Plant root-knot nematode biocontrol bacterium as well as preparation and application thereof

A biological control agent and nematode technology, applied in the field of microorganisms, can solve problems such as poor implementation of root-knot nematode disease, low economic value and income, and difficulty in accepting idle land, etc., to achieve strong pathogenicity or kill Elimination effect, not easy to produce drug resistance, fast cultivation effect

Active Publication Date: 2020-01-24
ZHUMADIAN ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, limited by the limited planting area and the high cost investment of greenhouse construction, farmers are affected by the environment and interests, and it is difficult for farmers to accept idle land, or crop rotation with crops with low economic value and low income, so they rely on agriculture to prevent root-knot nematode disease not yet well implemented
[0007] Theoretically, the planting of resistant varieties was once considered to be an effective and widely used control measure to control root-knot nematode diseases. However, the resources of effective resistant varieties in actual agricultural production are limited.

Method used

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  • Plant root-knot nematode biocontrol bacterium as well as preparation and application thereof
  • Plant root-knot nematode biocontrol bacterium as well as preparation and application thereof
  • Plant root-knot nematode biocontrol bacterium as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Porphyridium lavender strain Pt362 Cultivation and domestication

[0036] Original strain of Porphyridium lavender Pl36-1 It was isolated from root-knot nematodes by Wang Mingzu in Hubei Province in 1991 and stored in the Phytopathological Nematode Laboratory of Huazhong Agricultural University. In 1991, he published an article in Acta Phytopathology as "Preliminary Research Report on Root-knot Nematode Egg Parasitic Fungi". Pt362 It is a mutant strain obtained by T-DNA insertion genetic transformation mediated by Agrobacterium tumefaciens. It was deposited in the China Type Culture Collection on November 25, 2016, and its deposit number is CCTCC NO: M2016682 (see Patent Document CN 106967613A).

Embodiment 2

[0037] Example 2: Porphyridium lavender strain Pt362 Determination of egg parasitism and larval lethality of root knot nematodes

[0038] Separate root knot nematode eggs from the roots of diseased tomato plants, disinfect the surface with 1% sodium hypochlorite for 3 minutes and wash 3 times with water. Place the disinfected eggs on top of the hyphae that have been grown on water agar (WA) plates for 2 days. 50 oocysts per dish, sealed and cultured in the dark in a 28°C incubator for 9 days, and observed the parasitic status of the eggs under a microscope. The parasitic observation of egg particles was treated with lactic acid glycerin solution. About 2 minutes after treatment, the edges of the eggs infected by Porphyridium spp. were transparent, and the uninfected ones were still dark. Repeat four times, and only the eggs were used as a blank control.

[0039] Porphyridium lavender strain Pt362 Parasitic root knot nematode eggs such as figure 1 Shown.

[0040] The specific test r...

Embodiment 3

[0050] Example 3: Porphyridium lavender strain Pt362 Spore determination

[0051] Porphyridium lavender Pt362 After culturing on a PDA plate at 28°C for 3 days, use a punch (0.5cm in diameter) to take out the agar block with hyphae on the edge, and transfer it to a petri dish (9cm in diameter) containing quantitative PDA (20mL / dish) ) In the center, inoculate one piece per petri dish to start the strain ( Pl36-1 Strain) is the control, and each strain is repeated 4 times. After incubating at 28°C for 10 days, a spore suspension of P. lavender was prepared, and the spore concentration was determined by counting with a hemocytometer.

[0052] The specific test results are shown in Table 3.

[0053] Table 3 Spore production of Porphyridium lavender

[0054] .

[0055] The determination of spore production of the purple lavender strain showed that: Pt362 Spore production can reach 5.3×10 8 spores / dish; strain Pl36-1 Spore production can reach 3.2×10 6 spores / dish.

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Abstract

The invention discloses a plant root-knot nematode biocontrol bacterium as well as a preparation and an application thereof. The purpureocillium lilacinum CCTCC NO.M2016682 screened through research has strong pathogenicity on plant root-knot nematodes, and can be used as a plant root-knot nematode biocontrol bacterium; a plant root-knot nematode biocontrol agent containing the purpureocillium lilacinum, conidia of the purpureocillium lilacinum, metabolites of the purpureocillium lilacinum or solid fermentation products of the purpureocillium lilacinum can be prepared; the plant root-knot nematode biocontrol agent is applied to soil in a soil mixing form, so that plant root-knot nematode can be effectively prevented and treated; the high-yield and high-quality leucinostatin can be extracted or prepared by using the purpureocillium lilacinum. The biocontrol bacterium and the biological agent are low in production cost, easy to prepare, efficient, low in toxicity, environmentally friendly, free of pollution and not prone to generating drug resistance; the effects of environmental protection, safety, low cost and high-efficiency prevention and treatment of plant root-knot nematodes can be achieved.

Description

Technical field [0001] The invention relates to the technical field of microorganisms, in particular to a plant root-knot nematode biocontrol bacteria, its preparation and its application. Background technique [0002] Root-knot nematodes belong to the genus of Root-knot nematodes in the Class Pleurocytic, Dimensalidae, Heterodermatoideae, and Root-knot nematodes. They are a class of important plant pathogenic nematodes that seriously harm economic crops and are widely distributed throughout the world; It can infect economic food crops, vegetables, ornamental flowers and trees, and even weeds. [0003] In our country, with the rapid development of the agricultural economy, the continuous reform and adjustment of the industrial structure, the continuous and reasonable optimization of the planting system, and the rapid development of the greenhouse and greenhouse cultivation area, coupled with the weak management consciousness of farmers, the incidence of root-knot nematode diseases ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01P5/00C07K7/06C12P21/02C12R1/645
CPCC12N1/14C07K7/06C12P21/02C12R2001/645C12N1/145
Inventor 姜俊李艳杨凡赵红星王勇贺建峰
Owner ZHUMADIAN ACADEMY OF AGRI SCI
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