A purpureocillium lilacinum-lactobacillus plantarum composite microbe agent and applications thereof

A technology of Lactobacillus plantarum and Violet spore, applied in the field of microorganisms, can solve the problems of high residue, high toxicity, drug resistance and safety, etc., and achieve the effect of broad application prospects.

Inactive Publication Date: 2017-07-04
河南省农业科学院园艺研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the limitations of agricultural control, the lack of resources of resistant varieties and the problems of high toxicity, high residue, drug resistance and safety caused by chemical nematicides, it is urgent to find a better way to control nem...

Method used

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  • A purpureocillium lilacinum-lactobacillus plantarum composite microbe agent and applications thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: A kind of composite bacterial agent (wettable powder) of Purple bacterium lilacinus and Lactobacillus plantarum consists of the following raw materials in parts by weight: 10 parts of Purple purple bacteria, 1 part of Lactobacillus plantarum, 24 parts of diatomaceous earth, 5 parts of gelatin, 12 parts of kaolin, the spore concentration in the said P. lilacinus bacterial agent is not less than 10 9 spores / mL, the spore content in the Lactobacillus plantarum inoculum is not less than 15.0×10 9 cfu / mL.

[0018] The said P. lilacensis is the strain P136-1-1 (preservation number is CCTCC NO: M2016683); the said Lactobacillus plantarum is the ATCC8014 standard strain.

Embodiment 2

[0019] Embodiment 2: A kind of composite bacterial agent (wettable powder) of Purple bacterium lilacinus and Lactobacillus plantarum consists of the following raw materials in parts by weight: 8 parts of Purple purple bacteria, 1 part of Lactobacillus plantarum, 16 parts of diatomaceous earth, 3 parts of gelatin, 10 parts of kaolin, the spore concentration in the said P. lilacinus bacterial agent is not less than 10 9 spores / mL, the spore content in the Lactobacillus plantarum inoculum is not less than 15.0×10 9 cfu / mL.

[0020] The said P. lilacensis is P136-1-1 strain (preservation number is CCTCC NO: M2016683); the said Lactobacillus plantarum is conventional commercial bacterial agent ATCC8014.

Embodiment 3

[0021] Embodiment 3: A kind of composite bacterial agent (wettable powder) of Purple bacterium lilacinus and Lactobacillus plantarum, consisting of the following raw materials in parts by weight: 12 parts of Purple purple bacteria, 1 part of Lactobacillus plantarum, 30 parts of diatomaceous earth, 6 parts of gelatin, 15 parts of kaolin, the spore concentration in the said P. lilacinus bacterial agent is not less than 10 9 spores / mL, the spore content in the Lactobacillus plantarum inoculum is not less than 15.0×10 9 cfu / mL.

[0022] The said P. lilacensis is P136-1-1 strain (preservation number is CCTCC NO: M2016683); the said Lactobacillus plantarum is conventional commercial bacterial agent ATCC8014.

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Abstract

The invention relates to a purpureocillium lilacinum-lactobacillus plantarum composite microbe agent. The composite microbe agent comprises a purpureocillium lilacinum microbe agent and a lactobacillus plantarum microbe agent in a weight ratio of 8-12:1. The spore concentration of the purpureocillium lilacinum microbe agent is not less than 10<9> spore/mL. The spore concentration of the lactobacillus plantarum microbe agent is not less than 15.0*10<9> cfu/mL. Through combination of the purpureocillium lilacinum and the lactobacillus plantarum, soil-borne pathogens can be inhibited, and disease resistance of plants can be improved so as to reduce harm to vegetables, thus promoting vegetable growth and reducing harms caused by root-knot nematode.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a compound microbial agent of P. lilacinus and Lactobacillus plantarum and its application. Background technique [0002] Root-knot nematode disease, also known as "tumor disease", is one of the more serious diseases that occur on vegetables. It mainly harms cucumbers, bitter melons, tomatoes, eggplants, kidney beans, celery, spinach, carrots and other common vegetables. In recent years, with the sustained and rapid development of my country's national economy, the adjustment of the vegetable industry structure, and the rapid expansion of the cultivated area of ​​vegetable protection, the occurrence of root-knot nematode is becoming more and more serious. Generally, the loss of output reaches 30-50%, and even leads to No harvest. In addition, root-knot nematode can also interact with fungi, bacteria, and viruses to form a compound infection, which aggravates th...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N63/00A01N25/14A01P1/00A01P3/00A01P5/00A01P21/00A01C1/06A01G9/10A01G7/06
CPCA01C1/06A01G7/06A01G9/0299A01N25/14A01N63/00A01N63/30
Inventor 杨凡史宣杰马凯蔡毓新赵秀山赵肖斌唐艳领王彬
Owner 河南省农业科学院园艺研究所
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