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Purpureocillium lilacinum with strong virulence on cucumber root-knot nematodes and application thereof

A technology of P. lilacinus and root-knot nematode, which is applied in the field of microorganisms, can solve the problems that the stability of the control effect may not be guaranteed, the complexity of the soil ecological factor environment, and effective control. The effect of fast speed and high spore germination rate

Active Publication Date: 2017-08-18
河南省农业科学院园艺研究所 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the control, due to the complexity of the soil ecological factor environment, the stability of the control effect may not be guaranteed
[0005] At present, the research on the bio-control bacteria of cucumber root-knot nematode is mostly in the laboratory stage, and has not yet achieved effective control in agricultural production. It is urgent to further isolate, screen or induce the domestication of bio-control bacteria that can be effectively applied, and conduct in-depth research on biological potential

Method used

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  • Purpureocillium lilacinum with strong virulence on cucumber root-knot nematodes and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: P. lilacensis bacterial strain Pl36-1-1 the acquisition of

[0017] The original strain of P. lilacinus pl36-1 Mr. Wang Mingzu isolated it from root-knot nematode in Hubei Province in 1991 (refer to the article published in "Acta Phytopathology" in 1991 entitled "A Preliminary Study on Root-knot Nematode Egg-parasitic Fungus"), and it has been preserved in Huazhong Agricultural University. Laboratory of Phytopathological Nematodes. this invention Pl36-1-1 It is a mutant strain obtained by ultraviolet light mutagenesis.

[0018] Mutagenesis method: Carry out ultraviolet mutagenesis according to the method reported by Tanaka et al (1988), roughly as follows: 10mL of P. lilacinus Pl36-1 Conidia suspension (1 x 10 4 conidia / mL) containing Tween 20 (0.02% V / V) was poured into a petri dish with a diameter of 9 cm and exposed to a place about 15 cm away from the ultraviolet lamp (254 nm Phillips TUV 25 W / h), the irradiation time 0.5, 1, 1.5 and 2 minutes re...

Embodiment 2

[0020] Embodiment 2: P. lilacensis bacterial strain Pl36-1-1 Determination of egg parasitism and larval lethality of root-knot nematode

[0021] The root-knot nematode eggs were separated from the root of the diseased plant, and the surface was disinfected with 1% sodium hypochlorite for 3 minutes, washed 3 times with water, and the surface-sterilized eggs were placed on top of the hyphae that had grown on the water agar (WA) plate for 2 days. 50 oocysts were plated, sealed and cultured in a 28°C incubator in the dark for 9 days, and the parasitism of the eggs was observed under a microscope. The parasitic observation of eggs was treated with lactic acid glycerin solution. After about 2 minutes of treatment, the edges of the eggs infected by bacteria were transparent, and the eggs that were not infected were still dark. Repeated four times, and the eggs that were only inoculated were used as the blank control.

[0022] Isolate root-knot nematode larvae from above cucumber roo...

Embodiment 3

[0029] Embodiment 3: P. lilacensis Pl36-1-1 sporulation assay

[0030] P. lilacensis Pl36-1-1 After culturing on the PDA plate at 28°C for 3 days, use a puncher (0.5 cm in diameter) to punch out the agar block with hyphae on the edge, and transfer it to a petri dish (9 cm in diameter) containing quantitative PDA (20 mL / dish). ) center, inoculate one piece in each petri dish to start the strain ( Pl36-1 strain) was used as the control, and each strain was repeated 4 times. Cultivate at 28°C, and after 10 days, prepare a suspension of P. lilacinus spores, count on a hemocytometer, and determine the concentration of spores.

[0031] The sporulation test of the P. lilacinus strain showed that: the strain Pl36-1-1 The amount of spore production can reach 5.9×10 8 spores / dish; strain Pl36-1-1 The amount of spore production can reach 3.2×10 6 spores / dishes. The specific results are shown in Table 3.

[0032] Table 3 Spore production of P. lilacensis

[0033] .

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Abstract

The invention relates to purpureocillium lilacinum with strong virulence on cucumber root-knot nematodes and an application thereof. The strain is Pl36-1-1 and can be developed to a biocontrol agent for biological control of the cucumber root-knot nematodes. Compared with an existing chemical agent for preventing cucumber root-knot nematodes, the purpureocillium lilacinum has the characteristics of being high in efficiency, low in toxicity, few in residues and free of pollution, and being unlikely to generate drug resistance. Besides strong virulence on the cucumber root-knot nematodes, the strain is quickly cultivated, the sporulation quantity is great, the germination rate of spores is high, and the strain is easy to prepare and has good market application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a P. lilacinus ( Purpureocillium lilacinum ) and its applications. Background technique [0002] Cucumber root-knot nematode ( Meloidogyne spp. ) belongs to the class Paracaudalia ( Secernentea ), Pad blade mesh ( Tylenchida ), Heteroderma ( Heteroderoidea ), Cucumber root-knot nematodes ( Meloidogyne ) in Meloidogyne genus Cucumber ( Meloidogyne Goeldi ), is a class of important plant pathogenic nematodes that seriously damage commercial crops and are widely distributed all over the world. Cucumber root-knot nematode has a very wide host range. It can infect not only economical food crops, vegetables and ornamental flowers and trees, but even weeds. Cucumber root-knot nematode disease can reduce the yield of commercial crops by 10-20%, and in severe cases, it can reach more than 75%, and even lead to crop failure. In my country, due to the rapid ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P21/02A01N63/04A01P5/00C12R1/645
CPCA01N63/30C07K7/06C12N1/145C12R2001/645
Inventor 史宣杰杨凡肖炎农马凯赵秀山蔡毓新李艳王彬
Owner 河南省农业科学院园艺研究所
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