Purpureocillium lilacinum and application thereof

A technology of P. lilacinus and biological agent, which is applied in the field of bioengineering, can solve the problems of tobacco planting soil and environmental damage, and achieve the effects of improving tobacco agronomic properties, convenient use, and increasing tobacco yield

Active Publication Date: 2017-12-08
广州市博仕奥生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many factors that affect the yield of tobacco planting, including climate, tobacco species, soil, fertilization, pests and diseases, etc. In order to increase tobacco production, a large amount of chemical fertilizers and pesticides are used in the planting process, which accumulate over time and cause damage to the soil and environment of tobacco planting. havoc

Method used

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  • Purpureocillium lilacinum and application thereof
  • Purpureocillium lilacinum and application thereof
  • Purpureocillium lilacinum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Seed medium: sucrose 30g / L, NaNO 3 3g / L, K 2 HPO 4 ·7H 2 O 1g / L, MgSO 4 ·7H 2 O 0.5g / L, KCl 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, the solvent is water.

[0036] Fermentation medium: sucrose 30g / L, soybean powder 4g / L, K 2 HPO 4 ·7H 2 O 3g / L, MgSO 4 ·7H 2 O 0.2g / L, FeSO 4 ·7H 2 O 0.01g / L, solvent is water, pH 7.0.

[0037] Inoculate the activated Purpureocillium lilacinum (Purpureocillium lilacinum) BPT05 with the preservation number GDMCC NO: 60178 in the seed medium, 28°C, 180r / min shaker culture for 24h to obtain the seed liquid, and then press the inoculation amount of 10% (v / v) Inoculated into the fermentation medium, 28 ~ 32 ° C, 200r / min shaker fermentation culture for 96h, the fermentation broth was diluted 20 times, and the 20 times diluted fermentation medium was used as a control. The test seeds were coated seeds of Yunyan 87, refer to GB / T 3543.4-1995 carries out germination test, and the results are shown in Table 1. As can be seen from Table 1, t...

Embodiment 2

[0041] Seed medium: sucrose 30g / L, NaNO 3 3g / L, K 2 HPO 4 ·7H 2 O 1g / L, MgSO 4 ·7H 2 O 0.5g / L, KCl 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, the solvent is water.

[0042] Fermentation medium: sucrose 25g / L, crushed bran 5g / L, NaNO 3 1g / L, K 2 HPO 4 ·7H 2 O 4g / L, MgSO 4 ·7H 2 O 0.2g / L, FeSO 4 ·7H 2 O 0.01g / L, solvent is water, pH 7.5.

[0043] Fermentation: inoculate activated Purpureocillium lilacinum (Purpureocillium lilacinum) BPT05 with the preservation number GDMCC NO: 60178 in the seed medium, 28°C, 200r / min shaker culture for 24h to obtain the seed liquid, and then press 10% (v / v) The inoculum was inoculated into the fermentation medium, the fermentation temperature was 30°C, the aeration was 1.0vvm, the stirring speed was 300r / min, the fermentation was 96h, and the fermentation liquid was harvested.

[0044] Post-processing: add 200g of rice husk powder, 50g of white carbon black, 5g of xanthan gum, and 1g of sodium citrate to each liter of fermentation broth, ...

Embodiment 3

[0046] Seed medium: sucrose 30g / L, NaNO 3 3g / L, K 2 HPO 4 ·7H 2 O 1g / L, MgSO 4 ·7H 2 O 0.5g / L, KCl 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, the solvent is water.

[0047] Fermentation medium: sucrose 30g / L, corn flour 5g / L, K 2 HPO 4 ·7H 2 O 4g / L, MgSO 4 ·7H 2 O 0.3g / L, FeSO 4 ·7H 2 O 0.01g / L, solvent is water, pH 7.2.

[0048] Fermentation: inoculate activated Purpureocillium lilacinum (Purpureocillium lilacinum) BPT05 with the preservation number GDMCC NO: 60178 in the seed medium, 28°C, 200r / min shaker culture for 24h to obtain the seed liquid, and then press 10% (v / v) The inoculum was inoculated into the fermentation medium, the fermentation temperature was 28°C, the aeration was 0.8vvm, the stirring speed was 300r / min, the fermentation was 72h, and the fermentation liquid was harvested.

[0049] Post-processing: add 200 g of crushed bagasse, 5 g of glycerin, and 1 g of sodium citrate to each liter of fermented liquid, stir and mix for 30 minutes, and dry with a dru...

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Abstract

The invention discloses purpureocillium lilacinum and application thereof. The strain is purpureocillium lilacinum BPT05 and was preserved in Guangdong Microbiological Culture Collection Center (GDMCC) of Guangdong Institute of Microbiology, Floor 5, Building 59#, 100#, Xianlie Middle Road, Guangzhou City, and the preservation number is GDMCC NO: 60178. The strain generates substances with the effect of promoting plant growth, in particularly with the effect of remarkably promoting the growth of tobacco. The strain is used for performing liquid fermentation and the fermentation products are subjected to aftertreatment, so that the biological agent for promoting the growth of the tobacco can be prepared; the biological agent for promoting the growth of the tobacco is convenient to storage and transport, convenient to use and stable in activity, and can effectively improve the agronomic trait of the tobacco and increase the yield of the tobacco; and the biological agent for promoting the growth of the tobacco is environment-friendly and pollution-free to soil and environment, and has important significance in agricultural sustainable development.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing a strain of P. lilacensis and a tobacco growth-promoting biological bacterial agent and its application. Background technique [0002] Tobacco is one of the important economic crops in China. In my country, the total tobacco output accounts for about 1 / 3 of the world's tobacco output, ranking first in the world. The tobacco industry has been a large profit and tax household for many years and an important economic source of national and local taxes. There are many factors that affect the yield of tobacco planting, including climate, tobacco species, soil, fertilization, pests and diseases, etc. In order to increase tobacco production, a large amount of chemical fertilizers and pesticides are used in the planting process, which accumulate over time and cause damage to the soil and environment of tobacco planting. severe damage. [0003] B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 廖美德梁锏文杜景德陈泽鹏
Owner 广州市博仕奥生物科技有限公司
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