The invention relates to the technical field of gene sequencing, and provides a parallel gene splicing method based on a De Bruijn graph. The parallel gene splicing method based on the De Bruijn graph comprises the following steps that S1, the distributed De Bruijn graph is built in parallel; S2, error paths are removed; S3, the De Bruijn graph is simplified on the base of a depth graph traversal method; S4, contig is combined, and scaffold is generated; S5, the scaffold is output. The parallel gene splicing method is based on a trunking system, the De Bruijn graph is built in parallel, and the problems that when large genomes are spliced, as the data volume of the large genomes is too large, graphs cannot be built and further processing cannot be executed in traditional single-machine serial gene splicing algorithms are solved. Meanwhile, in the simplifying process, parallel simplification based on depth graph traversal is carried out, the graph simplifying process is simple, the degree of parallelism is high, and the splicing speed is high.