A strain of P. lilacinus and its application in the control of flea beetle

A technology for flea beetle, lilac violacea, applied in the directions of applications, fungi, microorganism-based methods, etc.

Active Publication Date: 2022-06-14
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fungus is often used to control crop root-knot nematodes, and it is also effective against some insects, but there is no research report on the control of flea beetle

Method used

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  • A strain of P. lilacinus and its application in the control of flea beetle
  • A strain of P. lilacinus and its application in the control of flea beetle
  • A strain of P. lilacinus and its application in the control of flea beetle

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026]Embodiment 1: Isolation and screening of P. lilacensis PlSC59A3 bacterial strain

[0027] Inoculate the slant bacteria of the tested strain on the PDA plate, harvest the conidia after cultivating for 2-3 weeks, suspend the conidia with 0.05% Tween-80 solution, measure the spore concentration by the hemocytometer method, adjust the suspension to 10 8 spores / mL concentration, use the dipping method to soak the flea beetle adults in the conidia suspension for 10s, take out the test insects, dry them, place them in a petri dish with wet filter paper at the bottom, and replace the cabbage leaves every day , and observe the changes of the test insects, pick out the dead adult insects, moisten the culture, etc. when the insects grow moldy, they are determined as dead insects. For every treatment of 10 adults, repeat 3 times. Mortality and adjusted mortality are formally calculated according to:

[0028] Mortality (%) = number of dead insects / number of insects before treatmen...

Embodiment 2

[0034] Embodiment 2: Identification of P. lilacensis PlSC59A3 bacterial strain

[0035] The slants of the P. lilacinium PlSC59A3 strain obtained from the screening in Example 1 were inoculated on a PDA plate, cultured at 27±1° C., and the changes in colony growth were observed. like figure 1 As shown, the initial stage of the colony is white, the surface is fluffy; the colony is cultivated to the 7th day, the colony is round, the center is slightly raised, the middle of the surface is white, the edge is lavender, fluffy, the back is lavender, and the diameter is (42.30± 0.07) mm.

[0036] The obtained P. lilacinium PlSC59A3 bacterial strain of embodiment 1 screening is under an optical microscope, such as figure 2 As shown, the hyphae are septated, branched, transparent, and the width is (2.87±0.11) μm; the conidia are unicellular, oval to round, chain-like, smooth, colorless to pale yellow, 2 μm to 4 μm wide, with bottleneck-shaped irregular branches or whorled branches o...

Embodiment 3

[0038] Embodiment 3: the conidia production of P. lilacensis PlPlSC59A3 bacterial strain

[0039] Plate culture: Take the preserved slant strains, scrape a small amount of conidia and inoculate them on a PDA plate, culture them in an incubator at 27±1°C for 7-14 days, and scrape the conidia on the surface of the colonies for later use.

[0040] Production of pure powder of conidia: suspend the above conidia with 0.05% Tween-80 solution, inoculate them into PD seed culture solution, culture them in shake flasks in an incubator at 27±1°C for 2 days to form seed solution, and inoculate the seed solution On rice culture medium (in plastic bags or solid fermentation tanks), the inoculum amount is about 10% (v / m), fermented at 27±1°C for about 2 weeks, and the conidia powder is collected with a spore separator, packaged and refrigerated, and used for later use .

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Abstract

The invention relates to a P. lilacensis strain and its application in preventing and treating flea beetle, and belongs to the technical field of biological pesticides. The Purpureocillium lilacinum PlSC59A3 strain has been preserved in the Guangdong Microbial Culture Collection Center on October 16, 2020, and the preservation number is GDMCC No: 61232. The PlSC59A3 strain of P. lilacinus provided by the present invention is highly effective in preventing and treating flea beetle. The strain is an entomopathogenic fungus. As a biological pesticide, it has no pollution to the environment, is not easy for pests to develop drug resistance, and is harmless to humans and animals. It is suitable for the production needs of green and healthy agricultural products.

Description

technical field [0001] The invention relates to a P. lilacensis strain and its application in preventing and treating flea beetle, and belongs to the technical field of biological pesticides. Background technique [0002] Phyllotreta striolata belongs to Coleoptera, Chrysophyllidae pests, commonly known as dog lice, vegetable fleas, fleas, soil fleas and yellow fleas, etc., referred to as flea beetle, is an important pest of cruciferous crops in my country. Both larvae and adults of the flea beetle can damage cruciferous crops. The larvae live in the soil and eat the roots and buds of seeds, as well as the roots of plants at the adult stage, causing seedlings to be absent and ridges to break and plants to wilt or even die; adults eat leaves and Tender shoots make the leaves form dense oval perforations, bite off the tender stems of young shoots, and cause the shoots to dry up or even die. Adults have a strong jumping ability to facilitate migration, resulting in an expansio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCA01N63/30
Inventor 胡琼波陈纬翁群芳袁文静
Owner SOUTH CHINA AGRI UNIV
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