Bio-control composite bacterial agent and preparation process thereof

A compound inoculant and biocontrol technology, applied in the directions of biocides, biocides, plant growth regulators, etc., can solve the problems of accelerated straw decomposition, improper compounding ratio, and poor pertinence, so as to accelerate the growth of crops. , improve soil and reduce pollution

Inactive Publication Date: 2017-06-09
北京金必来生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, most of the decomposing bacteria agents for crop straw returning to the field have problems such as single microbial metabolism type and function, poor pertinence and improper compounding ratio, and cannot achieve fast and efficient straw decomposition effects, so it is difficult to promote and apply them on a large scale
In addition, bacteria, diseases and insect p...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049]A biocontrol composite bacterial agent, comprising components in parts by weight: bacterial species: 8-12 Pseudomonas fluorescens, 18-22 Pseudomonas fluorescens, 18-22 Bacillus cereus, and Bacillus colioids 8-12, Bacillus subtilis 18-22, Bacillus megaterium 8-12, Bacillus thuringiensis 4-6, Trichoderma viride 4-6;

[0050] Medium: beef extract 0.8~1.2, potassium dihydrogen phosphate 2.5~4, urea 1.3~1.8, molasses 10~12, amino acid 1.5~2.5, glucose 2.5~3.5, peptone 0.3~0.6, sodium chloride 0.25~0.4, nitric acid Potassium 0.35-0.6. This embodiment specifically includes the components in parts by weight: strains: P. lilacinus 10, Pseudomonas fluorescens 20, Bacillus cereus 20, Bacillus colloidus 10, Bacillus subtilis 20, Bacillus megaterium Bacillus 10, Bacillus thuringiensis 5, Trichoderma viride 5; medium: beef extract 1, potassium dihydrogen phosphate 3, urea 1.5, molasses 11, amino acid 2, glucose 3, peptone 0.5, sodium chloride 0.3, potassium nitrate 0.5.

[0051] The...

Embodiment 2

[0066] The difference between this embodiment and Embodiment 1 is that the contents of each component are different, and this embodiment includes components in parts by weight:

[0067] Strains: Pseudomonas lilacinus 11, Pseudomonas fluorescens 19, Bacillus cereus 19, Bacillus coliformis 11, Bacillus subtilis 19, Bacillus megaterium 19, Bacillus thuringiensis 5.5, Trichoderma viride 5.5; culture medium : Beef extract 1.1, potassium dihydrogen phosphate 3.5, urea 1.65, molasses 11.5, amino acid 2.2, glucose 3.25, peptone 0.55, sodium chloride 0.35, potassium nitrate 0.55.

[0068] The preparation method of the present embodiment mainly comprises the following steps:

[0069] step one,

[0070] Get the following components by weight to prepare the culture medium:

[0071] Amino acid 2.2, potassium dihydrogen phosphate 2.25, glucose 1.05, peptone 0.55, urea 0.65, beef extract 1.1, adjust the pH to 6.5-7.5 to form a medium;

[0072] Take Pseudomonas fluorescens, Bacillus cereus...

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Abstract

The invention discloses a bio-control composite bacterial agent which comprises, by weight, bacterial strains including 8-12 parts of purpureocillium lilacinum, 18-22 parts of pseudomonas fluorescens, 18-22 parts of bacillus cereus, 8-12 parts of bacillus mucilaginosus, 18-22 parts of bacillus subtilis, 8-12 parts of bacillus megaterium, 4-6 parts of bacillus thuringiensis and 4-6 parts of trichoderma viride and a culture medium comprising 0.8-1.2 parts of beef extract, 2.5-4 parts of potassium dihydrogen phosphate, 1.3-1.8 parts of urea, 10-12 parts of molasses, 1.5-2.5 parts of amino acid, 2.5-3.5 parts of glucose, 0.3-0.6 part of peptone, 0.25-0.4 part of sodium chloride and 0.35-0.6 part of potassium nitrate. The invention further comprises a preparation method of the bacterial agent. The bio-control composite bacterial agent has good effect on solving current problems of soil improvement, environment protection of agriculture and waste straw rubbish and can improve soil and accelerate crop growth and organic matter decomposition.

Description

technical field [0001] The invention relates to the technical field of soil improver, in particular to a biocontrol composite bacterial agent, and also relates to a preparation process of the biocontrol composite bacterial agent. Background technique [0002] As a large agricultural country, crop straw resources are abundant, the quantity is huge, and the variety is diverse. The annual output of crop straw reaches hundreds of millions of tons. Traditional straw is mainly used for gaskets, feeding livestock, and composting manure. In recent years, with the sharp reduction of livestock breeding, a large part of straw has been dumped into the environment in the form of accumulation and incineration, causing serious pollution to the environment. At the same time, it also caused a great waste of resources. [0003] The efficient resource utilization of straw can not only reduce pollution and protect the environment, but also turn waste into treasure and promote sustainable econo...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N63/00A01P21/00A01P5/00A01P7/04A01P1/00C09K17/14C12N1/20C12N1/14C12R1/01C12R1/39C12R1/085C12R1/125C12R1/11C12R1/885C09K101/00
CPCA01N63/00A01N63/30C09K17/14C09K2101/00C12N1/14C12N1/20A01N2300/00
Inventor 付荣军裴德修
Owner 北京金必来生物科技有限公司
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