Paecilomyces lilacinus and application thereof in inhibiting plant growth

A technology of Paecilomyces lilacinus and strains, applied in the field of microorganisms, can solve problems such as the increase of drug-resistant weed populations, excessive pesticide residues in the soil, and river pollution, etc., to prolong the germination time, the effect is obvious, and the effect of inhibiting germination

Active Publication Date: 2020-08-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to farmers' over-reliance and excessive use of chemical herbicides, some social and ecological problems have been neglected by humans for a long time, such as excessive pesticide residues in the soil, the rise of drug-resistant weed popu

Method used

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  • Paecilomyces lilacinus and application thereof in inhibiting plant growth
  • Paecilomyces lilacinus and application thereof in inhibiting plant growth
  • Paecilomyces lilacinus and application thereof in inhibiting plant growth

Examples

Experimental program
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Effect test

Embodiment 1

[0055] The screening, separation and identification of embodiment 1 Paecilomyces lilacinus

[0056] 1. Isolation and purification of bacterial strains

[0057] Wash the stems and roots of purslane (from a certain place in Guangzhou, Guangdong) with tap water, dry them in the air, soak them in 75% (w / w) alcohol for 2 minutes, rinse them with sterile water, and rinse them with 0.1% (w / w) mercuric chloride Soak for 1.5min, rinse with sterile water; wash leaves with tap water, dry, soak in 75% (w / w) alcohol for 2min, rinse with sterile water, soak in 0.1% (w / w) mercuric chloride for 30s, rinse with sterile water rinse. The sterile water-coated plate (LB) cleaned for the last time was used as a blank control to exclude microbial interference on the surface of purslane, and the roots, stems and leaves were cut into small pieces and planted on PDA medium, and cultivated in a 28°C incubator. The grown bacteria were continuously purified by the plate line drawing method until they be...

Embodiment 2

[0067] The influence of different solvent extracts of embodiment 2 Paecilomyces lilacinus culture fluid on plant growth

[0068] (1) Prepare 1 L of PDB culture solution (ie, liquid PDB medium), put it into a 2 L Erlenmeyer flask, seal it, and sterilize it under high temperature and high pressure at 121°C for 30 minutes before use. Under sterile conditions, Paecilomyces lilacinus Scaumcx04 (a strain with a diameter of 0.5 cm) was inoculated in shake flasks filled with PDB culture solution, and then the inoculated shake flasks were placed on a shaker for 7 days, and the shaker The temperature is 28°C and the rotation speed is 180r / min. After culturing for one week, the culture solution was taken out to obtain the corresponding endophytic bacteria solution.

[0069] (2) Filter the obtained bacterial liquid with gauze (to remove large truffles) to obtain the supernatant, and divide it into three equal parts, then add an equal volume of n-butanol, ethyl acetate and petroleum in th...

Embodiment 3

[0077] Embodiment 3, the influence of the ethyl acetate extract of Paecilomyces lilacinus culture fluid on the growth of different plants

[0078] (1) Prepare 1L of PDB culture solution, put it into a 2L Erlenmeyer flask, seal it, and sterilize it under high temperature and high pressure at 121°C for 30 minutes before use. Under sterile conditions, Paecilomyces lilacinus Scaumcx04 (a strain with a diameter of 0.5 cm) was inoculated in shake flasks filled with PDB culture solution, and then the inoculated shake flasks were placed on a shaker for 7 days, and the shaker The temperature is 28°C and the rotation speed is 180r / min. After culturing for one week, the culture solution was taken out to obtain the corresponding endophytic bacteria solution.

[0079](2) Filter the obtained bacterial solution with gauze to obtain a supernatant, and then add an equal volume of ethyl acetate to the supernatant for extraction. After extraction, the obtained organic phase was transferred to ...

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Abstract

The invention discloses paecilomyces lilacinus and application of the paecilomyces lilacinus in inhibiting plant growth. The paecilomyces lilacinus is named as Purpureocillium lilacinum Scaumcx04. Thepreservation number is GDMCC NO: 60794, and the strain is preserved in Guangdong Province Microbial Culture Collection Center on September 27, 2019, wherein the Guangdong Province Microbial Culture Collection Center is located in the 5th floor of the building No. 59 of the courtyard No.100 of Xianlie Middle Road of Guangzhou City. A culture obtained by culturing the paecilomyces lilacinus or spores of the paecilomyces lilacinus and secondary metabolite which is obtained by further organic solvent extraction and chromatographic column purification can inhibit growth of stems on the ground of plants and growth of roots under the ground of the plants, meanwhile, plant seed germination can be inhibited, and the germination time is prolonged, so that the paecilomyces lilacinus can be used as aregulator for plant growth and a plant herbicide, and an effective way is provided for development of plant growth regulators and herbicides.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to a strain of Paecilomyces lilacinus and its application in inhibiting plant growth. Background technique [0002] Farmland weeds are those that grow in the field of crops. Except for the purposefully cultivated plants, other plants in the farmland have survived for a long time by adapting to the ecological conditions of local crops, farming, climate, soil, etc., and compete with cultivated crops in all aspects. , causing damage to crops. According to Li Yanghan's "Chinese Weeds", there are 1387 kinds of weeds in farmland in my country, and there are many kinds of main weeds that cause harm to agricultural production, including 15 kinds of malignant weeds, 31 kinds of dominant weeds, and regional dominant weeds. There are 23 kinds of grasses. From a global perspective, the annual economic loss caused by weed damage in the world is about 40 billion US dollars. In addition to causing ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12P1/02A01N63/30A01P21/00A01P13/00C12R1/79
CPCC12N3/00C12P1/02A01N63/30C12R2001/79C12N1/145
Inventor 颜健黎平白根祥吴丽蓉
Owner SOUTH CHINA AGRI UNIV
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