 12 hours, rinsed with running water, sterilized by autoclaving at 121°C for 30mins, and dried in an oven at 50°C for later use. .

[0042] 1.2 Preparation of fermentation medium

[0043] Chicken feather 10g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.2g, zinc sulfate 0.05g, calcium chloride 0.2g, dilute to 1000mL, pH 7.0, autoclave at 121°C for 30mins, set aside.

[0044] 1.3 Preparation of spore suspension of P. lilacinus strain

[0045] In the ultra-clean workbench, gently scrape off the hyphae of P. lilacensis GZAC18-2JMP strain cultured on PDA medium at 25°C for 7 days, and transfer it to a sterilized tes...

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Abstract

The invention discloses purpureocillium lilacinum and application thereof in chicken feather degradation. The purpureocillium lilacinum is separated from soil in Kunming City, Yunnan Province. The strain is GZAC18-2JMP, and is preserved in the China General Microbiological Culture Collection Center on August 27, 2020, and the preservation number is CGMCC No. 20249. Researches show that the purpureocillium lilacinum is medium-temperature bacteria and can degrade chicken feathers, the optimal culture method for degrading the chicken feathers comprises the following steps of inoculating the GZAC18-2JMP strain into basic fermentation liquor added with the chicken feathers, standing for 48 hours at the temperature of 25 DEG C, and carrying out shaking culture for 96 hours at the speed of 110rpm / min, wherein the chicken feathers in the fermentation liquor are crushed, the soluble protein is 175.7 mu g / mL, and the dry weight of chicken feather filter residues is 0.36 g; and the chicken feather degradation rate is 64%. The strain and the chicken feather degradation culture method thereof can be used for biodegradation of chicken feathers.

Description

technical field [0001] The invention belongs to the field of biodegradation, and in particular relates to a strain of P. lilacinus and its application in degrading chicken feathers. Background technique [0002] Chicken feather is a fibrous and insoluble structural protein rich in keratin, which is the third most abundant polymer in nature after cellulose and chitin. When chicken feathers and other keratin wastes accumulate naturally, they degrade very slowly and easily cause a large amount of accumulation. Nutrient sources, thus causing secondary pollution to the ecological environment and harm to human health. Keratin waste is usually disposed of by landfill, incineration, etc., but incineration will bring safety hazards to the environment and human health, so it is particularly important to use green and healthy methods to degrade keratin. And the degraded chicken feather is a rich source of protein or amino acid, which can be used to produce feed or coating materials a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F15/00C05F11/08A23K20/142A23K10/26A23K10/12C12R1/645
CPCC05F1/005C05F11/08A23K20/142A23K10/26A23K10/12C12R2001/645C12N1/145
Inventor 韩燕峰董醇波牟泉蓉鲁小晓张玲雪陈万浩梁宗琦
Owner GUIZHOU UNIV
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